[1]孙豪娴,孙贵香△,邓琳蓉,等.三种不同剂型四君子制剂对脾气虚证大鼠血清SOD活性和MDA含量影响及以方测证研究*[J].陕西中医,2020,(6):724-727.[doi:DOI:10.3969/j.issn.10007369.2020.06.007]
　SUN Haoxian,SUN Guixiang,DENG Linrong,et al.Three forms of Sijun Zi on SOD activity and MDA content in serum of rats with Piqi Xu syndrome and its measurement[J].,2020,(6):724-727.[doi:DOI:10.3969/j.issn.10007369.2020.06.007]
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三种不同剂型四君子制剂对脾气虚证大鼠血清SOD活性和MDA含量影响及以方测证研究*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年6期
页码:
724-727
栏目:
基础研究
出版日期:
2020-06-01

文章信息/Info

Title:
Three forms of Sijun Zi on SOD activity and MDA content in serum of rats with Piqi Xu syndrome and its measurement
作者:
孙豪娴; 孙贵香△; 邓琳蓉; 张 婷; 肖 丹; 厉佳俊
湖南中医药大学(长沙 410208)
Author(s):
SUN Haoxian; SUN Guixiang; DENG Linrong; et al.
Hunan University of Chinese Medicine(Changsha 410208)
关键词:
四君子;  脾气虚证;  超氧化物歧化酶;  丙二醛;  以方测证
Keywords:
Sijun Zi decoction;  Piqi Xu syndrome;  Superoxide dismutase;  Malondialdehyde;  Measurement
分类号:
R289
DOI:
DOI:10.3969/j.issn.10007369.2020.06.007
文献标志码:
A
摘要:
目的:以饮食失节法、耗气破气法、劳倦过度法多因素复合建立脾气虚证大鼠模型,并从大鼠的体重、肛温、血清中SOD活性和MDA含量的变化方面探讨颗粒剂四君子的影响。方法:将50只大鼠随机分为正常组、模型组、四君子汤组、传统四君子膏组、颗粒四君子膏组,每组10只。采用边造模边给药的方法,隔日电子天平称取大鼠重量,数字温度计检测大鼠肛温,28 d后处死大鼠,用ELISA法测定其血清中超氧化物歧化酶(SOD)和丙二醛(MDA)水平。结果:与正常组相比,模型组大鼠的体重、肛温、血清SOD活性均下降,MDA含量增多(P<0.05)。经灌胃四君子制剂药物后,大鼠上述指标均有所恢复,与模型组相比,差异有统计学意义(P<0.05)。汤剂组、传统膏剂组及颗粒剂膏组间比较差异无统计学意义(P>0.05)。结论:四君子汤、传统四君子膏、颗粒四君子膏均有较好的益气健脾的作用,在对大鼠体重、肛温、血清SOD活性和MDA的含量的影响上效应相同。颗粒膏具有制作方便。
Abstract:
Objective:To establish the rat model of Piqi Xu syndrome with dietary loss of control,energy consumption and excessive fatigue,and to explore the effects of Sijun Zi on body weight,anal temperature,SOD activity and MDA content in serum.Methods:50 rats were randomly divided into normal group,model group,Sijun Zi decoction group,traditional Sijun Zi paste group and granule Sijun Zi paste group,with 10 rats in each group.The rats were weighed with an electronic balance on the next day,and the anal temperature was measured with a digital thermometer.After 28 days,the rats were sacrificed,and their serum levels of superoxide dismutase(SOD)and malondialdehyde(MDA)were determined by ELISA.Results:Compared with the normal group,the weight,anal temperature and serum SOD activity of the model group all decreased,while the content of MDA increased(P<0.05).After intragastric administration of Sijun Zi preparation,all the above indexes were recovered in the rats,and the difference was statistically significant compared with the model group(P<0.05).There was no significant difference between decoction group,traditional cream group and granule cream group(P>0.05).Conclusion:Sijun Zi decoction,traditional Sijun Zi ointment and granule Sijun Zi ointment all have good effect of Yiqi Jianpi,and have the same effect on body weight,anal temperature,SOD activity and MDA content of serum in rats.Granular ointment has the characteristics of convenient production.

参考文献/References:

[1] 曾梅艳,陈雪莲,宋厚盼,等.脾气虚证动物模型造模方法与模型评价的研究概述[J].湖南中医药大学学报,2019,39(2):284-289.
[2] 宋 红,郑小伟,王 颖.多因素复合建立脾气虚证大鼠模型及以方测证研究[J].浙江中医杂志,2008,32(3):136-138.
[3] 段杨平,田兆华,李 梅,等.逍遥丸治疗盆腔炎疗效及对患者SOD、MDA、白介素族水平影响的研究[J].陕西中医,2018,39(12):1749-1752.
[4] 牛淑亮,杨 涛,王水泉,等.消肿喷剂对急性骨骼肌损伤小鼠肌组织NO、MDA、SOD表达的影响[J].陕西中医,2017,38(8):1146-1147.
[5] 徐 良.四君子汤对心脏外科术后患者血管活性肠肽与胃肠激素及排便功能影响[J].陕西中医,2018,39(3):316-318.
[6] 李胜酋,李胜愉,张联玲,等.四君子汤加味治疗缺血性脑中风疗效观察[J].陕西中医,2017,38(12):1633-1635.
[7] 马军宏,于向阳,张 楠,等.四君子汤对肠道结构和功能影响研究进展[J].中国中西医结合外科杂志,2015,21(3):328-330.
[8] 尹 玫,窦智勇,张茹玉,等.四君子汤与四君子丸干预脾气虚大鼠宏观表征的比较研究[J].当代医学,2017,23(26):30-32.
[9] 唐华羽,李玉芝,李长德,等.四君子汤总多糖对小鼠肠道菌群及免疫功能的影响[J].陕西中医,2016,37(12):1688-1691.
[10] 谢 伟,宋忠兴,王月茹.白芍配方颗粒的制备工艺研究[J].陕西中医,2017,38(9):1291-1293.
[11] 何佳奇,郑敏霞,王晓鸣,等.浙江省中药配方颗粒使用现状调查与分析[J].浙江中医杂志,2018,53(3):222-223.
[12] 沈光辉,邓雨娇,王静宜,等.基层医院中药饮片与配方颗粒的使用情况对比分析——以重庆某社区医院为例[J].成都中医药大学学报,2018,41(2):68-72.
[13] 闫丽霞.中药制剂技术[M].北京:化学工业出版社,2004:111.
[14] 童宏选.中国膏方源流浅述[J].内蒙古中医药,2012,31(4):135-136.
[15] 王 烁,赵雪琪,杨云祯,等.《临证指南医案》丸剂膏剂与服药法探析[J].天津中医药,2017,34(3):165-167.
[16] 周礼彬,廖嘉宝,王丽丽.中药配方颗粒快速制备中药膏方的方法及质量[J].现代医学与健康研究电子杂志,2019,3(4):22-25.

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备注/Memo

备注/Memo:
*湖南省研究生科研创新项目(2018CX27);华润三九集团横向合作项目(5001/0153)
更新日期/Last Update: 2020-06-09