[1]杨 旭,翁雅芳,罗模桂,等.白鲜皮联合紫草油治疗大鼠急性湿疹作用机制研究[J].陕西中医,2019,(12):1659-1663.
　YANG Xu,WENG Yafang,LUO Mogui,et al.Study on the mechanism of dictamnus dasycarpus Turcz combined with radix arnebiae oil on atopic eczema rats[J].,2019,(12):1659-1663.
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白鲜皮联合紫草油治疗大鼠急性湿疹作用机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2019年12期
页码:
1659-1663
栏目:
基础研究
出版日期:
2019-12-05

文章信息/Info

Title:
Study on the mechanism of dictamnus dasycarpus Turcz combined with radix arnebiae oil on atopic eczema rats
文章编号:
DOI:10.3969/j.issn.10007369.2019.12.001
作者:
杨 旭1; 翁雅芳2; 罗模桂1; 汪 瑜3; 刘官智1△
1.四川省自贡市第四人民医院（自贡643000）；2.四川省卫生康复职业学院（自贡643000）；3.成都市癌症防治中心（成都市肿瘤质控中心）（成都 610041）
Author(s):
YANG Xu; WENG Yafang; LUO Mogui; et al.
The Fourth People’s Hospital of Zigong City in Sichuan Province（Zigong 643000）
关键词:
急性湿疹; 白鲜皮; 紫草油; 作用机制; 联合用药; 大鼠
Keywords:
Key words Atopic eczema; Dictamnus dasycarpus Turcz; Radix arnebiae oil; Mechanism; Combined use; Rats
分类号:
R758.23
文献标志码:
A
摘要:
摘 要 目的：研究白鲜皮联合紫草油治疗大鼠急性湿疹的作用机制。方法：健康SD大鼠40只随机分为空白组、模型组、白鲜皮组、紫草油组、白鲜皮联合紫草油组，空白组除外，其余组均采用二硝基氯苯（DNCB）建立急性湿疹模型，造模成功后给予相应药物治疗，2次/d，连续12 d，EASI评分，处死大鼠，采样。HE染色观察皮肤组织病理变化；ELISA检测血清IFNγ、IL4、IL31、LTB4、LTC4水平；免疫组化检测皮肤组织CysLTR1、CysLTR2表达；WB检测皮肤组织FLG、PAR2、TRPV1水平。结果：与空白组相比，模型组病理损伤及EASI评分明显增加（P＜005）；与模型组比，联合用药组病理损伤及EASI评分明显减轻；仅联合用药组较模型组明显降低促炎因子IL4、IL31及PAR2/TRPV1通路因子PAR2、TRPV1水平（P＜005）。结论：白鲜皮联合紫草油发挥治疗急性湿疹的作用，其机制可能是通过影响细胞因子及PAR2/TRPV1信号通路，达到对湿疹的治疗作用。 
Abstract:
Abstract Objective：To study the mechanism of dictamnus dasycarpus Turcz combined with radix arnebiae oil on atopic eczema rats. Methods：40 healthy SD rats were randomly divided into blank group，model group，dictamnus dasycarpus Turcz group，radix arnebiae oil group，dictamnus dasycarpus Turcz combined with radix arnebiae oil group，the other groups except the blank group used dinitrochlorobenzene (DNCB) to establish an atopic eczema model. Rats were given appropriate medication after successful modeling，each group was administered twice a day for 12 consecutive days，EASI score and the rats were sacrificed and sampled for examination. The pathological changes of skin tissues were observed by HE staining and EASI score. The levels of serum IFNγ，IL4，IL31，LTB4 and LTC4 were detected by ELISA.The expression of CysLTR1 and CysLTR2 in skin tissue was detected by immunohistochemistry. The levels of FLG，PAR2 and TRPV1 were detected by WB. Results：Compared with the blank group，the pathological injury and EASI scores of the model group were significantly increased (P＜005).Compared with the model group，the pathological injury and EASI scores of the combination group were significantly reduced；only the combination group significantly reduced the levels of proinflammatory factors IL4，IL31 and PAR2/TRPV1 pathway factors PAR2 and TRPV1 (P＜005). Conclusion：Dictamnus dasycarpus Turcz combined with radix arnebiae oil can treat atopic eczema and its mechanism of antieczema may be through affecting serum factor and PAR2/TRPV1 signaling pathway.

