[1]李 玲,呼兴华,吴 永,等.脑灵汤对阿尔兹海默病模型大鼠NFκB和IκB表达的影响*[J].陕西中医,2019,(2):143-147.
　LI Ling,HU Xinghua,WU Yong,et al.Effect of Naoling decoction on expression of NFκB and IκB in Alzheimer’s disease model rats[J].,2019,(2):143-147.
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脑灵汤对阿尔兹海默病模型大鼠NFκB和IκB表达的影响*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2019年2期
页码:
143-147
栏目:
基础研究
出版日期:
2019-02-05

文章信息/Info

Title:
Effect of Naoling decoction on expression of NFκB and IκB in Alzheimer’s disease model rats
文章编号:
DOI: 10.3969/j.issn.10007369.2019.02.002
作者:
李 玲1; 呼兴华2; 吴 永3; 张选国1△
1.陕西省中医医院老年病科 （西安710003）；2. 陕西省中医药研究院文献所 （西安710003）； 3.陕西省中医药大学2016级研究生 （咸阳 712046）
Author(s):
LI Ling; HU Xinghua; WU Yong; et al.
Department of Geriatrics，Shaanxi Provincial Hospital of Traditional Chinese Medicine(Xi’an 710003)
关键词:
脑灵汤; 阿尔兹海默病模型大鼠; 行为学; 形态学; NFκB; IκB
Keywords:
Key words Naoling decoction; Alzheimer’s disease model rats; Behavior; Morphology; NFκB; IκB
分类号:
R741.02
文献标志码:
A
摘要:
摘 要 目的：研究探讨脑灵汤对阿尔兹海默病大鼠模型的保护作用及其对NFκB和IκB表达的影响。方法：采用脑立体定位仪往海马CA3区注射Aβ142建立阿尔兹海默病大鼠模型，通过行为学和形态学实验观察脑灵汤对模型大鼠的保护作用；通过免疫组化染色观察脑灵汤对模型大鼠NFκB和IκB表达的影响。结果：脑灵汤组在电刺激Y迷宫实验成绩考察较模型组优异（P＜0.05）；脑灵汤组大鼠Morris水迷宫实验过程中搜索所需时间较模型组少(P＜0.05)；刚果红染色结果示脑灵汤组海马CA3区未见特征性显示；HE染色结果显示脑灵汤组大鼠海马CA3区细胞形态、层次结果均较模型组好转；免疫组化染色结果显示模型组大鼠海马CA3区NFκB阳性细胞数较正常和假手术组明显增多（P＜0.05），而IκB阳性细胞数结果与之相反，较正常和假手术组减少（P＜0.05），脑灵汤干预治疗后，大鼠海马CA3区内NFκB蛋白表达较模型组变少（P＜0.05），IκB的蛋白表达与之相反，较模型组增加（P＜0.05）。结论：脑灵汤发挥保护阿尔兹海默病模型大鼠的作用，其可能机制是抑制NFκB的表达和提高IκB的表达。
Abstract:
Abstract Objective: To study the protective effect of Naoling decoction on Alzheimer’s disease rat model and its effect on the expression of NFκB and IκB. Methods: A rat model of Alzheimer’s disease was established by injecting Aβ142into hippocampal CA3 area with brain stereotaxic apparatus. The protective effect of Naoling decoction on model rats was observed by behavioral and morphological experiments，and the effect of Naoling decoction on the expression of NFκB and IκB in model rats was observed by immunohistochemical staining. Results: Naoling decoction group was better than model group in Ymaze test results (P＜0.05)；Naoling decoction group spent less time searching Morris water maze test than model group (P＜0.05)；Congo red staining results showed that there was no characteristic expression in CA3 area of hippocampus in Naoling decoction group；HE staining results showed that the morphology and level of cells in Naoling decoction group rats of CA3 area were better than those in model group. Immunohistochemical staining showed that the number of NFκB positive cells in model group of CA3 area of hippocampus were remarkably more than that in normal and shamoperated group (P＜0.05)，while the number of IκB positive cells was significantly lower than that in normal and shamoperated group (P＜0.05). The number of NFκB positive cells in CA3 area of hippocampus in Naoling decoction group was significantly lower than that in model group (P＜0.05)，while the number of IκB positive cells in Naoling decoction were remarkably more than that in model group (P＜0.05). Conclusion: Naoling decoction can protect Alzheimer’s disease rats by inhibiting the expression of NFκB and increasing the expression of IκB.

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备注/Memo

备注/Memo:
*湖南省卫生厅科研课题(2016103)
更新日期/Last Update: 2019-03-13