[1]董宏丹.黄芩苷对结肠癌HT-29细胞移植瘤小鼠IL-6/STAT3信号转导通路的影响[J].陕西中医,2021,(6):696-700.[doi:DOI:10.3969/j.issn.1000-7369.2020.06.004]
　DONG Hongdan.Effect of baicalin on IL-6/STAT3 signal transduction pathway in mice with carcinoma of colon HT-29 cell transplantation tumor[J].,2021,(6):696-700.[doi:DOI:10.3969/j.issn.1000-7369.2020.06.004]
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黄芩苷对结肠癌HT-29细胞移植瘤小鼠IL-6/STAT3信号转导通路的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年6期
页码:
696-700
栏目:
基础研究
出版日期:
2021-06-05

文章信息/Info

Title:
Effect of baicalin on IL-6/STAT3 signal transduction pathway in mice with carcinoma of colon HT-29 cell transplantation tumor
作者:
董宏丹
(中国医科大学附属盛京医院,辽宁 沈阳 110022)
Author(s):
DONG Hongdan
(Shengjing Hospital of China Medical University,Shenyang 110022,China)
关键词:
黄芩苷;  结肠癌;  抑瘤率;  细胞凋亡;  炎症因子;  白细胞介素-6/信号转导及转录活化因子-3
Keywords:
Baicalin;  Carcinoma of colon;  Tumor inhibition rate;  Apoptosis;  Inflammatory factor;  Interleukin-6/signal transcription activating factor-3
分类号:
R 735.3
DOI:
DOI:10.3969/j.issn.1000-7369.2020.06.004
文献标志码:
A
摘要:
目的:探讨黄芩苷对结肠癌HT-29细胞移植瘤小鼠IL-6/STAT3信号转导通路的影响。方法:选取SPF级健康雄性昆明种小鼠96只,采用人结肠癌HT-29细胞悬液右前肢腋窝处皮下注射建立结肠癌移植瘤模型。将小鼠随机分为对照组、模型组、黄芩苷低剂量组、黄芩苷中剂量组、黄芩苷高剂量组及阳性对照组,各16只,均腹腔注射给药10 ml/kg、1次/d,连续14 d。检测比较各组肿瘤体积、肿瘤质量、抑瘤率,肿瘤组织细胞凋亡指数(AI),血清IL-6、IL-10水平,以及肿瘤组织IL-6、STAT3、p-STAT3、Bcl-2蛋白表达水平。结果:与模型组比较,各组小鼠抑瘤率、肿瘤组织AI较高,且黄芩苷低剂量组<黄芩苷中剂量组<黄芩苷高剂量组<阳性对照组,差异有统计学意义(均P<0.05)。与模型组比较,各组小鼠肿瘤体积和质量、血清IL-6、IL-10水平、肿瘤组织IL-6、p-STAT3、Bcl-2蛋白表达水平较低,且黄芩苷低剂量组>黄芩苷中剂量组>黄芩苷高剂量组>阳性对照组,差异有统计学意义(均P<0.05)。结论:黄芩苷能剂量依赖性的抑制结肠癌HT-29细胞移植瘤小鼠肿瘤生长,促进肿瘤细胞凋亡,其作用可能与抑制IL-6/STAT3信号转导通路活性有关。
Abstract:
Objective:To analysis the effect of baicalin on IL-6/STAT3 signal transduction pathway in mice with carcinoma of colon HT-29 cell transplantation tumor.Methods:96 clean healthy male Kunming mice were selected,and established colon carcinoma transplanted by subcutaneous injection of human carcinoma of colon HT-29 cell suspension into the axilla of right forelimb.The mice were randomly divided into control group model group baicalin low-dose group baicalin medium-dose group baicalin high-dose group,and positive control group,16 mice in each group,all mice were intraperitoneally injected with 10 ml/kg once a day for 14 d.The tumor volume and quality,tumor suppressor rate,apoptotic index(AI)of tumor tissues,serum levels of IL-6 IL-10,and expression of IL-6,STAT3,p-STAT3,Bcl-2 protein in tumor tissues were detected and compared.Results:Compared with model group,the tumor suppressor rate and AI of tumor tissues were higher,and baicalin low-dose group < baicalin middle-dose group < baicalin high-dose group < positive control group.The differences were statistically significant(all P<0.05).Compared with the model control group,the tumor volume and quality,serum levels of IL-6,IL-10,and expression of IL-6,p-STAT3,Bcl-2 in tumor tissues were lower in each group,and baicalin low-dose group > baicalin middle-dose group > baicalin high-dose group > positive control group.The differences were statistically significant(all P<0.05).Conclusions:Baicalin can inhibit the growth of carcinoma of colon HT-29 cell transplantation tumor in dose-dependent manner,promotes apoptosis of tumor tissue,and the effect may be related to the inhibition of IL-6/STAT3 signal transduction pathway activity.

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备注/Memo

备注/Memo:
基金项目:国家重点研发计划项目(2017YFC1702605,2017YFC1702606)
更新日期/Last Update: 2021-06-09