[1]袁 立,余金蓉,李 军,等.陈皮对幼龄大鼠厌食症的改善作用及机制研究*[J].陕西中医,2020,(5):585-588.[doi:DOI:10.3969/j.issn.10007369.2020.05.009]
　YUAN Li,YU Jinrong,LI Jun,et al.Improvement effect and mechanism of Pericarpium cirri reticultae on young anorexia rats[J].,2020,(5):585-588.[doi:DOI:10.3969/j.issn.10007369.2020.05.009]
点击复制

陈皮对幼龄大鼠厌食症的改善作用及机制研究*
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年5期
页码:
585-588
栏目:
基础研究
出版日期:
2020-05-05

文章信息/Info

Title:
Improvement effect and mechanism of Pericarpium cirri reticultae on young anorexia rats
作者:
袁 立1; 余金蓉1; 李 军2; 张欣雨2; 刘建国2
1.湖北省应城市人民医院新生儿科(应城 432400); 2.武汉科技大学附属孝感医院儿科(孝感 432000)
Author(s):
YUAN Li; YU Jinrong; LI Jun; et al.
Department of Neonatal,Yingcheng People's Hospital(Yingcheng 432400)
关键词:
陈皮;  厌食;  胃蛋白酶;  胃酸;  胃动素;  胃泌素;  䥺SymbolbA@-内啡肽;  瘦素
Keywords:
Pericarpium cirri reticultae;  Anorexia;  Pepsin;  Gastric acid;  Motilin;  Gastrin;  β-endorphin;  Leptin
分类号:
R725.7
DOI:
DOI:10.3969/j.issn.10007369.2020.05.009
文献标志码:
A
摘要:
目的:研究陈皮对幼龄厌食大鼠的作用及机制。方法:大鼠随机分为对照组、模型组、三个给药组,每组12只。空白对照组常规饲料喂养,模型组用特制饲料喂养; 在造模8 d后开始给药,对照组和模型组灌胃生理盐水,给药组分别灌胃10、30、50 g/kg的陈皮水煎剂。通过称重法测定大鼠摄食量、体重和胃排空,采用Mett法测定胃蛋白酶活性,中和滴定法测定胃酸含量,试剂盒测定血浆中胃动素、胃泌素和䥺SymbolbA@-内啡肽,蛋白印迹法检测下丘脑中瘦素和神经肽Y蛋白的表达。结果:与模型组相比,给药组大鼠摄食量和体重显著增加,胃蛋白酶活性显著增强,胃酸分泌量明显增多,血浆中胃动素、胃泌素和䥺SymbolbA@-内啡肽含量显著增多,下丘脑中瘦素明显下调、神经肽Y明显上调,胃残留物显著减少(P<0.01); 且效果随着给药量的增加越显著。结论:陈皮呈剂量依赖性改善幼龄大鼠厌食症,主要与增强胃蛋白酶活性,增多胃酸分泌量及血浆中胃动素含量、胃泌素含量、䥺SymbolbA@-内啡肽含量,下调下丘脑中瘦素和上调神经肽Y,促进胃排空有关。
Abstract:
Objective:To investigate the effect and mechanism of Pericarpium cirri reticultae on young anorexia rats.Methods:The rats were randomly divided into a control group,a model group,and three drug-administering groups,12 rats each.The blank control group was fed with normal feed,the model group was fed with special feed,and the administration was started 8 days after modeling.The control group and the model group were given normal saline,and the administration group was intragastrically administered with different doses of dried tangerine peel decoction; the food intake,body weight and gastric emptying of each group were determined by weighing method.Pepsin activity was determined by Mett method,gastric acid content was determined by neutralization titration,plasma motilin,gastrin and 䥺SymbolbA@-endorphin were measured by a kit,and the expression of leptin and neuropeptide Y protein in the hypothalamus was detected by Western blotting.Results:Compared with the model group,the food intake and body weight of the rats in the drug-administered group increased significantly,the pepsin activity increased significantly,the gastric acid secretion increased significantly,the levels of motilin,gastrin and 䥺SymbolbA@-endorphin in plasma increased significantly,leptin was significantly down-regulated and neuropeptide Y was up-regulated in the hypothalamus,and the gastric residue was significantly decreased(P<0.01).The effect was more significant with the increase of the dose.Conclusion:Pericarpium cirri reticultae improves anorexia in young rats in a dose-dependent manner.This is mainly related to enhancing pepsin activity,increasing gastric acid secretion,increasing plasma motilin,gastrin and 䥺SymbolbA@-endorphin content,down-regulating leptin,upregulating neuropeptide Y,and promoting gastric emptying.

