[1]何 婷,刘晓芳.基于细胞介导的免疫应答观察逐水饮治疗肺癌胸腔积液疗效及机制探究[J].陕西中医,2021,(3):288-292.[doi:DOI:10.3969/j.issn.1000-7369.2020.03.004]
　HE Ting,LIU Xiaofang.Study on the effect and mechanism of Zhushuiyin in the treatment of lung cancer pleural effusion based on cell mediated immune response[J].,2021,(3):288-292.[doi:DOI:10.3969/j.issn.1000-7369.2020.03.004]
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基于细胞介导的免疫应答观察逐水饮治疗肺癌胸腔积液疗效及机制探究

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年3期
页码:
288-292
栏目:
基础研究
出版日期:
2021-03-09

文章信息/Info

Title:
Study on the effect and mechanism of Zhushuiyin in the treatment of lung cancer pleural effusion based on cell mediated immune response
作者:
何 婷1; 刘晓芳2
(1.大连市中医医院,辽宁 大连 116013; 2.黑龙江中医药大学,黑龙江 哈尔滨 150040)
Author(s):
HE Ting; LIU Xiaofang
(Dalian Hospital of Traditional Chinese Medicine,Dalian 116013,China)
关键词:
免疫应答;  逐水饮;  肺癌;  胸腔积液;  恶性;  细胞介导
Keywords:
Immune response;  Zhushuiyin;  Lung cancer;  Pleural effusion;  Malignant;  Cell mediated
分类号:
R 273
DOI:
DOI:10.3969/j.issn.1000-7369.2020.03.004
文献标志码:
A
摘要:
目的:基于细胞介导的免疫应答观察逐水饮治疗小鼠Lewis肺癌胸腔积液模型的疗效并探究其作用机制。 方法:85只C57BL/6小鼠随机分为空白参照组、模型组、逐水饮组、顺铂组、逐水饮联合顺铂组,每组各17只。空白参照组予腹腔注射氯化钠溶液,其余各组小鼠腹腔注射Lewis肺癌细胞悬液来构建小鼠模型,造模第4 天开始进行药物干预,连续干预10 d,收集并记录MPE体积、胸壁转移瘤个数以及生存天数; 测定外周血Th17、Treg细胞数量、TGF-β1以及CTLA-4含量。结果:药物干预后,与模型组比较,其余三组的MPE减少、胸壁转移瘤个数减少、生存天数延长,差异有统计学意义(均P<0.05)。各组Th17数量高于空白参照组,差异具有统计学意义(均P<0.05)。逐水饮联合顺铂组的Th17细胞数量高于逐水饮组、顺铂组,差异具有统计学意义(均P<0.05)。各组Treg细胞数量均高于空白参照组,差异具有统计学意义(均P<0.05),逐水饮联合顺铂组的Treg细胞数量低于逐水饮组、顺铂组,差异具有统计学意义(均P<0.05)。各组的TGF-β1水平较空白参照组均有升高,逐水饮组、顺铂组、逐水饮联合顺铂组的TGF-β1水平与模型组比较降低,差异具有统计学意义(均P<0.05)。各组的CTLA-4水平较空白参照组有升高,逐水饮组、顺铂组、逐水饮联合顺铂组的CTLA-4水平与模型组比较均降低,差异具有统计学意义(均P<0.05)。结论:逐水饮联合化疗药物可能通过调整Th17/Treg免疫失衡状态,纠正免疫缺陷,改善微环境免疫抑制情况以此达到减少MPE聚集的作用。
Abstract:
Objective:Based on the cell-mediated immune response,the therapeutic effect of Zhushuiyin on lewis lung cancer model with pleural effusion was observed and its mechanism explored.Methods:85 C57BL/6 mice were randomly divided into blank reference group,model group,Zhushuiyin group,cisplatin group,Zhushuiyin combined with cisplatin group,with 17 mice in each group.The blank reference group was intraperitoneally injected with sodium chloride solution,and mice in the remaining groups were injected with Lewis lung cancer cell suspension to construct mouse model.Drug intervention was started on the 4th day of modeling,and the intervention was continued for 10 days.MPE volume and chest wall were collected and recorded.The number of metastases and the number of survival days,the number of Th17 ,Treg cells,TGF-β1 and CTLA-4 content in peripheral blood were determined.Results:After drug intervention,compared with the model group,the other three groups had statistical significance in the reduction of MPE,the number of chest wall metastases,and the prolongation of survival days(all P<0.05).The number of Th17 in each group was higher than that of the blank reference group,the difference was statistically significant(all P<0.05).The number of Th17 cells in the Zhushuiyin combined with cisplatin group was significantly higher than that of the control group.The drinking group,the cisplatin group,the difference was statistically significant(all P<0.05).The number of Treg cells in each group was higher than the blank reference group,the difference was statistically significant(all P<0.05).The number of Treg cells in the Zhushuiyin combined with cisplatin group was significantly lower than that in the Zhushuiyin group and the cisplatin group.The difference was statistically significant(all P<0.05).The level of TGF-β1 in each group was higher than that of the blank reference group.The TGF-β1 levels of Zhushuiyin group,cisplatin group,and Zhushuiyin combined with cisplatin group were all lower than the model group,statistically significant(all P<0.05).The levels of CTLA-4 in each group were higher than those in the blank reference group.The levels of CTLA-4 in the Zhushuiyin group,cisplatin group,and Zhushuiyin combined with cisplatin group were all lower than the model group.It was statistically significant(all P<0.05).Conclusion:The traditional Chinese medicine prescription of Zhushuiyin contract chemotherapy drugs may reduce the aggregation of MPE by adjusting the immune imbalance of Th17/Treg,correcting the immune deficiency and improving the immunosuppression of microenvironment.

