[1]汤杰印,张 薇,张祥贵,等.商陆皂苷甲对IL-17诱导肾小球系膜细胞增殖的影响*[J].陕西中医,2019,(7):819-822.
　TANG Jieyin,ZHANG Wei,ZHANG Xianggui,et al.Influence of esculentoside A on the proliferation of IL-17 induced glomerular mesangial cells[J].,2019,(7):819-822.
点击复制

商陆皂苷甲对IL-17诱导肾小球系膜细胞增殖的影响*
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2019年7期
页码:
819-822
栏目:
基础研究
出版日期:
2019-07-05

文章信息/Info

Title:
Influence of esculentoside A on the proliferation of IL-17 induced glomerular mesangial cells
文章编号:
DOI:10.3969/j.issn.1000-7369.2019.07.001
作者:
汤杰印; 张 薇; 张祥贵; 徐丽君
遵义医科大学第五附属（珠海）医院肾内科（珠海519100）
Author(s):
TANG Jieyin; ZHANG Wei; ZHANG Xianggui; et al.
Department of Nephrology,Fifth Affiliated Hospital(Zhuhai) of Zunyi Medical University(Zhuhai 519100 )
关键词:
商陆皂苷甲; 肾小球系膜细胞; IL-17; 纤维连接蛋白; 细胞外基质; MTT法
Keywords:
Key words Esculentoside A; Glomerular mesangial cells; Interleukin-17; Fibronectin; Extracellular matrix; MTT assay
分类号:
R692.6
文献标志码:
A
摘要:
摘 要 目的：观察商陆皂苷甲(EsA)对IL-17诱导的肾小球系膜细胞（GMC）增殖的影响，探讨EsA对系膜细胞增殖性肾病的作用机制。方法：将GMC分为对照组(不加药物干预)，IL-17(5 ng/ml)组，IL-17(10 ng/ml)组，IL-17(20 ng/ml)组，IL-17(50 ng/ml)组，IL-17(100 ng/ml)组，同步化后分别培养24、48、72 h，MTT法检测各组对rGMC增殖的影响，确定IL-17诱导rGMC增殖的作用浓度；将rGMC分为对照组（加入IL-17）、干预组（加入IL-17、终浓度为5 mg/L EsA），同步化后分别培养24、48、72 h，MTT法检测各组对GMC增殖的影响，ELISA 法检测各组细胞上清液中纤维连接蛋白（FN）的表达。结果：IL-17各浓度组均促进了GMC增殖（P＜0.05）；EsA抑制了IL-17(100 ng/ml)诱导的GMC增殖（P＜0.05），并下调了FN的表达（P＜0.01）。结论：EsA可抑制IL-17诱导的GMC增殖，并减少FN的分泌。
Abstract:
Abstract Objective:To observe the effect of esculentoside A(EsA) on the proliferation of IL-17 Induced rat glomerular mesangial cells.In order to explore the mechanism of EsA on effects proliferative glomerulonephritis.Methods:The GMC(cell line HBZY-1) divided into control group(only added GMC),IL-17(5 ng/ml) group,IL-17(10 ng/ml) group,IL-17(20 ng/ml) group,IL-17(50 ng/ml) group,IL-17(100 ng/ml) group,with different concentrations of IL-17 stimulation GMC were cultured 24,48,72 h.Detected the proliferation GMC by using MTT assay to determine the IL-17-induced proliferation GMC concentration.The GMC(cell line HBZY-1) divided into control group(added IL-17),the intervention group(IL-17 and adding a final concentration of 5mg/L EsA to intervene),were cultured 24,48,72 h,detected the proliferation GMC by using MTT assay of each group,ELISA method to detect the expression levels of the supernatant fibronectin(FN) of each group.Results:MTT assay the proliferation of IL-17 induced GMC results showed:Compared with the control group,different concentration of IL-17 in each group and were promoted GMC proliferation was statistically significant(P＜0.05).MTT assay the intervention of EsA on GMC proliferation induced by IL-17 results showed:the control group,the intervention group suppressed GMC proliferation(P＜0.05).ELISA assay the level of FN results showed:FN level of the intervention group was higher than the control group,the difference was statistically significance(P＜0.01).Conclusion：EsA can inhibit the proliferation of IL-17 to GMC,and decreased the secretion of FN.

参考文献/References:

