[1]王漱非,王悦彤,李现成,等.益肾散结复方及其拆方对阿霉素肾病大鼠肾脏组织的影响研究*[J].陕西中医,2020,(1):3-7.
　WANG Shufei,WANG Yuetong,LI Xiancheng,et al.Effects of Yishen Sanjie compound and its detonate prescription 〖JZ〗on kidney tissue of adriamycin nephropathy rats[J].,2020,(1):3-7.
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益肾散结复方及其拆方对阿霉素肾病大鼠肾脏组织的影响研究*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年1期
页码:
3-7
栏目:
基础研究
出版日期:
2020-01-05

文章信息/Info

Title:
Effects of Yishen Sanjie compound and its detonate prescription 〖JZ〗on kidney tissue of adriamycin nephropathy rats
文章编号:
DOI:10.3969/j.issn.10007369.2020.01.001
作者:
王漱非1; 王悦彤2; 李现成1; 张 楠1; 赵亚峰1; 沈 霞2; 毛加荣1; 延 佩1; 王 宇1; 于小勇1; 程小红1; 屈 凯1△
1．陕西省中医医院（西安 710003）；2．陕西中医药大学（咸阳 712046）
Author(s):
WANG Shufei; WANG Yuetong; LI Xiancheng; et al.
Shaanxi Traditional Chinese Medicine Hospital（Xi’an 710003）
关键词:
益肾散结复方; 益肾散结复方拆方; 盐酸贝那普利; 阿霉素肾病; 实验研究; 大鼠
Keywords:
Key words 〖WT5BZ〗〖HT5”SS〗Yishen Sanjie compound; Removing prescription of Yishen Sanjie compound; Benazepril hydrochloride; Doxorubicin nephropathy; Experimental study; Rats
分类号:
R692
文献标志码:
A
摘要:
摘 要 目的：探讨益肾散结复方及其拆方对阿霉素肾病大鼠肾脏组织的影响研究。方法：取健康清洁级SD大鼠70只，随机分为正常对照组、模型对照组、阳性药物对照组、温肾益精化瘀组、滋阴益肾化瘀组、益气化瘀组及全方组。除正常对照组外，其他组建立阿霉素肾病模型。造模成功后，阳性药物对照组给予盐酸贝那普利灌胃；温肾益精化瘀组、滋阴益肾化瘀组、益气化瘀组及全方组给予相应药物连续灌胃，正常对照组与模型对照组，给予等量的0.9%氯化钠溶液灌胃，达疗程后，测定大鼠尿蛋白定量及血清中尿素氮、血肌酐、胆固醇、甘油三酯的浓度，观察电镜下肾脏组织的形态学改变。结果：与模型对照组比较，益肾散结复方全方组及拆方组均能调节脂代谢紊乱、控制蛋白尿、改善肾功能、修复肾脏组织。其中，全方组在控制蛋白尿，改善血清胆固醇、血清甘油三酯、血尿素氮、血肌酐、修复肾脏组织方面显著优于阳性药物对照组。结论：益肾散结复方全方组及拆方组均能有效调节脂代谢紊乱、控制蛋白尿、改善肾功能、修复肾脏组织，为进一步开展细胞实验奠定基础。
Abstract:
Abstract 〖WT5BZ〗〖HT5”SS〗Objective:To investigate the effect of Yishen Sanjie compound and its preparation on renal function in adriamycin nephropathy rats.Methods:70 SD rats of healthy and clean grade were randomly divided into normal control group,model control group,positive drug control group,warming kidney and benefiting essence and removing blood stasis group,nourishing Yin and benefiting kidney and removing blood stasis group,benefiting qi and removing blood stasis group and whole prescription group.Doxorubicin nephropathy model was established in other groups except the normal control group.After successful modeling,benazepril hydrochloride was given to the control group.The kidney essence of blood group,ziyin kidney blood circulation,replenishing qi blood group and the whole party group was given continuous lavage corresponding drugs,normal control group and model control group,gave the same amount of 0.9% sodium chloride solution to fill the stomach,of course,the determination of body mass in rats,24hour urine protein quantitative and serum urea nitrogen,serum creatinine,cholesterol,triglyceride.Results:compared with the model control group,both the whole prescription group and the unrecipe group could regulate the disorder of lipid metabolism,control proteinuria and improve renal function.Among them,the whole prescription group was significantly better than the positive drug control group in controlling albuminuria and improving serum cholesterol,serum triglyceride,blood urea nitrogen and blood creatinine.Conclusion:Yishen Sanjie compound whole prescription and unrecipe can effectively regulate the disorder of lipid metabolism,control proteinuria and improve renal function in rats,laying a foundation for further cell experiments.

