[1]赵 茜,吴佳丽,余文丽,等.清热润燥方对哮喘小鼠气道反应及炎性反应影响的实验研究*[J].陕西中医,2020,(12):1695-1698,1707.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.004]
　ZHAO Qian,WU Jiali,YU Wenli,et al.Experimental study on the effect of Qingre Runzao recipe on airway reaction and inflammatory reaction in asthmatic mice[J].,2020,(12):1695-1698,1707.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.004]
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清热润燥方对哮喘小鼠气道反应及炎性反应影响的实验研究*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年12期
页码:
1695-1698,1707
栏目:
基础研究
出版日期:
2020-12-05

文章信息/Info

Title:
Experimental study on the effect of Qingre Runzao recipe on airway reaction and inflammatory reaction in asthmatic mice
作者:
赵 茜1; 吴佳丽1; 余文丽1; 魏丹霞2△
1.云南中医药大学第三附属医院(昆明 650011); 2.昆明市中医医院(昆明 650011)
Author(s):
ZHAO Qian; WU Jiali; YU Wenli; et al.
The Third Affiliated Hospital of Yunnan University of Traditional Chinese Medicine(Kunming 650011)
关键词:
清热润燥口服液;  哮喘;  黏蛋白5AC;  水通道蛋白5
Keywords:
Qingre Runzao oral liquid;  Asthma;  Mucin 5AC;  Aquaporin 5
分类号:
R256.12
DOI:
DOI:10.3969/j.issn.1000-7369.2020.12.004
文献标志码:
A
摘要:
目的:观察清热润燥口服液对哮喘小鼠模型的气道反应、肺组织病理学、气道炎症中IL-13诱导表达的黏蛋白5AC(MUC5AC)和水液代谢的水通道蛋白(AQP5)等因子的影响,探讨“润养与通利”辨治哮喘的理论。方法:采用卵蛋白制造小鼠哮喘模型。将72只小鼠随机分为正常对照组、模型对照组、阳性药物对照组,清热润燥口服液高、中、低剂量组,各组药物作用后观察相关指标。结果:与模型组比较,清热润燥低剂量组吸气峰流速减少,气道炎症稍好转,BALF中IL-13、MUC5AC均减少; 清热润燥中、高剂量组及阳性药物组吸气峰流速增加、气道炎症好转,IL-13、MUC5AC均减少,AQP5增加。结论:清热润燥方在改善哮喘小鼠气道炎性反应、减少MUC5AC及增加AQP5的表达方面具有积极作用。
Abstract:
Objective:To observe the airway response of Qingre Runzao oral liquid to asthma mouse models,lung histopathology,expressed by IL-13 in airway inflammation,mucin MUC5AC induced,the impact of the factors such as aquaporin AQP5 influencing respiratory metabolism,and to explore the theory of “Runyangyu Tongli” for treatment differentiation of asthma.Methods:Egg protein was used for making mouse asthma model.72 mice were randomly divided into normal control group,model control group,positive drug control group,and Qingre Runzao oral liquid high,medium and low dose groups.The relevant indicators of each group were observed after drug action.Results:Compared with the model group,the inspiratory peak flow rate was decreased,airway inflammation was slightly improved,and IL-13 and MUC5AC in BALF were decreased in the low dose group.The inspiratory peak flow rate was increased,airway inflammation was improved,IL-13 and MUC5AC were decreased and AQP5 was increased in the medium and high dose groups and the positive drug group.Conclusion:Qingre Runzao recipe has positive effect on improving the airway inflammatory response of asthmatic mice,reducing MUC5AC and increasing the expression of AQP5.

参考文献/References:

[1] 唐徐韵,陈盼碧.穴位埋线治疗支气管哮喘的实验研究探讨[J].陕西中医,2019,40(12):1789-1791.
[2] Kewu H,Ting Y,Jianying X,et al.Prevalence,risk factors,and management of asthma in China:a national cross-sectional study[J].The Lancet,2019,394(8):407-418.
[3] 魏丹霞,谭艳云,赵 扬,等.名老中医陆家龙“润养”与“通利”治疗慢性咽炎经验[J].环球中医药,2018,11(1):91-92.
[4] 陈 斌,顾力华,魏丹霞,等.陆家龙运用《黄帝内经》思想指导临床辨证经验[J].环球中医药,2019,12(11):1725-1728.
[5] 汤家铭,陈民利.医学实验动物学[M].北京:中国中医药出版社,2008:34.
[6] 焦文妤,张福康.多索茶碱和氨茶碱静脉滴注与糖皮质激素类药物治疗支气管哮喘急性发作临床效果及安全性对比研究[J].陕西医学杂志,2018,47(9):1185-1188.
[7] 陈 昕,张 莹.血液透析联合血液灌流对尿毒症患者毒素清除率、炎性因子、钙磷代谢及免疫功能的影响[J].陕西医学杂志,2019,48(6):770-773.
[8] 付迎新,张王梅,李学勤.肺炎支原体肺炎患儿免疫功能及炎性因子的变化研究[J].北京医学,2018,40(7):657-660.
[9] 吴 铭,冯宗太,马淑蓉,等.脓毒症患儿血浆炎性因子的表达水平[J].江苏医药,2019,45(4):336-338.
[10] 汤呈怀,佘崇阳,万立华.大鼠溺死内参基因筛选和水通道蛋白5的表达[J].重庆医科大学学报,2018,43(1):103-106.
[11] 慢性气道炎症性疾病气道黏液高分管理专家组.慢性气道炎症性疾病气道黏液高分泌管理中国专家共识[J].中华结核和呼吸杂志,2015,38(10):723-729.
[12] 李永明,薛 鸾.AQP5在干燥综合征发病机制中的作用[J].中华中医药学刊,2019,37(10):2369-2372.
[13] 杜丽君,王荣丽.黏蛋白MUC5AC与肺部疾病的研究进展[J].国际呼吸杂志,2014,34(4):315-318.
[14] 黄翠平,周京国.水通道蛋白-5及其信号通路在原发性干燥综合征发病中的作用[J].中华临床医师杂志:电子版,2013,12(9):4036-4038.
[15] 罗建江,马红霞.竹沥对慢阻肺大鼠肺组织中AQP1、AQP4、AQP5、TNF-α和IL-6的影响[J].陕西中医,2017,38(11):1617-1619.
[16] 李 波,陈 东,王桂芳,等.水通道蛋白1、3、4、5在内毒素性急性肺损伤小鼠肺组织中的表达[J].第二军医大学学报,2008,29(2):131-135.
[17] 李永明,薛 鸾.AQP5在干燥综合征发病机制中的作用[J].中华中医药学刊,2019,37(10):2369-2372.
[18] 刘荣奎,王玉娟.肺科痰证辨析[J].山东中医杂志,2008,27(2):135-136.
[19] 姜莉芸,魏丹霞,施兴黔,等.陆家龙主任医师学术思想与临证经验撷要[J].云南中医中药杂志,2010,31(10):5-8.

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备注/Memo

备注/Memo:
*云南省教育厅科学研究基金资助项目(2019Y0330)；云南省科技厅中医联合面上项目[2019FF002(-033)]
更新日期/Last Update: 2020-12-11