[1]聂金霞,陈 难,邓克红.当归补血汤对宫颈癌荷瘤小鼠抑瘤效果及对免疫调节作用的研究*[J].陕西中医,2019,(3):279-282.
　NIE Jinxia,CHEN Nan,DENG Kehong..Experimental study on the antitumor effect and immune regulation of Danggui Buxue decoction on cervical cancer bearing mice[J].,2019,(3):279-282.
点击复制

当归补血汤对宫颈癌荷瘤小鼠抑瘤效果及对免疫调节作用的研究*
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2019年3
页码:
279-282
栏目:
基础研究
出版日期:
2019-03-05

文章信息/Info

Title:
Experimental study on the antitumor effect and immune regulation of Danggui Buxue decoction on cervical cancer bearing mice
文章编号:
DOI: 10.3969/j.issn.10007369.2019.03.002
作者:
聂金霞1; 陈 难1; 邓克红2
1.郑州市妇幼保健院产科（郑州 450000）;2.郑州大学第二附属医院妇产科（郑州 450014）
Author(s):
NIE Jinxia; CHEN Nan; DENG Kehong.
Department of Obstetrical，Zhengzhou Maternal and Child Health Hospital（Zhengzhou 450000）
关键词:
当归补血汤; 宫颈癌; 荷瘤小鼠; 抑瘤效果; 免疫调节; 实验研究
Keywords:
Key words Danggui Buxue decoction;  Cervical cancer;  Tumor bearing mice;  Antitumor effect;  Immune regulation; Experimental research
分类号:
R711.74
文献标志码:
A
摘要:
摘 要 目的：探讨当归补血汤对宫颈癌荷瘤小鼠抑瘤效果及免疫调节作用。方法：以宫颈癌荷瘤小鼠为实验模型（小鼠宫颈癌U14细胞接种于Balb/c小鼠双侧大腿内侧皮下），共40只，将其分成两组，每组20只，A组喂药当归补血汤，B组给与等量的蒸馏水灌胃，持续16周，测量治疗前后肿瘤大小，计算肿瘤生长率。在免疫抑制模型基础建立宫颈癌荷瘤小鼠40只，分成两组，每组20只，C组喂药当归补血汤，D组予以等量蒸馏水灌胃，2周后断颈，取胸腺、脾脏称量，计算胸腺指数和脾脏指数。结果：灌胃16周后，A组存活10只，B组存活3只，A组与B组相比，体重较B组明显增加，生存时间明显延长，根据生存状态评分两组综合评分具有统计学差异（P＜0.05）。A组用药4周、8周、12周后肿瘤体积均明显小于B组（P<0.01），A组用药4周、8周后肿瘤生长率明显低于B组（P<0.01），用药12周后肿瘤生长率比较差异无统计学意义（P>0.05）。C组的胸腺指数和脾脏指数显著高于D组，差异比较有统计学意义（P＜0.01）。结论：当归补血汤对宫颈癌荷瘤小鼠抑瘤效果明显，对免疫状态有调节作用。
Abstract:
Abstract Objective: To investigate the antitumor effect and immune regulation effect of Danggui Buxue decoction on cervical cancer bearing mice. Methods: Cervical cancer bearing mice were used as the experimental model (cervix cancer U14 cells inoculated in the medial thigh subcutaneous of Balb/c mice), 40 mices were divided into two groups, 20 mices in each group. Group A was fed with Chinese angelica nourishing blood soup, and group B was given the same amount of distilled water for 16 weeks. The tumor size was measured before and after treatment, and the tumor birth was calculated. At the same time, the tumor bearing mice of cervical cancer were established on the basis of the immunosuppressive model, which were divided into two groups, 20 in each group. Group C was fed with Chinese angelica nourishing blood soup. Group D was given the same amount of distilled water for gastric perfusion. After 2 weeks, the first four of the neck broken, the thymus and spleen were weighed, the thymus index and spleen index were calculated. Results: After 16 weeks of gavage, 10 patients in group A survived, and 3 patients in group B survived. Compared with group B, the weight of group A was significantly higher than that of group B, and the survival time was significantly prolonged. According to the survival status, the scores of the two groups were significantly different. (P< 0.05). The tumor volume of group A was significantly lower than that of group B after 4 weeks, 8 weeks and 12 weeks (P<0.01). The tumor growth rate of group A was significantly lower than that of group B after 4 weeks and 8 weeks (P<0.01). There was no significant difference in tumor growth rate after week (P>0.05). The thymus index and spleen index of group C were significantly higher than those of group D, and the difference was statistically significant (P<0.01). Conclusion: Danggui Buxue decoction has obvious antitumor effect on cervical cancer bearing mice and has a regulatory effect on immune status.

