[1]任耀龙,杨 磊,赵明君,等.丹参饮预处理保护缺血/再灌注损伤模型大鼠心肌细胞实验研究[J].陕西中医,2021,(6):692-695.[doi:DOI:10.3969/j.issn.1000-7369.2020.06.003]
　REN Yaolong,YANG Lei,ZHAO Mingjun,et al.Experimental study on protective effect of Danshen decoction pretreatment on myocardial cells in ischemia/reperfusion injury model rats[J].,2021,(6):692-695.[doi:DOI:10.3969/j.issn.1000-7369.2020.06.003]
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丹参饮预处理保护缺血/再灌注损伤模型大鼠心肌细胞实验研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年6期
页码:
692-695
栏目:
基础研究
出版日期:
2021-06-05

文章信息/Info

Title:
Experimental study on protective effect of Danshen decoction pretreatment on myocardial cells in ischemia/reperfusion injury model rats
作者:
任耀龙1; 杨 磊1; 赵明君1; 郑 刚2; 徐佳萌2; 尤金枝1; 李莹超1
(1.陕西中医药大学附属医院,陕西 咸阳 712021; 2.陕西中医药大学,陕西 咸阳 712046)
Author(s):
REN Yaolong; YANG Lei; ZHAO Mingjun; ZHENG Gang; XU Jiameng; YOU Jinzhi; LI Yingchao
(Affiliated Hospital of Shaanxi Traditional Chinese Medicine University,Xianyang 712021,China)
关键词:
心肌梗死;  丹参饮;  缺血/再灌注损伤;  心肌酶;  心肌组织学;  大鼠
Keywords:
Myocardial infarction;  Danshen decoction;  Ischemia/reperfusion injury;  Myocardial enzymes;  Myocardial histology;  Rats
分类号:
R 289
DOI:
DOI:10.3969/j.issn.1000-7369.2020.06.003
文献标志码:
A
摘要:
目的:研究丹参饮预处理对心肌缺血/再灌注损伤大鼠心肌细胞的保护作用。方法:将50只SD大鼠随机分为假手术组、模型组、丹参饮低剂量组、丹参饮中剂量组、丹参饮高剂量组,每组10只,采用前降支结扎法制备大鼠心肌缺血/再灌注损伤模型。观察干预后各组大鼠心电图、心肌酶标志物、N-末端脑钠肽前体(NT-proBNP)变化情况; 应用TTC染色法测定心肌梗死面积、HE染色观察心肌病理学改变。结果:与模型组比较,丹参饮预处理的各组能降低大鼠血清肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)与NT-proBNP水平,缩小心肌梗死面积(均P<0.01),改善心肌病理学形态,且随丹参饮浓度增加,以上作用更为显著。结论:丹参饮预处理可明显降低缺血/再灌注损伤大鼠血清心肌酶学水平,缩小心肌梗死面积,减轻心肌细胞缺血/再灌注损伤,保护心功能,且存在剂量依赖关系。
Abstract:
Objective:To study the protective effect of Danshen decoction pretreatment on myocardial cells in rats with myocardial ischemia/reperfusion injury.Methods:50 SD rats were randomly divided into sham operation group,model group,Danshen decoction low-dose group,Danshen decoction middle-dose group and Danshen decoction high-dose group,10 mice in each group.Rat model of myocardial ischemia/reperfusion injury by anterior descending branch ligation,observe electrocardiogram,myocardial zymogram and(N-terminal probrain natriuretic peptide,NT-proBNP),determination of myocardial infarction area by TTC staining,HE staining to observe myocardial pathological changes.Results:Compared with the model group,the levels of CK-MB,LDH and NT-proBNP in Danshen decoction pretreatment group were significantly reduced,myocardial infarction area were reduced(all P<0.05),myocardial pathology morphology improvement,and as the concentration of Danshen decoction increases.The above effects were more significant.Conclusion:Danshen decoction pretreatment can significantly reduce serum myocardial enzyme levels in rats with ischemia/reperfusion injury,reduce area of myocardial infarction,protect cardiomyocytes from ischemia/reperfusion injury,protective cardiac function,and has dose relationship.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(30973830); 陕西省重大病种中医药创新计划项目[陕财办(2015)99号]; 陕西省教育厅科研资助项目(19JK0236); 陕西省中医药管理局科研资助项目(2019-GJ-LC019); 陕西中医药大学附属医院吴以岭院士专家工作站资助项目[陕组通字(2019)49号]
更新日期/Last Update: 2021-06-09