[1]卓少华,丘远聪,郑俊德,等.人参皂苷Rg5调控lncRNA-PCAT12抑制胃癌细胞生长实验研究*[J].陕西中医,2020,(4):437-439.
　ZHUO Shaohua,QIU Yuancong,ZHENG Junde,et al.Effect of Ginsenoside Rg5 on lncRNA-PCAT12 on invasion and migration of gastric cancer cells[J].,2020,(4):437-439.
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人参皂苷Rg5调控lncRNA-PCAT12抑制胃癌细胞生长实验研究*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年4期
页码:
437-439
栏目:
基础研究
出版日期:
2020-04-05

文章信息/Info

Title:
Effect of Ginsenoside Rg5 on lncRNA-PCAT12 on invasion and migration of gastric cancer cells
文章编号:
DOI:10.3969/j.issn.10007369.2020.04.009
作者:
卓少华1; 丘远聪1; 郑俊德1; 殷 蕾2; 王惠颖3
1.广东省惠州市第一人民医院药学部(惠州 516003); 2.惠州卫生职业技术学院(惠州 516001); 3.广东省蕉岭县人民医院(蕉岭 514100)
Author(s):
ZHUO Shaohua; QIU Yuancong; ZHENG Junde; et al.
Department of Pharmacy,Huizhou First People's Hospital in Guangdong Province(Huizhou 516003)
关键词:
人参皂苷Rg5;  lncRNA-PCAT12;  胃癌;  细胞增殖;  细胞迁移
Keywords:
Ginsenoside Rg5;  lncRNA-PCAT12;  Gastric cancer;  Cell proliferation;  Cell migration
分类号:
R735.2
文献标志码:
A
摘要:
目的:探讨人参皂苷Rg5通过抑制长链非编码RNA-PCAT12(lncRNA-PCAT12)抑制胃癌(GC)细胞增殖与迁移的价值。方法:对数生长期的人胃癌SNU-1 细胞分为四组,A组,B组与C组加入0.03,0.06,0.09 μmol/L Rg5处理,对照组加入5% DMSO进行处理。MTT法检测细胞增殖、双染法检测细胞凋亡、划痕实验检测细胞迁移、实时定量PCR检测lncRNA-PCAT12表达。结果:处理后24、48、72 h,对照组中lncRNA-PCAT12表达水平和癌细胞增殖指数高于各实验组(P<0.05),C组均低于B组与A组(P<0.05)。实验各组细胞凋亡率均比对照组高(P<0.05),C组比B组与A组高(P<0.05)。转染48 h后,实验各组细胞迁移距离都小于对照组(P<0.05),而C组小于其他两个实验组(P<0.05)。结论:人参皂苷Rg5能通过抑制lncRNA-PCAT12的表达,且高浓度的效果更显著,可通过抑制GC细胞增殖与迁移,促进其凋亡,从而发挥抑癌作用。
Abstract:
Objective:To investigate the inhibitory effect of Ginsenoside Rg5 on the proliferation and migration of gastric cancer(GC)cells by inhibiting long-chain non coding RNA-PCAT12(lncRNA-PCAT12).Methods:SNU-1 cells were divided into 4 groups:group A,group B and group C were treated with 0.03 μ mol / L,0.06 μ mol / L,0.09μ mol / L Rg5 and control group with 5% DMSO.Cell proliferation was detected by MTT assay; Apoptosis was detected by double staining; Cell migration was detected by cell scratch test,and lncRNA-PCAT12 expression was detected by real-time quantitative PCR.Results:The expression level of lncRNA-PCAT12 and cell proliferation index of group B and group A were significantly lower than those of control group(P<0.05),and those of group C were lower than those of group B and group A(P<0.05).The apoptosis rate of group B and group A was significantly higher than that of control group(P<0.05),and that of group C was higher than that of group B and group A(P<0.05).48 hours after transfection,the migration distance of transmembrane cells in experimental group B and experimental group A were significantly less than those in control group(P<0.05),and those in experimental group C were significantly less than those in group B and group A(P<0.05).Conclusion:Ginsenoside Rg5 can inhibit the expression of lncRNA-PCAT12,and the effect of high concentration is more significant.It can inhibit the proliferation and migration of GC cells to promote their apoptosis,and thus play an anti-cancer role.

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备注/Memo

备注/Memo:
*广东省惠州市科技计划项目(2019Y080)
更新日期/Last Update: 2020-04-13