[1]房如意,谢道俊.化痰祛瘀方在肝豆状核变性模型小鼠海马神经细胞增殖中的作用及相关机制研究[J].陕西中医,2021,(5):557-560.[doi:DOI:10.3969/j.issn.1000-7369.2020.05.003]
　FANG Ruyi,XIE Daojun.Effect and mechanism of Huatan Quyu recipe on proliferation of hippocampal neurons in mice with Wilson disease[J].,2021,(5):557-560.[doi:DOI:10.3969/j.issn.1000-7369.2020.05.003]
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化痰祛瘀方在肝豆状核变性模型小鼠海马神经细胞增殖中的作用及相关机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年5期
页码:
557-560
栏目:
基础研究
出版日期:
2021-05-05

文章信息/Info

Title:
Effect and mechanism of Huatan Quyu recipe on proliferation of hippocampal neurons in mice with Wilson disease
作者:
房如意1; 谢道俊2
(1.安徽中医药大学,安徽 合肥 230038; 2.安徽中医药大学第一附属医院,安徽 合肥 230031)
Author(s):
FANG Ruyi; XIE Daojun
(Anhui University of Traditional Chinese Medicine,Hefei 230038,China)
关键词:
肝豆状核变性;  化痰祛瘀方;  小鼠模型;  海马神经细胞;  细胞增殖;  PI3K-AKT-mTOR;  磷酸化水平
Keywords:
Wilson disease;  Huatan Quyu recipe;  Mouse model;  Hippocampal neurons;  Cell proliferation;  PI3K-AKT-mTOR;  Phosphorylation level
分类号:
R 277.7
DOI:
DOI:10.3969/j.issn.1000-7369.2020.05.003
文献标志码:
A
摘要:
目的:探讨化痰祛瘀方在肝豆状核变性(Wilson病)模型小鼠海马神经细胞增殖中的作用及PI3K-AKT-mTOR信号通路相关机制。方法:准备健康4月龄Wilson病模型TX小鼠和DL小鼠。制备中药化痰祛瘀方溶液备用。分组设立正常对照组、模型组和实验组,按实验设计分组处理。采用Western blot检测PI3K-AKT-mTOR信号通路相关因子和增殖相关因子的蛋白表达水平; 同时,采用Brd U掺入法检测化痰祛瘀方处理Wilson病模型小鼠海马神经细胞增殖。结果:与正常对照组相比,模型组总PI3K、AKT和mTOR及p-PI3K、p-AKT和p-mTOR蛋白水平均明显上调,而PCNA和Ki-67蛋白表达水平及Brd U阳性细胞数明显降低(均P<0.05)。同时,与模型组相比,实验组总PI3K、AKT和mTOR蛋白水平及p-PI3K、p-AKT和p-mTOR均明显降低,而PCNA和Ki-67蛋白表达水平和Brd U阳性细胞数明显增加(均P<0.05)。结论:化痰祛瘀方可有助于促进Wilson病模型小鼠海马神经细胞增殖,增加PCNA和Ki-67蛋白表达水平,其可能与抑制PI3K-AKT-mTOR信号通路激活,抑制PI3K、AKT和mTOR磷酸化水平相关。
Abstract:
Objective:To investigate the effect of Huatan Quyu recipe on the proliferation and invasion ability of breast cancer MCF-7 cell line and its impact on the expression level of microRNA-100(miR-100).Methods:Healthy 4-month-old TX mice and DL mice were purchased for experiments.Huatan Quyu recipe solution was prepared for subsequent usage.The normal control group,model group and experimental group were set up.Western blot was used to detect the protein expression levels of factors related to PI3K-AKT-mTOR signaling pathway and cell proliferation; meanwhile,Brd U incorporation method was used to detect the proliferation of hippocampal nerve cells in Wilson disease model mice treated with Huatan Quyu recipe.Results:Compared with the normal control group,the protein expressions of total PI3K,Akt and mTOR,p-PI3K,p-Akt and p-mTOR were significantly upregulated,while those of PCNA and Ki-67 as well as the number of Brd U positive cells were reduced obviously in the model group(all P<0.05).At the same time,compared with the model group,the protein expression levels of total PI3K,Akt and mTOR,p-PI3K,p-AKT and p-mTOR were evidently decreased,while those of PCNA and Ki-67 as well as the number of Brd U positive cells were highly increased(all P<0.05).Conclusion:Huatan Quyu recipe can promote the proliferation of hippocampal neurons and increase the expression levels of PCNA and Ki-67 protein in Wilson disease model mice,which may be related to the inhibited activation of PI3K-Akt-mTOR signaling pathway and the suppressed phosphorylation levels of PI3K,Akt and mTOR.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81874389)
更新日期/Last Update: 2021-05-10