[1] Jamba A,Kondo S,Urushihara M,et al.Hydrogen peroxide-inducible Clone-5 regulates mesangial cell proliferation in proliferative glomerulonephritis in mice[J].PloS one,2015,10(4):1415-1417. [2] Ding K,Wang Y,Jiang W,et al.Qian Yang Yu Yin Granule-containing serum inhibits angiotensin II-induced proliferation,reactive oxygen species production,and inflammation in human mesangial cells via an NADpH oxidase 4-dependent pathway[J].BMC Complementary and Alternative Medicine,2015,15(1):81. [3] Kitching AR,Holdsworth SR.The emergence of TH17cells as effectors of renal injury[J].Journal of the American Society of Nephrology,2011,22(2):235-238. [4] Letian Z,Fuyou L.Th17细胞与肾小球疾病的研究进展[J].中南大学学报：医学版， 2013,38(4):432-436. [5] Huenemoerder S,Holzer J,Paust HJ,et al.Characterization of the renal CD4+T-cell response in experimental autoimmune glomerulonephritis[J].Immunology,2012,137:384-385. [6] 薛 茹，王 墨，李 秋.槐耳清膏对IL-17诱导的系膜细胞增殖和分泌纤连蛋白的影响\[C\]//中华医学会第十七次全国儿科学术大会文集.中华医学会儿科专业委员会,郑州，2012:12-13. [7] Hu Z,Qiu L,Xiao Z,et al.Effects of esculentoside A on autoimmune syndrome induced by Campylobacterjejuni in mice and its modulation on T-lymphocyte proliferation and apoptosis[J].International immunopharmacology,2010,10(1):65-71. [8] Liu C,Dong L,Sun Z,et al.Esculentoside A suppresses breast cancer stem cell growth through stemness attenuation and apoptosis induction by blocking IL-6/STAT3 signaling pathway[J].Phytother Res,2018,6:123-124. [9] Zhong W,Jiang L,Wei J,et al.Protective effect of esculentoside A on lipopolysaccharide-induced acute lung injury in mice[J].Journal of Surgical Research,2013,185(1):364-372. [10] 马华林,张欣洲,张祥贵.商陆皂苷甲治疗BXSB狼疮性肾炎小鼠的实验研究[J].广东医学,2011,32(12):1540-1542. [11] Ma HL,Zhang XG,Zhang XZ,et al.The effect of esculentoside A on lupus nephritis-prone BXSB mice[J].Arch Med Sci,2013,9(2):354-360. [12] 张祥贵.商陆皂苷甲对BXSB小鼠肾脏细胞凋亡及Bax和Bcl-2表达的影响[J].广东医学,2013,32(21):2775-2778. [13] 汤杰印,孟令国,张祥贵.商陆皂苷甲对BXSB小鼠肾组织pCNA、Caspase-3、Fas和FasL表达影响[J].实用中医内科杂志,2014,28(5):76-80. [14] 张祥贵,汤杰印.商陆皂苷甲对肾小球系膜细胞增殖的影响[J].陕西中医,2013,34(8):1075-1077. [15] 张祥贵,汤杰印.商陆皂苷甲对IL-1β诱导的肾小球系膜细胞增殖及CDK2、p27的影响[J].重庆医学,2013,42(21):2496-2499. [16] 徐丽君,汤杰印,张祥贵,等.商陆皂苷甲对白细胞介素1β诱导的肾小球系膜细胞增殖及Caspase-3的影响[J].当代医学,2013,19(35):5-7. [17] 汤杰印,董 扬,张祥贵,等.商陆皂苷甲对IL-1β诱导的肾小球系膜细胞ERK通路活化的影响[J].广东医学,2016,37(11):1613-1617. [18] 汤杰印,董 扬,张祥贵,等.商陆皂苷甲对IL-1β诱导的肾小球系膜细ERK1/2-AP1通路活化的影响[J].重庆医学,2017,44(16):2183-2186. [19] 薛 帆,刘百薇,范晶晶.IL-17与自身免疫性疾病关系的研究进展[J].中国当代医药,2010,17(20):16-17. [20] Kwan T,Chadban SJ,Ma J,et al.IL-17 deficiency attenuates allograft Injury and prolongs survival in a murine model of fully MHC-Mismatched renal allograft transplantation[J].American Journal of Transplantation Rearsch,2015. [21] Lu G,Zhang X,Shen L,et al.CCL20 secreted from Ig A1-stimulated human mesangial cells recruits inflammatory Th17 cells in Ig A nephropathy[J].PloS One,2017,12(5):e0178352. [22] Matsumoto K,Kanmatsuse K.Interleukin-17 stimulates the release of pro-inflammatory cytokines by blood monocytes in patients with Ig A nephropathy[J].Scandinavian Journal of Urology and Nephrology,2003,37(2):164-171. [23] 杨青梅.原发性Ig A肾病外周血IL-17及TGF-β1的表达及意义[J].中国临床医学,2013,20(2):154-156. [24] 徐 靓，张慧涛，郑 晶，等.白细胞介素17在狼疮性肾炎小鼠中的表达及抗白细胞介素17抗体的干预作用[J].中国病理生理杂志,2014,30(2):343-346. [25] Xiao ZY,Zheng QY,Jiang YY,et al.Effects of esculentoside A on production of interleukin-1,2,and prostaglandin E2[J].Acta Pharmacologica Sinica,2004,25(6):817-821. [26] 周 倩,姚广涛,金若敏.商陆皂苷甲的肾细胞毒性作用[J].中国药理学与毒理学杂志,2013,28（3）:114. [27] 王 莉.Th17/Treg 细胞失衡在儿童原发性肾病综合征发病中的分子机制研究[D].重庆:重庆医科大学,2010. [28] Yang S,Wang Y,Mei K,et al.Tumor necrosis Factor Receptor 2(TNFR2).Interleukin-17 receptor D(IL-17RD) heteromerization reveals a novel mechanism for NF-KB activation[J].Journal of Biological Chemistry,2015,290(2):861-871. [29] Roussel L,Houle F,Chan C,et al.IL-17 promotes p38 MAPK-dependent endothelial activation enhancing neutrophil recruitment to sites of inflammation[J].The Journal of Immunology,2010,184(8):4531-4537. [30] Meyer DM,Jesson MI,Li X,et al.Anti-inflammatory activity and neutrophil reductions mediated by the JAK1/JAK3 inhibitor,CP-690,550,in rat adjuvant-induced arthritis[J].Journal of Inflammation,2010,7(1):41.

备注/Memo

备注/Memo:
*贵州省科技厅联合基金资助项目（黔科合J字LKZ\[2012\]12）
更新日期/Last Update: 2019-07-12