[1]李付玻,王奎鹏△,韩立,等.大黄活血化瘀作用的HPLC指纹图谱分析*[J].陕西中医,2018,(11):1646-1648，封3.
　Li Fubo,Wang Kuipeng,Han Li,et al.HPLC fingerprint analysis of promoting blood circulation to remove blood stasis of traditional Chinese medicine rhubarb[J].,2018,(11):1646-1648，封3.
点击复制

大黄活血化瘀作用的HPLC指纹图谱分析*
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2018年11期
页码:
1646-1648，封3
栏目:
方药纵横
出版日期:
2018-12-31

文章信息/Info

Title:
HPLC fingerprint analysis of promoting blood circulation to remove blood stasis of traditional Chinese medicine rhubarb
文章编号:
10.3969/j.issn.1000-7369.2018.11.045
作者:
李付玻1 ; 王奎鹏2△;  韩立3 ; 郭晓娟3 ; 房晓1; 何栋4
1. 河南省南阳市第一人民医院西药科（南阳 473000）， 2.河南中医药大学第一附属医院药学部（郑州450000）， 3.南阳理工学院张仲景国医国药学院（南阳 473000）， 4. 河南省开封市医学科学研究所（开封 475000）
Author(s):
Li Fubo; Wang Kuipeng; Han Li; et al.
Nanyang First People's Hospital, Western Medicine Department (Nanyang 473000)
关键词:
大黄活血祛瘀色谱法;  高压液相@指纹图谱
Keywords:
Rheum officinaleBlood-activating stasis-removingChromatography;  high pressure liquid@ Fingerprint
分类号:
R285
文献标志码:
A
摘要:
目的：研究分析中药大黄活血化瘀作用的高效液相色谱法（HPLC）指纹图谱特征。方法 ：通过大鼠实验分析大黄不同提取部位（系统溶剂法提取）对“血瘀”大鼠血流动力学的影响；通过HPLC分析大黄活血化瘀作用部位的指纹图谱，对照品为大黄素、大黄酚及大黄酸。结果： 与空白对照组比较，模型对照组大鼠全血黏度、红细胞聚集率均显著高（P＜0.05）；与模型对照组比较，大黄正丁醇部位高、低剂量组、大黄50%乙醇部位高、低剂量组大鼠全血粘度、红细胞聚集率均显著低（P＜0.05）；稳定性实验显示主要色谱峰相对保留时间与相对峰面积一致性的相对标准偏差＜2%，精密度实验显示相对标准偏差＜1%，重复性实验显示相对标准偏差＜2%；大黄药材相似度分别为0.978、0.995、0.980、0.956、0.975、0.991、0.970、0.974、0.966与0.993。结论：大黄活血化瘀作用部位包括正丁醇与乙醇，所建立方法较稳定、重复性良好，可用于大黄活血化瘀功效特征指纹图谱监测。
Abstract:
Objective:To study the characteristics of high performance liquid chromatography (HPLC) fingerprint of traditional Chinese medicine rhubarb promoting blood circulation to remove blood stasis.Methods:The effect of different extraction parts of rhubarb (systematic solvent extraction) on the hemodynamics ofrats with blood stasis was analyzed by rat experiment.The fingerprint of rhubarb promoting blood circulation to remove blood stasis site of actionwas analyzed by HPLC. Emodin, chrysophanol and rhein were reference substances.Results:Compared with the blank control group, the whole blood viscosity and erythrocyte aggregation rate were significantly higher in the model control group (P< 0.05).Compared with the model control group, the whole blood viscosity and erythrocyte aggregation rate were significantly lower in rhubarbn-butanol site high and low dose groups and rhubarb 50% ethanol site high and low dose groups (P< 0.05). The stability experiment showed that the relative standard deviation of relative retention time of main chromatographic peak and relative peak area consistency was lower than 2%, while the precision experiment showed the relative standard deviation was lower than 1%, and repeatability experimentshow that the relative standard deviation was lower than 2%.The similarities of rhubarb medicinal materials were 0.978, 0.995, 0.980, 0.956, 0.975, 0.991, 0.970, 0.974, 0.966 and 0.993, respectively.Conclusion:Rhubarb promoting blood circulation to remove blood stasis sites include n-butanol and ethanol. The established method is stable and reproducible, and it can be used for monitoring the effectiveness fingerprint of rhubarbpromoting blood circulation to remove blood stasis.

参考文献/References:

[1] 张向红,程黎晖.大黄的药理作用及临床应用研究进展[J].中国药业,2009,18(21):76-78. [2] 王阳,林鹏程,明升平,等.四种大黄属高山药用植物蒽醌类成分含量的测定[J].湖北农业科学,2017,56(10):1945-1946,1952. [3]巩丹丹,董嘉俊,孙国祥,等.用高效液相色谱组方指纹图谱智能预测中药质量的新模式[J].色谱,2017,35(6):643-649. [4]杨方良,张晶,孙国祥,等.中药组方指纹图谱研究方法和思路[J].色谱,2016,34(7):715-725. [5]杨艳.反相高效液相法测定复方胆通胶囊中大黄酚及大黄素含量[J].实用药物与临床,2013,16(1):45-47. [6]李会芳,王伽伯,金城,等.基于UPLC指纹图谱的市售大黄不同饮片品质研究[J].时珍国医国药,2012,23(9):2314-2316. [7]王宁芳.青海不同产区大黄的HPLC指纹图谱的对比研究[J].安徽农业科学,2014,42(12):3477-3478,3482. [8]蔡惠芬,罗鹏,王珂,等.大黄注射液对犬血管搭桥术后纤溶系统的影响[J].畜牧兽医学报,2010,41(7):897-901. [9]宋雨泽,曾滨阳,任历，等.生物效价法测定大黄炮制品活血化瘀功效[J].中成药,2014,36(9):1921-1924. [10] 宋景春,林青伟,曾庆波，等.大黄素对脓毒症后期小鼠血小板功能的调节作用[J].医学研究生学报,2017,30(7):709-714. [11]关洪月,李林,刘晓,等.中药指纹图谱相似度计算方法探析[J].中国实验方剂学杂志,2011,17(18):292-297. [12] 聂磊,曹进,罗国安,等.中药指纹图谱相似度评价方法的比较[J].中成药,2005,27(3):249-252. [13] 窦志华,乔进,卞理,等.指纹图谱与一测多评法相结合的大黄质量控制方法[J].中国药学杂志, 2015, 50(5):442-448. [14]杜清涛,温金莲,严优芍,等.不同品种不同产地大黄UPLC指纹图谱研究[J].中药材,2013,36(5):725-731. [15] 郭东艳,柳小莉,唐志书,等.大黄不同炮制品化学成分转移规律研究[J].现代中西医结合杂志,2014,23(18):2016-2017.

备注/Memo

备注/Memo:
*河南省科技攻关项目( 122102310008)
更新日期/Last Update: 2018-11-30