[1]安 阳,马武开,黄 颖,等.侗医药松杖方对类风湿关节炎患者滑膜细胞STAT3炎症信号表达的影响[J].陕西中医,2021,(1):8-11.[doi:DOI:10.3969/j.issn.1000-7369.2020.01.002]
　AN Yang,MA Wukai,HUANG Ying,et al.Effect of Dong medicine Usnea & Polygonum cuspidatum formula on STAT3 inflammation signal expression in synovial cells of patients with rheumatoid arthritis[J].,2021,(1):8-11.[doi:DOI:10.3969/j.issn.1000-7369.2020.01.002]
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侗医药松杖方对类风湿关节炎患者滑膜细胞STAT3炎症信号表达的影响

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年1期
页码:
8-11
栏目:
基础研究
出版日期:
2021-01-05

文章信息/Info

Title:
Effect of Dong medicine Usnea & Polygonum cuspidatum formula on STAT3 inflammation signal expression in synovial cells of patients with rheumatoid arthritis
作者:
安 阳1; 马武开1; 黄 颖1; 曹跃朋1; 宁乔怡1; 刘 静2; 杨庆万1; 项飞燕1
（1.贵州中医药大学第二附属医院,贵州 贵阳 550003; 2.贵州省遵义市第一人民医院,贵州 遵义 563000）
Author(s):
AN Yang; MA Wukai; HUANG Ying; CAO Yuepeng; NING Qiaoyi; LIU Jing; YANG Qingwan; XIANG Feiyan
（The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550003,China）
关键词:
类风湿关节炎;  滑膜细胞;  松杖方;  侗医药;  转录激活因子3;  细胞因子信号转导抑制分子3
Keywords:
Rheumatoid arthritis;  Synovial cells;  Usnea &;  Polygonum cuspidatum formula;  Dong medicine;  Singal transducers and activators of transcriprion 3;  Suppressor of cytokine signaling-3
分类号:
R 593.22
DOI:
DOI:10.3969/j.issn.1000-7369.2020.01.002
文献标志码:
A
摘要:
目的:探讨松杖方对RA患者滑膜细胞(RAFS)凋亡及STAT3相关蛋白ROR-γt、SOCS-3、pSTAT3表达的影响。方法:取对数生长期RAFS,分为空白对照组、雷公藤多苷组、来氟米特组、松杖方高、中、低剂量组共六组,分别给以相应含药血清或空白血清干预24 h后,观察细胞形态,流式细胞术分析RAFS的凋亡情况,免疫印迹法检测ROR-γt、SOCS-3、pSTAT3的表达情况。结果:空白组滑膜细胞持续增殖,各给药组细胞不同程度变形及凋亡。与雷公藤多苷组、来氟米特组比较,松杖方中剂量组RAFS凋亡率相似,松杖方高剂量组凋亡率最高,差异有统计学意义(P<0.05)。与空白对照组比较,各含药血清干预组RAFS中ROR-γt、pSTAT3的表达量均下降(P<0.05),松杖方高剂量组ROR-γt、pSTAT3的表达量最低,与雷公藤多苷组及来氟米特组比较差异有统计学意义(P<0.05)。各组RAFS中SOCS-3的表达量差异未见统计学意义(P>0.05)。结论:松杖方可抑制ROR-γt、pSTAT3的基因表达,抑制炎症信号的传导,促进RAFS凋亡,阻断RA滑膜炎症持续发生。
Abstract:
Objective:To investigate the effects of Usnea & Polygonum cuspidatum Formula(UPF)on the apoptosis of synovial cells(RAFS)and the expression of STAT3 related proteins ROR-γt,SOCS-3 and pSTAT3 in RA patients.Methods:RAFS was divided into 6 groups:blank control,tripterygium glycosides,leflunomide,and UPF high,medium and low dose,These cells were in the logarithmic growth phase.After 24 h intervention with corresponding medicated serum or blank serum,the cell morphology was observed by an inverted microscope,apoptosis was analyzed by flow cytometry,and the expression of ROR-γt,SOCS-3,and pSTAT3 was detected by western blotting.Results:Synovial cells in the blank group continued to proliferate,and the morphological deformation and apoptosis of the cells in each treatment group increased to different degrees.The apoptotic rate of the middle-dose group of UPF was similar to that of the tripterygium glycoside group and the leflunomide group.The high-dose group of UPF was higher than these three groups and was statistically significant(P<0.05).The expression levels of ROR-γt and pSTAT3 in RAFS in each intervention group were lower than those in the blank control group(P<0.05).The expression of ROR-γt and pSTAT3 in the high-dose UPF group was the lowest,which was significantly different from the tripterygium glycosides and leflunomide group(P<0.05).There was no significant difference in the expression of SOCS-3 in each group(P>0.05).Conclusion:UPF be related to decreased expression of ROR-γt and pSTAT3 protein,poor inflammatory signal conduction,and increased RAFS apoptosis may,which may cause the continuous occurrence of RA synovial inflammation and be cut off.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81960909); 贵阳市科技局基金资助项目(筑科合同[2017-4] 23 号); 贵州省高层次创新型人才培养计划-“百”层次人才项目(黔科合平台人才[2016] 5650); 贵州省中医药(民族医药)治疗风湿病医学平台建设项目(黔科合平台人才[2018] 5707)
更新日期/Last Update: 2021-01-11