[1]杨 繁,曾永蕾.苦参碱对宫颈癌细胞中Bcl-2/Bax表达水平及生物学行为的影响[J].陕西中医,2021,(7):840-843.[doi:DOI:10.3969/j.issn.1000-7369.2020.07.006]
　YANG Fan,ZENG Yonglei.Effect of matrine on Bcl-2/Bax expression level and biological behavior in cervical cancer cells[J].,2021,(7):840-843.[doi:DOI:10.3969/j.issn.1000-7369.2020.07.006]
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苦参碱对宫颈癌细胞中Bcl-2/Bax表达水平及生物学行为的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年7期
页码:
840-843
栏目:
基础研究
出版日期:
2021-07-09

文章信息/Info

Title:
Effect of matrine on Bcl-2/Bax expression level and biological behavior in cervical cancer cells
作者:
杨 繁1; 曾永蕾2
(1.安徽中医药大学,安徽 合肥 230038; 2.安徽中医药大学第二附属医院,安徽 合肥 230038)
Author(s):
YANG Fan; ZENG Yonglei
(Anhui University of Chinese Medicine,Hefei 230038,China)
关键词:
宫颈癌;  Hela细胞;  苦参碱;  活性;  凋亡;  侵袭
Keywords:
Cervical cancer;  Hela cells;  Matrine;  Activity;  Apoptosis;  Invasion
分类号:
R 737.33
DOI:
DOI:10.3969/j.issn.1000-7369.2020.07.006
文献标志码:
A
摘要:
目的:探讨苦参碱对宫颈癌细胞中Bcl-2/Bax表达水平及生物学行为的影响。方法:将宫颈癌Hela细胞分为宫颈癌组和药物组,宫颈癌组无药物干预,药物组采用三种1.0、1.5、2.0 g/L浓度干预,采用MTT法、Transwell法、流式细胞仪及免疫印迹分别检测Hela细胞活性抑制率、细胞侵袭数目、细胞凋亡及细胞中Bcl-2/Bax蛋白表达。结果:MTT结果显示,宫颈癌组Hela细胞活性抑制率低于药物组(P<0.05),2 g/L药物组Hela细胞活性抑制率高于1、1.5 g/L药物组(P<0.05),同一组中,72 h Hela细胞活性抑制率高于24、48 h(P<0.05),随着药物浓度及时间延长Hela细胞活性抑制率逐渐升高; 1 g/L药物组Hela细胞侵袭数目低于宫颈癌组(P<0.05),2 g/L药物组Hela细胞侵袭数目低于1、2 g/L药物组(P<0.05),四组细胞侵袭数目比较,差异有统计学意义(P<0.05); G0-G1期宫颈癌组细胞凋亡与药物组比较,具有统计学差异(P<0.05),G0-G1期、G2-M期各组相差较小(P>0.05),2 g/L药物组Hela细胞凋亡率最高,宫颈癌组细胞凋亡率最低,四组Hela细胞凋亡率比较,差异有统计学意义(P<0.05); 1 g/L药物组Hela细胞下Bcl-2蛋白水平低于宫颈癌组(P<0.05),2 g/L药物组Hela细胞下Bcl-2蛋白水平低于1、1.5 g/L药物组(P<0.05),Bax表达与Bcl-2相反。结论:苦参碱抑制宫颈癌Hela细胞活性,加快Hela凋亡,减少Hela细胞侵袭,并具有浓度依赖性,其作用机制可能与抑制Bcl-2表达,增加Bax水平相关。
Abstract:
Objective:To investigate the effect of matrine on the expression of Bcl-2/Bax and biological behavior in cervical cancer cells.Methods:Cervical cancer Hela cells cultured in vitro were cervical cancer group and drug group.The former group had no drug intervention.The drug group was treated with three concentrations of 1.0,1.5,and 2.0 g/L.Various methods were used to detect the inhibition rate of cervical cancer Hela cell activity,the number of cell invasion,the apoptosis rate,and the Bcl-2/Bax protein expression level in the cells.Results:MTT results showed that the rate of inhibition of Hela cell activity in the cervical cancer group was lower than that in the drug group(P<0.05),and the rate of Hela cell activity inhibition of the 2 g/L drug group was higher than that of the 1 g/L and 1.5 g/L drug group(P<0.05).In the same group,the inhibition rate of Hela cell activity at 72 h was higher than that of 24 h and 48 h(P<0.05),and the inhibition rate of Hela cell activity gradually increased with the increase of drug concentration and time.The number of Hela cell invasion in the 1 g/L drug group was lower than that of the cervix cancer group(P<0.05),2 g/L drug group Hela cell invasion number was lower than 1,2g/L drug group(P<0.05),the cell invasion number of the four groups was significantly different(P<0.05).Apoptosis of G0-G1 cervical cancer group was different from that of drug group(P<0.05),G0-G1 and G2-M groups had little difference(P>0.05),Hela cell apoptosis rate of 2 g/L drug group the highest rate,the lowest rate of apoptosis in cervical cancer group,the significant difference in the apoptosis rate of Hela cells in the four groups(P<0.05).The Bcl-2 protein level in Hela cells of the 1 g/L drug group was lower than that in the cervical cancer group(P<0.05),The level of Bcl-2 protein in Hela cells in the 2 g/L drug group was lower than those in the 1 and 1.5 g/L drug group(P<0.05).Conclusion: Matrine can inhibit Hela cell activity,accelerate Hela apoptosis and reduce HeLa cell invasion in a concentration dependent manner,and its mechanism may be related to the inhibition of bcl-2 expression and the increase of Bax level.

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备注/Memo

备注/Memo:
基金项目:安徽省自然科学基金资助面上项目(1608085MH233); 安徽省十三五医疗卫生重点专科项目[皖卫教科2017(30号)]; 安徽中医药大学第二附属医院曾永蕃名中医工作室项目[中发展(2020)10号]
更新日期/Last Update: 2021-07-07