参考文献/References:

[1] 郭娟莉,李春鸟.红光照射对慢性湿疹的治疗效果研究[J].陕西医学杂志,2017，46(4):474475.
[2] 武宗琴,彭 勇,王英杰,等.中医内、外治法治疗湿疹的研究进展[J].世界临床药物，2017，17(3):149153.
[3] 孙志高,路军章,周杉京,等.中医药治疗湿疹的研究进展[J].中华中医药杂志，2017，36(8):299301.
[4] 裴小平,杨 斌，陈文静,等.白鲜皮提取液对湿疹模型小鼠血清IL10、IL17和IL22含量的影响[J].皮肤性病诊疗学杂志,2017,24(3):168172.
[5] 章 莉，高宜云,顾科峰,等.复方紫草油对湿疹模型小鼠血清IL2、TNFα水平的影响[J]. 浙江中西医结合杂志,2016,26(9):798800.
[6] 王永慧,王思农,肖静秋,等.藏药二十五味儿茶凝胶对大鼠慢性湿疹模型LTB4、LTC4因子的影响[J].中国皮肤性病学杂志,2018,32(5):568570.
[7] 赵 辨.湿疹面积及严重度指数评分法[J].中华皮肤科杂志,2004,37(1):34.
[8] Lundin S,Wahlgren CF,Bergstra MA，et al. Use of emollients and topical glucocorticoids among adolescents with eczema  datafrom the populationbased birth cohort BAMSE[J]. Br J Dermatol，2018：179.
[9] Wan HL, Chen HZ, Shi XQ. Study on effect of traditional Chinese medicine Jianpi Chushi decoction and ointment on chronic eczema[J].Asian Pacific journal of tropical medicine, 2016, 9(9): 920923.
[10] Zhao JP, Feng XY, Ma XJ. Clinical effects of Wenyang Sanhan Chushi description in rheumatoid arthritis treatment[J]. Pharmacol Clin Chin Materia Medica，2015，1:254255.
[11] 朱颖玲,王 毅,周丽娜,等.中西医结合治疗尿毒症合并皮肤瘙痒症疗效观察[J].陕西中医,2014，35(4):401402.
[12] 孟子琦,吴嘉瑞,杨艳平,等.基于网络药理学的“白鲜皮地肤子”药对治疗湿疹作用机制研究[J].中国医院用药评价与分析,2018,18(6):725728.
[13] Berker M, Frank LJ, Gessner AL, et al. Allergies-AT cells perspective in the era beyond the TH1/TH2 paradigm[J].Clinical Immunology, 2017, 174: 7383.
[14] 李 陈,常 克,杨 静,等.紫草及紫草制剂的抗炎作用研究进展[J].世界中医药,2018，13(6):7478.
[15] 田 波,李春娟.五苓化湿汤治疗儿童顽固型湿疹的疗效及对血清白介素因子的影响[J]. 陕西中医,2017，38(11):15661567.
[16] Baci IS，Ruzicka T. IL31：A new key player in dermatology and beyond.[J]. Journal of Allergy & Clinical Immunology，2018:104109.
[17] 李奇俊,马军党.润燥止痒胶囊联合氯雷他定对慢性湿疹患者应激反应、炎症反应的影响[J].陕西中医,2018,39(10):14471449.
[18] AdamekGuzik T，Guzik TJ，CzerniawskaMysik G，et al. Urinary leukotriene levels are increased during exacerbation of atopic eczema/dermatitis syndrome. Relation to clinical status[J]. Allergy，2015，57(8):732736.
[19] Cascella R，Foti CV，Lepre T，et al. Full sequencing of the FLG gene in Italian patients with atopic eczema：evidence of new mutations,but lack of an association.[J]. Journal of Investigative Dermatology,2011,131(4):982984.
[20] Zhu Y，Underwood J，Macmillan D，et al. Persistent kallikrein 5 activation induces atopic dermatitislike skin architecture independent of PAR2 activity[J]. Journal of Allergy & Clinical Immunology，2017，140(5):207212.

更新日期/Last Update: 2019-12-19