[1]李 超,宋 伟,彭 冰,等.养阴清热化瘀方对急性放射性肺炎大鼠TLR-4/NF-κB信号通路的影响*[J].陕西中医,2020,(7):864-867.[doi:DOI:10.3969/j.issn.10007369.2020.07.007]
　LI Chao,SONG Wei,PENG Bing,et al.Effect of Yangyin Qingre Huayu recipe on signal pathway of TLR-4/NF-κB in rats with acute radiation pneumonia[J].,2020,(7):864-867.[doi:DOI:10.3969/j.issn.10007369.2020.07.007]
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养阴清热化瘀方对急性放射性肺炎大鼠TLR-4/NF-κB信号通路的影响*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年7期
页码:
864-867
栏目:
基础研究
出版日期:
2020-07-05

文章信息/Info

Title:
Effect of Yangyin Qingre Huayu recipe on signal pathway of TLR-4/NF-κB in rats with acute radiation pneumonia
作者:
李 超1; 宋 伟1; 彭 冰1; 雷 章2; 卢宏达2; 孔庆志2△
1.湖北省荆门市第二人民医院肿瘤内科(荆门448000); 2.华中科技大学同济医学院附属武汉中心医院肿瘤科(武汉430061)
Author(s):
LI Chao; SONG Wei; PENG Bing; et al.
Department of Oncology,Jingmen Second People's Hospital in HuBei Province(Jingmen 448000)
关键词:
急性放射性肺炎;  养阴清热化瘀方;  Toll样受体4;  NF-κB p65;  信号通路;  大鼠
Keywords:
Acute radiation pneumonia;  Yangyin Qingre Huayu recipe;  TLR4;  NF-κB p65;  Rats
分类号:
R563.1
DOI:
DOI:10.3969/j.issn.10007369.2020.07.007
文献标志码:
A
摘要:
目的:探讨养阴清热化瘀方对大鼠急性放射性肺炎的防护作用及可能机制。方法:采用随机数字表法将48只雌性Wistar大鼠随机分为空白对照组(NC)、模型组(MD)、养阴清热化瘀方提前干预组(YHF-EI)及养阴清热化瘀方组(YHF-ST),分别于照射后4周时采集血清及取出右肺。观察大鼠的一般情况,应用HE染色观察肺组织病理改变,ELISA法检测血清中含量, RT-PCR和Western-blotting法分别检测肺组织内TLR4、NF-κB p65 mRNA和蛋白的水平。结果:养阴清热化瘀方能显著减轻大鼠放射性肺炎的炎症表现; 与空白对照组比较,血清中TNF-α、IL-6、TGF-β的含量升高,TLR4和NF-κB p65 mRNA转录活性增强,肺组织中TLR4和NF-κB p65的蛋白表达水平升高; 与模型组比较,两组养阴清热化瘀方组血清中TNF-α、IL-6、TGF-β的含量降低,TLR4和NF-κB p65 mRNA转录活性下降,肺组织中TLR4和NF-κB p65的蛋白表达水平降低。结论:养阴清热化瘀方可能通过抑制“TLR4/NF-κB”信号通路,从而抑制TNF-α、IL-6、TGF-β的产生,起到抵抗放射性肺炎的发生,从而发挥放射防护作用。
Abstract:
Objective:To investigate the protective effect and possible mechanism of Yangyin Qingre Huayu recipe on acute radioactive pneumonia in rats.Methods:48 female Wistar rats were randomly divided into blank control group(NC),model group(MD),Yangyin Qingre Huayu recipe group(YHF-EI)and Yangyin Qingre Huayu recipe group(YHF-ST).Serum and right lung were collected at 4 weeks after irradiation.The general conditions of the rats were observed.Pathological changes of lung tissues were observed by HE staining,serum was detected by ELISA,and the levels of TLR4 and NF-κB p65mRNA and protein were detected by rt-pcr and western-blotting respectively.Results:Yangyin Qingre Huayu recipe can significantly reduce the inflammation of radioactive pneumonia in rats.Compared with the control group,the serum levels of tnf-alpha,IL-6,and TGF-β were increased,and the transcriptional activity of TLR4 and kappa B p65mRNA was enhanced,and the protein expression levels of tlr4tnf-alpha,IL-6,TGF- β,and kappa B were increased.Compared with the model group,the levels of TNF-α,IL-6,and TGF- β in serum were decreased,and the transcriptional activity of TLR4 and NF-κB p65 mrna was decreased,and the protein expression levels of TLR4 and NF-κB p65 in lung tissues were decreased.Conclusion:Yangyin Qingre Huayu recipe may inhibit the production of TNF-a,IL-6 and TGF-β by inhibiting the “TLR4/NF-κB” signaling pathway,and thus play a role in preventing the occurrence of radioactive pneumonia,and thus play a role in radiation protection.

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备注/Memo

备注/Memo:
*湖北省自然科学基金资助项目(2017CFB408)；湖北省荆门市重点科技攻关项目(YFZ2017044)
更新日期/Last Update: 2020-07-09