[1]蔡之幸,陈 越△.小檗碱联合人参皂苷Rb1对脂肪细胞炎症信号通路作用*[J].陕西中医,2020,(3):297-300.
　CAI Zhixing,CHEN Yue..Effects of berberine and ginsenoside Rb1 on inflammatory signal transduction pathway in adipocytes[J].,2020,(3):297-300.
点击复制

小檗碱联合人参皂苷Rb1对脂肪细胞炎症信号通路作用*
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年3期
页码:
297-300
栏目:
基础研究
出版日期:
2020-03-05

文章信息/Info

Title:
Effects of berberine and ginsenoside Rb1 on inflammatory signal transduction pathway in adipocytes
文章编号:
DOI:10.3969/j.issn.10007369.2020.03.008
作者:
蔡之幸; 陈 越△
上海交通大学医学院附属同仁医院中医科(上海200336)
Author(s):
CAI Zhixing; CHEN Yue.
Department of Traditional Chinese Medicine,Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine(Shanghai 200336)
关键词:
小檗碱;  人参皂苷Rb1;  脂肪细胞;  炎症信号通路;  胰岛素抵抗
Keywords:
Berberine;  Ginsenoside Rb1;  Adipocytes;  Inflammatory signaling path way;  Insulin resistance
分类号:
R282
文献标志码:
A
摘要:
目的:观察小檗碱联合人参皂苷Rb1对脂肪细胞炎症信号通路的影响。方法:将诱导分化成熟的 3T3-L1 脂肪细胞分在肿瘤坏死因子(TNF-α)作用下给予小檗碱、人参皂苷 Rb1、小檗碱和人参皂苷 Rb1合用处理后,观察药物对脂肪细胞炎症信号通路的作用。 结果:基础状态下,与空白对照组相比,Ber组脂肪细胞的TNF-α和IL-6的mRNA表达量降低更明显(P<0.01)。胰岛素抵抗(TNF-α刺激)状态下,与空白对照组比较,Ber组能显著地抑制TNF-α刺激下上调的TNF-α、IL-6的表达(P<0.01),而Rb1仅抑制TNF-α的表达(P<0.01),Ber+Rb1两药合用,与Rb1单用作用类似,仅抑制了TNF-α的表达(P<0.01)。经TNF-α诱导后,加入Ber处理的脂肪细胞,NF-κB和IKK-β 表达水平下调,而加入Rb1处理的脂肪细胞(无论是否+Ber),NF-κB和IKK-β 表达水平上调。 结论:小檗碱和人参皂苷Rb1两者合用对脂肪炎性细胞未能显示出增效作用。
Abstract:
Objective:To observe the effects of ginsenoside Rb1 and its combination with berberine on inflammatory signaling pathways in adipocytes.Methods:The differentiated 3T3 -L1 adipocytes were stimulated with tumor necrosis factor-α(TNF-α),treated with berberine,ginsenosides Rb1,and both berberine and ginsenosides Rb1.The effect of drugs on inflammatory signal pathway of adipocytes was observed.Results:In the basal state,compared with the blank control group,the mRNA expression of TNF - α and IL-6 in the adipocytes of the Ber group decreased more significantly(P<0.01).In the insulin resistance(TNF-α stimulation)state,compared with the blank control group,the Ber group could significantly inhibit the up-regulated expression of TNF-α and IL-6 under the stimulation of TNF-α(P<0.01),while Rb1 only inhibited the expression of TNF-α(P<0.01).The combination of Ber and Rb1,similar to Rb1 alone,only inhibited the expression of TNF-α(P<0.01).After TNF-α induction,the expression levels of NF -κB and IKK-β were down regulated in the adipocytes treated with Ber,while the expression levels of NF -κB and IKK -β were up regulated in the adipocytes treated with Rb1(whether or not + Ber).Conclusions:Berberine combined with ginsenoside Rb1 did not show synergistic effect on adipoinflammatory cells.

参考文献/References:

[1] 黄 伟.葛根芩连汤四种表征成分效阈浓度下配伍的抗肝细胞IR效应及机理[D].北京:北京中医药大学,2018.
[2] 陈 冉,李晓南.棕色脂肪组织分化及调控的研究进展[J].生理科学进展,2016,47(1):38-42.
[3] Choi BH,Ahn IS,Kim YH,et al.Berberin reduces the expression of adipogenic enzymes and inflammatory molecules of 3T3-L1 adipocyte[J].Exp Mol Med,2006,38(6):599-605.
[4] Yi P,Lu FE,Xu LJ,et al.Berberine reverses free-fatty-acid-induced insulin resistance in 3T3-L1 adipocytes through targeting IKKbeta[J].World J Gastroenterol 2008,14:876-883.
[5] Zhang Z,Li X,Lv W,et al.Ginsenoside Re reduce insulin resistant through inhibition of c-jun NH2-terminal kinase and nuclear factor-kappaB[J].Mol Endocrinol,2008,22:186-195.
[6] 刘 莉,蒋昊楠,徐慧荣,等.代谢综合征与肠道菌群的关系及中药防治研究现状[J].中国微生态学杂志,2019,31(5):605-609.
[7] World Health Organization.Definition,diagnosis and classification of diabetes mellitus and its complications:report of a WHO consultation.Part I:diagnosis and classification of diabetes mellitus [M].Geneva:World Health Organization,1999:169-175.
[8] WeiSberg SP,McCann D,Desai M,et al.Obesity is associated with macrophage accumulation in adipose tissue[J].J Clin Invest,2003,112(12):1796-1808.
[9] Xu H,Barnes GT,Yang Q,et al.Chronic inflammation in fat plays a crucial role in development of obesity-related insulin resistance[J].J Clin Invest,2003,112(12):1821-1830.
[10] 李秀钧,郞云红.糖尿病是一种炎症性疾病[J].中华内分泌代谢杂志,2003,19(4):251-253.
[11] Jeong HW,Hsc KC,Lee JW,et al.Berberin suppresses inflammatiory responses through AMPK activation in macrophages [J].Am J Physiol Endocrinol Metab,2009,296(4):955-964.
[12] 赵玉男,谢伟东,刑东明,等.中药有效成分作用靶点研究的策略与实践[J].世界科学技术—中药现代化,2016,18(6):1005-1011.
[13] 周迪夷,牟 新,刘颖慧,等.黄连人参对药对自发性2型糖尿病大鼠脂联素及其受体表达的影响[J].中国中西医结合杂志,2017,37(6):699-703.
[14] 李鹏飞,于 婷,严红实,等.人参协同作用的研究现状[J].食品与机械,2013,29(5):252-255.
[15] 尚文斌,郭 超,赵 娟,等.人参皂苷 Rb1 通过上调脂肪组织葡萄糖转运体促进葡萄糖消耗[J].中国中药杂志,2014,39(22):4448-4452.
[16] Chen WJ,Wang JL,Luo Y,et al.Ginsenoside Rb1 and compound K improve insulin signaling and inhibit ER stress -associated NLRP3 inflammasome activation in adipose tissue[J].J Ginseng Res,2016,40(4):351-358.
[17] 李延晖,张 蓉,杨云霜,等.补肾解毒方影响辐射小鼠TLR4信号通路下游炎性因子表达的机制研究[J].陕西中医,2016,37(9):1256-1258.
[18] 许 可.基于非线性系统分析方法的医学数据挖掘[D].杭州:浙江大学,2008.

备注/Memo

备注/Memo:
*国家自然科学基金资助横向合作课题(TR2017T02)；同仁医院院级课题(TRYJ201707)；同仁医院一般学科建设项目(2017XK32)
更新日期/Last Update: 2020-04-07