[1]刘 丹,胥 旸,邢晓静.鸦胆子苦醇对三阴性乳腺癌细胞凋亡的影响[J].陕西中医,2021,(4):419-422.[doi:DOI:10.3969/j.issn.1000-7369.2020.04.003]
　LIU Dan,XU Yang,XING XiaoJing.Effects of BRU on apoptosis of triple negative breast cancer cells[J].,2021,(4):419-422.[doi:DOI:10.3969/j.issn.1000-7369.2020.04.003]
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鸦胆子苦醇对三阴性乳腺癌细胞凋亡的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年4期
页码:
419-422
栏目:
基础研究
出版日期:
2021-04-05

文章信息/Info

Title:
Effects of BRU on apoptosis of triple negative breast cancer cells
作者:
刘 丹1; 胥 旸1; 邢晓静2
(1.辽宁中医药大学,辽宁 沈阳110167; 2.辽宁省肿瘤医院血液乳腺内科,辽宁 沈阳110042)
Author(s):
LIU Dan; XU Yang; XING XiaoJing
(Liaoning University of Traditional Chinese Medicine,Shenyang 110167,China)
关键词:
三阴性乳腺癌;  MDA-MB-231细胞;  鸦胆子苦醇;  细胞凋亡;  细胞增殖;  细胞周期
Keywords:
Triple negative breast cancer;  MDA-MB-231 cells;  Brusatol;  Cell apoptosis;  Cell proliferation;  Cell cycle
分类号:
R 737.9
DOI:
DOI:10.3969/j.issn.1000-7369.2020.04.003
文献标志码:
A
摘要:
目的: 研究鸦胆子苦醇(BRU)对三阴性乳腺癌MDA-MB-231细胞的凋亡影响。方法:用BRU 处理 MDA-MB-231 细胞。采用 CCK-8 法检测MDA-MB-231细胞生长抑制率,流式细胞仪检测细胞的凋亡率和周期。结果:BRU对三阴性乳腺癌MDA-MB-231细胞生长抑制率测定结果显示,不同浓度的BRU对MDA-MB-231细胞生长抑制率与对照组相比存在明显差异,均具有统计学意义(均P<0.05),且呈时间与浓度依赖性,凋亡结果显示,经5、10、20、40 μmol/L BRU作用细胞48 h后,随着浓度增高细胞的凋亡率也相应提高,与空白对照组相比差异均有统计学意义(均P<0.05)。周期结果提示经5、10、20、40 μmol/L BRU作用细胞48 h后,随药物浓度增加,处于G0/G1期的细胞比例逐渐增加,S/G2/M期的细胞比例下降,与空白对照组相比差异均有统计学意义(均P<0.05)。结论:中药鸦胆子苦醇在体外能抑制三阴性乳腺癌MDA-MB-231细胞的生长,并能诱导该细胞凋亡。
Abstract:
Objective:To study the effect of BRU on apoptosis of MDA-MB-231 cells in triple negative breast cancer.Methods:MDA-MB-231 cells were treated with BRU.CCK-8 method was used to detect the growth inhibition rate of MDA-MB-231 cells.The apoptosis rate and cell cycle were detected by flow cytometry.Results:The results of the determination of growth inhibition rate of MDA-MB-231 cells in triple negative breast cancer by BRU showed that the growth inhibition rate of MDA-MB-231 cells in different concentrations of BRU was significantly different from that in the control group(all P<0.05),which was time-dependent and concentration dependent.Apoptosis results showed that the cells were treated with 5,10,20 and 40 μmol/L BRU for 48 hours,the apoptosis rate of the cells increased with the increase of the concentration,and the differences were statistically significant compared with the blank control group(all P<0.05).Cell cycle results suggested that the cells were treated with 5,10,20,40 μmol/L BRU for 48 h,the proportion of cells in G0/G1phase gradually increased with the increase of drug concentration,and the proportion of cells in S/G2/M phase decreased,with statistically significant differences compared with the blank control group(all P<0.05).Conclusion:Brusatol can inhibit the growth of MDA-MB-231 cells in triple negative breast cancer and induce their apoptosis in vitro.

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备注/Memo

备注/Memo:
基金项目:辽宁省自然科学基金资助项目(2015020254)
更新日期/Last Update: 2021-04-09