参考文献/References:

[1] 孙晓东,刘 敏,鲍 慧,等.不同年龄段宫颈癌患者的病理特点及预后分析[J] .现代肿瘤医学,2018,26(11):17661769.
[2] 胡春艳,刘 晨,张恩娣,谭宏伟,等.新辅助化疗对Ⅰb2Ⅱb期宫颈癌细胞凋亡以及其预后的影响[J] .陕西医学杂志,2015,44(8):974975.
[3] 任爱玲,周 丽,魏绍斌.中药防治宫颈癌及宫颈HPV感染的研究进展[J] .湖南中医杂志,2018,34(1):175177.
[4] 张奉梅,龚继勇,龚继梅,等.加味当归补血汤对荷瘤小鼠血液流变学及免疫功能的影响[J] .时珍国医国药,2010,21(9) :22472248.
[5] 邝志斌.当归补血汤对病毒性心肌炎小鼠心肌保护作用及对心肌组织血管内皮细胞生长因子(VEGF)表达的影响[J] .黑龙江医学,2017，32(10) :936938.
[6] 王艳杰,杨彦娟,康芯荣,等.当归补血汤对S180荷瘤小鼠免疫器官及IL2影响的研究[J] .中医药学报,2018,33(3):165167.
[7] 张 岳.当归提取物对S180荷瘤模型小鼠肉瘤的抑瘤作用的实验研究[J] .中国民康医学,2016,28 (7):5556.
[8] 刘春萍,李海龙,张 艳,等.当归贝母苦参丸加味方对荷瘤小鼠 H22肝癌肿瘤组织 MMP13和 bFGF 表达的影响[J] ,国际检验医学杂志,2017,38 (2) :153156.
[9] Guo H, Zhang Z, Su Z, et al. Enhanced antitumor activity and reduced toxicity by combination andrographolide and bleomycin in ascitic tumorbearing mice [J] . European Journal of Pharmacology , 2016 , 776 (21) :5263.〖ZK)〗
[10] Jiang S, Qiu L, Li Y, et al. Effects of marsdenia tenacissima polysaccharide on the immune regulation and tumor growth in H22 tumorbearing mice. [J] . Carbohydrate Polymers , 2016 , 137 (2) :5258.
[11] 陈雪霁,王全玉.箬叶多糖对移植性乳腺癌荷瘤小鼠肿瘤的抑制作用及免疫调节机制研究[J] .现代医学,2017,31 (10) :14851488.
[12] Xu Q, Gu J,Lv Y, et al. Angiogenesis for tumor vascular normalization of endostar on hepatoma 22 tumorbearing mice is involved in the immune response [J] . Oncology Letters,2018,15 (3) :3437.
[13] Song L, Liu D, Zhao Y, et al. Sinomenine reduces growth and metastasis of breast cancer cells and improves the survival of tumorbearing mice through suppressing the SHh pathway [J] . Biomedicine & Pharmacotherapy,2018,98(2):687693.
[14] 周丽菁,龙婷婷,周 星,等.刺五加多糖对Lewis荷瘤小鼠抗肿瘤免疫调节作用及机制的研究[J] .中国免疫学杂志,2017,33(6):849853.
[15] 赵 欢,杨东东,郭 强,等.基于“精血髓一体论”观察当归补血汤治疗血管性痴呆临床研究[J] .陕西中医,2016,37(10):13141315，1435.
[16] 宗丽菊.免疫检查点在宫颈癌中的研究进展[J] .现代妇产科进展,2018,27(8):625627,631.
[17] 赵亚丽,王 聪.苦参消癥汤对宫颈癌大鼠模型癌组织中Caspase3、Caspase7、Survivin表达的干预作用[J] .陕西中医,2018,39(5):550552，560.
[18] 窦昊颖,梁芳芳,张盼盼,等.当归补血汤协同肌源性干细胞移植促造血重建的研究:Notch2、Jagged2和Hes5在鼠脾脏中的表达及作用[J] .时珍国医国药,2017,28(12):28712872.
[19] 李 秀,初 杰,李影迪,等.当归/黄芪多糖对腹腔注射环磷酰胺小鼠骨髓造血影响随机平行对照研究[J] .实用中医内科杂志,2017,31(5):5258.
[20] 黄志惠,吴玉霞,费 飞.加味当归补血汤与常规西药治疗肿瘤相关性贫血的临床对比研究[J] .临床和实验医学杂志,2017,16(8):787790.
[21] 石惠燕,张凌燕,田义洲,等.加味当归补血汤对中晚期非小细胞肺癌化疗患者免疫功能及生活质量的影响[J] .新中医,2017,49(3):106108.
[22] 黄小平,李 菲,陈凌波,等.黄芪和当归配伍对环磷酰胺所致骨髓造血功能抑制小鼠造血功能的影响[J] .中草药,2017,48(1):121128.
[23] 宋 磊,姜 波,周忠光.桦褐孔菌醇提物对MFC胃癌荷瘤小鼠抑瘤作用及免疫调节的初步探讨[J] .黑龙江科学,2018,33(1) :6465.
[24] 窦昊颖,张盼盼,梁芳芳,等.Notch2、Notch3、Jagged2和Hes3在当归补血汤协同肌源性干细胞移植鼠骨髓中的表达和作用[J] .时珍国医国药,2016,27(12):30593061.

