[1]樊一波,文颖娟△,李 蒙,等.以脾胃为中心的葛根相关配伍对糖尿病心肌病大鼠受损心肌线粒体的改善作用*[J].陕西中医,2020,(10):1351-1355.[doi:DOI:10.3969/j.issn.1000-7369.2020.10.002]
　FAN Yibo,WEN Yingjuan,LI Meng,et al.Improving effect of Pueraria-related compatibility centered on spleen and stomach on injured myocardial mitochondria in DCM rats[J].,2020,(10):1351-1355.[doi:DOI:10.3969/j.issn.1000-7369.2020.10.002]
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以脾胃为中心的葛根相关配伍对糖尿病心肌病大鼠受损心肌线粒体的改善作用*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年10期
页码:
1351-1355
栏目:
基础研究
出版日期:
2020-10-05

文章信息/Info

Title:
Improving effect of Pueraria-related compatibility centered on spleen and stomach on injured myocardial mitochondria in DCM rats
作者:
樊一波1; 2; 文颖娟1△; 李 蒙1; 李 静1; 朱瑞超1
1.陕西中医药大学(咸阳712046); 2.宝鸡职业技术学院(宝鸡 721013)
Author(s):
FAN Yibo; WEN Yingjuan; LI Meng; et al.
Shaanxi University of Chinese Medicine(Xianyang 712046)
关键词:
葛根;  配伍;  糖尿病心肌病;  线粒体;  改善作用;  大鼠
Keywords:
Pueraria lobata;  Compatibility;  Diabetic cardio myopathy;  Mitochondrial quality;  Improvement;  Rats
分类号:
R587.2
DOI:
DOI:10.3969/j.issn.1000-7369.2020.10.002
文献标志码:
A
摘要:
目的:观察葛根及其配伍对糖尿病心肌病(DCM)大鼠心肌线粒体质量变化的影响,探寻治疗糖尿病心肌病的最优配伍及机制。方法:采用链脲佐菌素腹腔注射进行模型大鼠制备,并将60只成模大鼠随机分为模型组6.5 g/(kg·d)、达美康组16.7 mg/(kg·d)、葛根组1.04 g/(kg·d)、葛芪组2.6 g/(kg·d)、葛参组2.6 g/(kg·d)、葛蒌组4.48g/(kg·d)及葛芪参蒌组7.6 g/(kg·d)各8只,同时选取空白大鼠8只,为对照组6.5 g/(kg·d)。各组依据上述剂量,以相应药物持续灌胃9周后,对比各组心肌线粒体超微结构变化、心肌线粒体膜电位变化及心肌ATP变化情况。结果:电镜显示各药物干预组心肌线粒体超微结构较模型组均有不同程度的改善,其中葛芪参蒌组改善效果最为明显,与空白组无明显差异; 流式细胞仪检测显示:各干预组波峰均在模型组、空白组之间; 其中葛芪参蒌组、葛参组、葛根组波峰位于达美康组右侧; 葛芪组、葛蒌组则位于达美康组左侧; 以葛芪参蒌组波峰与空白组最为接近。心肌ATP浓度检测显示:各中药干预组心肌ATP浓度均较模型组有所提升(P<0.05),其中葛芪参蒌组ATP值较其余各干预组提升最为明显(P<0.05),但仍低于空白组(P>0.05)。结论:葛根相关配伍均能够对心肌线粒体结构、心肌线粒体膜电位进行正向调控,进而使因糖尿病心肌病导致的心肌线粒体受损得以恢复,其中以益气、化瘀、祛痰三法合用的组方原则进行配伍的葛芪参蒌方改善效果最优。可见其治疗机制可能是通过益气、化瘀、祛痰的协同作用,正向调控心肌线粒体质量而达到治疗目的。
Abstract:
Objective:To observe the effects of Pueraria and its compatibility on the changes of myocardial mitochondria in rats with diabetic cardiomyopathy,and to explore the optimal compatibility and mechanism for the treatment of diabetic cardiomyopathy.Methods:Model rats were prepared by intraperitoneal injection of STZ,and 60 model rats were randomly divided into a model group 6.5 g/(kg·d),a Damicon group 16.7 mg/(kg·d),and a Pueraria group 1.04 g/(kg·d),Geqi group 2.6 g/(kg·d),Geshen group 2.6 g/(kg·d),Gelou group 4.48 g/(kg·d),and Geqishenlou group 7.6 g/(kg·d)8 rats each,and 8 blank rats were selected as control group 6.5 g/(kg·d).According to the above doses,each group was continuously administrated with the corresponding drugs for 9 weeks,and the changes of myocardial mitochondrial ultrastructure,myocardial mitochondrial membrane potential and myocardial ATP were compared in each group.Results:Electron microscopy showed that the myocardial mitochondrial ultrastructure of each drug intervention group was improved to a different extent than the model group,and the improvement effect of Geqi shenlou group was the most obvious,and there was no significant difference from the blank group.Flow cytometry showed that the peaks of each intervention group were between the model group and the blank group.The peaks of the Geqi shenlou group,the Geshen group,and the Gegen group are located on the right side of the Damekang group.The Geqi group and Gelou group were located on the left side of Damicon group; The peaks of Geqi shenlou group were closest to the blank group.Measurement of myocardial ATP concentration showed that the myocardial ATP concentration of each Chinese medicine intervention group was increased compared with the model group(P<0.05),and the ATP value of Geqi Shenlou group was most significantly improved than the other intervention groups(P<0.05),but still lower than the blank group(P> 0.05).Conclusion:Pueraria related compatibility can positively regulate myocardial mitochondrial structure and myocardial mitochondrial membrane potential,thereby recovering myocardial mitochondrial damage caused by diabetic cardiomyopathy.The improvement effect of Geqi Shenlou recipe on principle is the best.The therapeutic mechanism may be achieved through the synergistic effects of benefiting qi,removing blood stasis and expectorant,and positively regulating the quality of myocardial mitochondria.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目(81202642)；陕西省科学技术厅资助项目(2017SF-323)；第四批全国中医(临床、基础)优秀人才研修项目(国中医药人教发[2017]24 号)
更新日期/Last Update: 2020-10-10