参考文献/References:

[1] 祁晓鸣,马俊楠,孟祥龙,等.商陆及其不同炮制品对阿霉素肾病大鼠的作用机制分析[J].中国实验方剂学杂志,2019，25(4)：36.
[2] 屈 凯,王悦彤,王漱非,等.基于文献研究探讨益肾散结法防治肾小球硬化的理论内涵[J].陕西中医,2019,40(7)：919923.
[3] 屈 凯,李现成,张 楠,等.肾复康Ⅱ号胶囊中两种主要药物的有效成分对大鼠肾小球系膜细胞氧化应激反应相关因子的影响[J].中国中西医结合肾病杂志,2016,17(2)：400402.
[4] 屈 凯,延 佩,张 楠,等.肾复康Ⅱ号胶囊治疗慢性肾小球肾炎的疗效观察[J].湖北中医药大学学报,2015,17(5)：3032.
[5] 王漱非,程小红.肾复康Ⅱ号胶囊对阿霉素肾病模型大鼠尿中足细胞计数及骨架蛋白podocin影响的实验研究[J].陕西中医,2014,35(5):609611.
[6] 王漱非.益肾散结法对阿霉素肾病模型大鼠肾组织podocin蛋白及尿中足细胞数量的影响[D].咸阳：陕西中医学院,2014.
[7] 赵亚峰.肾复康Ⅱ号胶囊对肾脏间质纤维化大鼠组织形态和血浆Ang的影响[D].咸阳：陕西中医学院,2010.
[8] 赵亚峰,程小红.程小红辨治慢性肾功能衰竭经验[J].四川中医,2017,35(7):1415.
[9] 赵亚峰,程小红.肾复康Ⅱ号方治疗肾虚血瘀型肾功能衰竭34例[J].陕西中医,2009,30(12):15831584.
[10] 王孙亚,李望辉,韩 忠,等.基于“阴中求阳”理论探讨右归丸及其拆方对肾阳虚证大鼠睾丸组织的影响[J].中华中医药杂志,2018,33(10):43584360.
[11] 史 健,杨洪涛,胡 锐,等.肾复康Ⅱ号抗肾间质纤维化及对人肾上皮小管细胞 转分化的作用[J].陕西中医,2014,35(1):103105.
[12] 蒋宇峰,朱尧焓,陈 潇,等.抗纤灵方对阿霉素肾病大鼠肾纤维化指标的影响[J].吉林中医药,2018,38(12):14301433.
[13] 程小红,邢 斌,刘建红,等.益肾散结法对大鼠肾脏间质纤维化组织TGFβ1和PAI1表达的影响[J].陕西中医,2018,39(2):143145,153.
[14] 王英娟,潘凯丽,强 欢,等.复方肾炎片联合强的松对阿霉素幼鼠肾病模型ET1及TGFβ1表达的影响[J].陕西中医,2014,35(5):627630.
[15] 毛加荣,徐 薇,刘建红,等.益肾散结法对UUO大鼠肾脏间质纤维化的影响[J].陕西中医,2018,39(2):146149.
[16] 王军建,胡 锐,毛云英,等.雷公藤多苷对阿霉素肾病大鼠足细胞病变的影响[J].陕西医学杂志,2011,40(9):11191122，1273.

备注/Memo

备注/Memo:
*国家自然科学基金资助项目（81603454） 陕西省科技攻关计划项目（2017SF295）
更新日期/Last Update: 2020-02-17