参考文献/References:

[1] 蔡 燕,李莲嘉.曲麦枳术汤联合五维赖氨酸治疗小儿厌食症30例[J].陕西中医药大学学报,2019,39(2):101-104.
[2] Lock J.An update on evidence-based psychosocial treatments for eating disorders in children and adolescents [J].J Clin Child Adolesc Psychol,2016,55(10):88-89.
[3] 郝 虎,肖 昕.遗传代谢病诊疗新进展及面临的新挑战[J].分子诊断与治疗杂志,2019,11(1):1-5,38.
[4] 李 军.大黄附子汤治疗危重脓毒症疗效及对患者胃肠功能改善研究[J].陕西中医,2019,40(6):718-721.
[5] 李庆耀,梁生林.陈皮的药用研究进展 [J].中成药,2008,30(2):246-248.
[6] 李 伟,郑天珍,瞿颂义,等.陈皮对小鼠胃排空及肠推进的影响 [J].中药药理与临床,2002,18(2):22-23.
[7] 俞佳妮,尹 业,林海燕,等.肥胖小鼠与非肥胖小鼠皮下前体脂肪细胞分化的差异[J].医学分子生物学杂志,2018,15(4):206-210.
[8] Zhang Y,Li C,Liu Y,et al.Mechanism of extraordinary DNA digestion by pepsin [J].Biochem Biophys Res Commun,2016,472(1):101-107.
[9] 白建伟,李 岩,王 峰,等.绿原酸调控胃癌SGC-7901细胞Bax和Caspase-3蛋白诱导细胞凋亡的作用[J].医学分子生物学杂志,2018,15(3):131-135.
[10] 吴丽萍,王艳丽.小儿增食灵合剂对小儿厌食症动物模型胃蛋白酶活性和胃酸分泌量的影响 [J].中医儿科杂志,2007,3(3):26-28.
[11] 赵 琼,徐世军,霍敏俐.加减益胃汤治疗小儿脾胃阴虚厌食症的实验研究 [J].成都中医药大学学报,2005,28(3):15-17.
[12] 赫 慧,杨 铭,于德伟,等.乐食宝咀嚼片对幼龄厌食症大鼠模型的药理作用[J].中国药师,2017,20(12):2131-2135.
[13] Apu AS,Mondal A,Kitazawa T,et al.Molecular cloning of motilin and mechanism of motilin-induced gastrointestinal motility in japanese quail [J].Gen Comp Endocrinol,2016,233(2):53-62.
[14] 易 燕,李 珍.扶正健脾汤治疗厌食症患儿临床疗效及对NPY、MTL、LP水平影响[J].辽宁中医药大学学报,2018,5(2):215-218.
[15] 吴育美,李继昌,郭文涛.血清胃蛋白酶原、胃泌素17在胃癌筛查中的检测价值[J].陕西医学杂志,2019,48(6):797-799.
[16] 何干强,林 洁,何 媛.董氏开胃贴治疗湿食困脾型厌食症的实验研究 [J].中成药,2017,39(4):822-824.

备注/Memo

备注/Memo:
*湖北省卫生健康委员会科研面上项目(WJ2017M219)
更新日期/Last Update: 2020-05-14