相似文献/References:

[1]赵欢,杨东东,郭强,等.基于“精-血-髓一体论”观察当归补血汤治疗血管性痴呆临床研究[J].陕西中医,2016,(10):1314.
[2]徐东英,冯相珍.掌叶半夏有效提取物对宫颈癌大鼠癌组织中Survivin、Caspase-3及Caspase-7的影响[J].陕西中医,2019,(7):832.
　XU Dongying,FENG Xiangzhen..Effect of effective extract of Pinellia ternata on Survivin,Caspase -3 and Caspase -7 in carcinoma of cervical cancer rats[J].,2019,(3):832.
[3]孙立哲,李磊强△,耿翠翠,等.圣愈汤联合放射治疗对人宫颈癌荷瘤小鼠脾脏 DCs 细胞功能的影响*[J].陕西中医,2019,(9):1162.
　SUN Lizhe,LI Leiqiang,GENG Cuicui,et al.Effect of combined radiotherapy and Shengyu decoction treatment on function of splenic DCs in cancerbearing mice[J].,2019,(3):1162.
[4]陈十昔,赵 彦,韩宁宁,等.白头翁汤保留灌肠联合rhGMCSF治疗Ⅱ～Ⅲ期宫颈癌盆腔放疗〖JZ〗所致急性放射性直肠炎疗效及对患者炎症反应的影响*[J].陕西中医,2020,(1):46.
　CHEN Shixi,ZHAO Yan,HAN Ningning,et al.Effect of Baitouweng decoction retention enema combined with rhGMCSF on acute radiation 〖JZ〗proctitis caused by pelvic radiotherapy of stage Ⅱ～Ⅲ cervical cancer and its inflammatory response[J].,2020,(3):46.
[5]王小辉,刘紫玲△.贞芪扶正颗粒联合顺铂治疗中晚期宫颈癌术后疗效及对患者T淋巴细胞亚群影响*[J].陕西中医,2020,(6):736.[doi:DOI:10.3969/j.issn.10007369.2020.06.010]
　WANG Xiaohui,LIU Ziling..Application of Zhenqi Fuzheng granules combined with cisplatin regimen in middle and advanced stage cervical cancer after surgery and the influence on T lymphocyte subsets[J].,2020,(3):736.[doi:DOI:10.3969/j.issn.10007369.2020.06.010]
[6]冯方方,吴 琼,鲁昌辉.清宫排毒汤治疗宫颈癌疗效及对患者肿瘤细胞上皮间质转化的影响[J].陕西中医,2021,(2):187.[doi:DOI:10.3969/j.issn.1000-7369.2020.02.013]
　FENG Fangfang,WU Qiong,LU Changhui.Effect of Qinggong Paidu decoction on the treatment of cervical cancer and its impact on the epithelial-mesenchymal transformation of tumor cells[J].,2021,(3):187.[doi:DOI:10.3969/j.issn.1000-7369.2020.02.013]
[7]郝志晔,张 晶,钱 伟,等.参芪排毒汤对宫颈癌组织人乳头瘤病毒E6/E7、P53和PRb基因蛋白表达的影响[J].陕西中医,2021,(2):192.[doi:DOI:10.3969/j.issn.1000-7369.2020.02.014]
　HAO Zhiye,ZHANG Jing,QIAN Wei,et al.Effect and of Shenqi Paidu decoction on the expression of HPV E6/E7,P53 and PRb in cervical cancer tissues[J].,2021,(3):192.[doi:DOI:10.3969/j.issn.1000-7369.2020.02.014]
[8]杨 繁,曾永蕾.苦参碱对宫颈癌细胞中Bcl-2/Bax表达水平及生物学行为的影响[J].陕西中医,2021,(7):840.[doi:DOI:10.3969/j.issn.1000-7369.2020.07.006]
　YANG Fan,ZENG Yonglei.Effect of matrine on Bcl-2/Bax expression level and biological behavior in cervical cancer cells[J].,2021,(3):840.[doi:DOI:10.3969/j.issn.1000-7369.2020.07.006]
[9]吴艳芳,陈 蕾.八珍汤加味联合放疗治疗中晚期宫颈癌疗效及对患者细胞免疫水平和营养状态的影响[J].陕西中医,2022,(8):1052.[doi:DOI:10.3969/j.issn.1000-7369.2022.08.015]

备注/Memo

备注/Memo:
*河南省卫生厅科技攻关项目（201203043）
更新日期/Last Update: 2019-03-13