[1]陈燕伟,马善波,权 伟,等.柴胡皂苷D对慢性应激诱导小鼠焦虑症的影响[J].陕西中医,2021,(6):705-708.[doi:DOI:10.3969/j.issn.1000-7369.2020.06.006]
　CHEN Yanwei,MA Shanbo,QUAN Wei,et al.Effect of saikosaponin D on anxiety disorder induced by chronic stress in mice[J].,2021,(6):705-708.[doi:DOI:10.3969/j.issn.1000-7369.2020.06.006]
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柴胡皂苷D对慢性应激诱导小鼠焦虑症的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年6期
页码:
705-708
栏目:
基础研究
出版日期:
2021-06-05

文章信息/Info

Title:
Effect of saikosaponin D on anxiety disorder induced by chronic stress in mice
作者:
陈燕伟1; 马善波1; 权 伟2; 张 蕊1
(1.空军军医大学西京医院,陕西 西安 710032; 2.西安市精神卫生中心,陕西 西安 710199)
Author(s):
CHEN Yanwei; MA Shanbo; QUAN Wei; ZHANG Rui
(Xijing Hospital,Air Military Medical University,Xi'an 710032,China)
关键词:
柴胡;  柴胡皂苷D;  慢性应激;  焦虑症;  受体相互作用蛋白140
Keywords:
Bupleurum;  Saikosaponin D;  Chronic stres;  Anxiety disorder;  Receptor-interacting protein 140
分类号:
R 749.7
DOI:
DOI:10.3969/j.issn.1000-7369.2020.06.006
文献标志码:
A
摘要:
目的:探讨柴胡皂苷D对慢性应激诱导焦虑模型小鼠的影响及其作用机制。方法:选取50只雄性ICR小鼠,按照随机数字表法分为五组,分别为正常组、慢性应激组、氟西汀组(20 mg/kg)、柴胡皂苷D低剂量组(20 mg/kg)和柴胡皂苷D高剂量组(40 mg/kg)。除正常组外,其余四组予慢性应激诱导小鼠建立焦虑症模型。通过蔗糖偏好实验、旷场试验、强迫游泳试验和悬尾试验检测小鼠行为学。采用酶联免疫技术检测IL-6、IL-β、TNF-α; 采用Western blot技术检测小鼠海马组织RIP140、p-NF-κB-p65、p-IκBα、p-IKKα和p-IKKβ蛋白表达; 采用免疫组化法检测小鼠海马组织RIP140蛋白表达。结果:柴胡皂苷D低剂量组、柴胡皂苷D高剂量组均能改善焦虑症模型小鼠行为学,并能降低血清IL-6、IL-β和TNF-α水平。柴胡皂苷D低剂量组、柴胡皂苷D高剂量组可降低小鼠海马组织RIP140、p-NF-κB-p65、p-IκBα、p-IKKα和p-IKKβ蛋白的表达。结论:柴胡皂苷D可以缓解慢性应激焦虑模型小鼠症状,其机制与调控RIP140/NF-κB信号通路相关。
Abstract:
Objective:To explore the effect of saikosaponin D on mice of chronic stress induced anxiety model and its mechanism.Methods:Selected 50 male ICR mice were randomly divided into five groups:normal group,chronic stress group,fluoxetine group(20 mg/kg),saikosaponin D low dose group(20 mg/kg)and saikosaponin D high dose group(40 mg/kg).In addition to the normal group,the other four groups were given chronic stress induced mice to establish anxiety disorder model.The behavior of mice was tested by sucrose preference test,open field test,forced swimming test and tail suspension test.Enzyme-linked immunosorbent assay was used to detect serum IL-6,IL-β and TNF-α.Western blot technology was used to detect the expression of RIP140,p-NF-κB-p65,p-IκBα,p-IKKα and p-IKKβ protein in the hippocampus of mice.Immunohistochemical method was used to detect the expression of RIP140 protein.Results:Saikosaponin D low dose group and saikosaponin D high dose group can improve the behavior of mice with anxiety disorder and significantly reduce the levels of IL-6,IL-β and TNF-α.Saikosaponin D low dose group and saikosaponin D high dose group can significantly reduce the expression of mouse hippocampal RIP140,p-NF-κB-p65,p-IκBα,p-IKKα and p-IKKβ protein.Conclusion:Saikosaponin D can relieve the symptoms of chronic stress anxiety model mice,and its mechanism is related to the regulation of RIP140/NF-κB signaling pathway.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基础研究计划项目(2020JM-701)
更新日期/Last Update: 2021-06-09