参考文献/References:

[1] Sahn SA.Management of malignant pleural effusion[J].Monaldi Arch Chest Dis,2001,56(5):394-399.
[2] 宋 娟,刘 丹,李 镭,等.肺癌伴发胸腔积液的临床特征及生存特点[J].华西医学,2018,33(1):36-40.
[3] Semaan R,Feller KD,Slatore C,et al.Malignant pleural effusions[J].Am J Respir Crit Care Med,2016,194(6):11-12.
[4] Egan AM,Mcphillips D,Sarkar S,et al.Malignant pleural effusion[J].Clinics in Chest Medicine,2018,39(1):179-184.
[5] 龙 婧,郑 琪,马婕群,等.恶性胸腔积液治疗的研究进展[J].医学综述,2019,25(3):470-474.
[6] 李秋荐,耿 良.葶苈大枣泻肺汤合苓桂术甘汤加减治疗晚期肺癌合并恶性胸腔积液临床研究[J].陕西中医,2018,39(3):289-291.
[7] Theodora A,Anastasios D,Giannou AC,et al.Mutant kras promotes malignant pleural effusion formation[J].Nat Commun,2017,8(1):2383-2389.
[8] 周恺骅,高基民.小鼠肺癌正位模型的建立[J].浙江农业科学,2018,59(12):2323-2328.
[9] 王 霞,许 瑶,许尤琪,等.泽漆汤联合顺铂胸腔灌注治疗恶性胸腔积液临床研究[J].陕西中医,2018,39(3):286-288.
[10] 李金金,轩伟霞,张晓菊.恶性胸腔积液产生的机制研究进展[J].国际呼吸杂志,2018,38(10):783-786.
[11] 邹 华,单锦露,李梦侠,等.438例肺癌恶性胸腔积液的诊治及预后因素分析[J].重庆医学,2015,44(27):3794-3797.
[12] 宋 娟,刘 丹,李 镭,等.肺癌伴发胸腔积液的临床特征及生存特点[J].华西医学,2018,33(1):36-40.
[13] 郭 欢.成人胸腔积液的临床特点分析[J].世界最新医学信息文摘,2017,17(28):91,94.
[14] 金志超,施 展,花宝金.中医药防治恶性胸腔积液的研究评述[J].中华中医药杂志,2017,32(8):3339-3341.
[15] 王贝贝.从《金匮要略》浅析癌性胸腔积液的证治[J].中医临床研究,2019,11(4):43-45.
[16] 朱菊香,杨 毅.杨毅辨证治疗恶性胸腔积液的经验[J].世界最新医学信息文摘,2018,18(28):186,189.
[17] 李俊娇,葛信国.葛信国教授运用扶正逐饮法治疗肺癌恶性胸腔积液经验[J].中国中医急症,2018,27(1):159-161.
[18] 毛文丽,李竹英.刘建秋教授辨治恶性胸腔积液经验[J].吉林中医药,2015,35(6):564-566.
[19] 李 政,王 巍,李 康.葶苈甘遂逐水饮联合胸腔内灌注化疗治疗晚期非小细胞肺癌伴恶性胸水临床疗效分析[J].辽宁中医药大学学报,2016,18(1):198-200.
[20] Ye ZJ,Zhou Q,Gu YY,et al.Correction:Generation and differentiation of IL-17-producing CD4+T cells in malignant pleural effusion[J].Journal Immunol,2014,193(9):474-478.
[21] Wang T,Lv M,Qian Q,et al.Increased frequencies of T helper type 17 cells in tuberculous pleural effusion[J].Tuberculous,2011,91(3):231-237.
[22] 王 兵,关江锋,颜琳琳,等.消水方联合顺铂对肺癌移植瘤胸腔积液小鼠模型Th17/Treg免疫平衡的影响[J].中国实验方剂学杂志,2018,24(21):131-137.
[23] Zhou L,Lopes JE,Chong MW,et al.TGF-β-induced foxp 3 inhibits Th17 cell differentiation by antagonizing ror gammat function[J].Nature,2008,453(7192):236-240.
[24] 李 慧,刘 岩,柳 影,等.CTLA-4、PD-1和PD-L1在小细胞肺癌外周血中的分布及临床意义[J].中国肺癌杂志,2017,20(11):755-760.
[25] 周立云,余小红,谢兆兰.扶正逐水饮与香菇多糖联合吡柔比星对恶性胸水患者免疫功能的影响[J].辽宁中医杂志,2012,39(11):2209-2210.

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备注/Memo

备注/Memo:
基金项目:黑龙江省中医药科研项目(ZHY16-022)
更新日期/Last Update: 2021-03-08