[1]董文娟,杨立宏,张文佳.降脂瘦身汤对非酒精性脂肪肝大鼠脂代谢及核转录因子-κB蛋白表达影响的实验研究[J].陕西中医,2021,(8):1016-1019.[doi:DOI:10.3969/j.issn.1000-7369.2020.08.007]
　DONG Wenjuan,YANG Lihong,ZHANG Wenjia.Study of Jiangzhi Shoushen decoction on lipid metabolism and NF-κB protein expression with nonalcoholic fatty liver rats[J].,2021,(8):1016-1019.[doi:DOI:10.3969/j.issn.1000-7369.2020.08.007]
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降脂瘦身汤对非酒精性脂肪肝大鼠脂代谢及核转录因子-κB蛋白表达影响的实验研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年8期
页码:
1016-1019
栏目:
基础研究
出版日期:
2021-08-05

文章信息/Info

Title:
Study of Jiangzhi Shoushen decoction on lipid metabolism and NF-κB protein expression with nonalcoholic fatty liver rats
作者:
董文娟; 杨立宏; 张文佳
(新疆医科大学第一附属医院,新疆 乌鲁木齐 830054)
Author(s):
DONG Wenjuan; YANG Lihong; ZHANG Wenjia
(The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)
关键词:
非酒精性脂肪肝;  降脂瘦身汤;  大鼠;  脂代谢;  肝脏组织;  NF-κB
Keywords:
Nonalcoholic fatty liver disease;  Jiangzhi Shoushen decoction;  Rats;  Lipid metabolism;  Liver tissue;  Nuclear factor-κB
分类号:
R 575.5
DOI:
DOI:10.3969/j.issn.1000-7369.2020.08.007
文献标志码:
A
摘要:
目的:研究降脂瘦身汤对非酒精性脂肪肝(NFALD)大鼠脂代谢及核转录因子-κB(NF-κB)蛋白表达的影响。方法:将32只SD大鼠随机分为正常组、模型组、降脂瘦身汤组,除正常组外,其余两组采用高脂饲料喂养8周建立非酒精性脂肪肝大鼠模型。造模成功后,降脂瘦身汤组给予相应的药物水溶液,正常组和模型组给予等量0.9%氯化钠溶液灌胃,疗程4周。称取大鼠体重、肝湿重,计算肝指数,检测甘油三酯(TG)、总胆固醇(TC)、血糖(Glu)、游离脂肪酸(FFA)、胰岛素(INS)水平,苏木素-伊红(HE)染色观察肝脏组织病理的变化; 观察NF-κB蛋白表达情况。结果:①与正常组相比,模型组大鼠体重、肝湿重、肝指数增加(均P<0.01),血清Glu、FFA、TG、TC、INS水平升高(均P<0.05); ②与模型组相比,中药降脂瘦身汤组大鼠体重、肝湿重、肝指数下降(均P<0.01),血清Glu、FFA、TG、TC、INS水平降低(均P<0.05); ③HE染色显示,模型组大鼠肝脏脂肪变性明显,降脂瘦身汤组病变轻于模型组; ④降脂瘦身汤组大鼠脂肪组织NF-κB-p65、NF-κB-p-p65蛋白水平较模型组显著降低(均P<0.05)。结论:中药降脂瘦身汤可明显降低非酒精性脂肪肝大鼠的血脂水平,改善肝脏病理损伤; 其机制可能与下调NF-κB蛋白表达相关。
Abstract:
Objective:To study the effect of Jiangzhi Shoushen decoction on lipid metabolism and nuclear factor-κB(NF-κB)protein expression with nonalcoholic fatty liver disease in rats.Methods:32 SD rats were randomly divided into normal control group,model group and Jiangzhi Shoushen decoction group.Except the normal control group,the other two groups were fed with high-fat diet for 8 weeks to establish nonalcoholic fatty liver model.After successful modeling,Jiangzhi Shoushen decoction group was given corresponding medicine solution.Normal control group and model group were given normal saline for 4 weeks.Measured the body weight and wet weight of liver,detected the liver index was calculated,the levels of TG,TC,Glu,FFA and INS,observed the pathological changes of liver tissue by HE staining,and observed the expression of NF-κB protein.Results:① Compared with the normal control group,the body weight,liver wet weight and liver index of the model group increased(all P<0.01),the Glu,FFA,TG,TC and INS levels increased(all P<0.05).②Compared with the model group,the body weight,liver wet weight and liver index of the Jiangzhi Shoushen decoction group decreased(all P<0.01),the Glu,FFA,TG,TC and INS levels decreased(all P<0.05).③He staining showed that the fatty degeneration of liver was obvious in the model group,and the pathological changes in jiangzhi Shoushen decoction group were lighter than that in model group.④The levels of NF-κB-p65 and NF-κB-p-p65 protein in fat tissue of Jiangzhi Shoushen decoction group were significantly lower than those in the model group(P<0.05).Conclusion:Jiangzhi Shoushen decoction can significantly reduced the level of blood lipid and improved the pathological damage of liver in rats with nonalcoholic fatty liver,the mechanism may be related to the down-regulation of NF-κB protein expression.

参考文献/References:

[1] Younossi Z,Henry L.Contribution of alcoholic and nonalcoholic fatty liver diseaseto the burden of liver-related morbidity and mortality[J].Gastroenterology,2016(150):1778-1785.
[2] Lallukka S.Non-alcoholic fatty liver disease and risk of type2 diabetes[J].Best Pract Res Clin Endocrinol Metab,2016(30):385-395.
[3] 王亚军,耿 辉,王晓艳,等.西安市体检人群脂肪肝患病情况及相关因素分析[J].陕西医学杂志,2017,46(6):810-812.
[4] Le KA.Subcutaneous adiposetissue macrophage infiltration is associated with hepatic and visceral fatdeposition,hyperinsulinemia,and stimulation of NF-kappa B stress pathway[J].Diabetes,2011,60(11):2802-2809.
[5] 王敏娟,王敏雯,郭 艳,等.膳食炎症指数与老年人非酒精性脂肪性肝病关系研究[J].陕西医学杂志,2020,49(12):1589-1592.
[6] Takahashi Y,Sugimoto K,Inui H,et al.Current pharmacological therapies for nonalcoholic fatty liver disease/nonalcoholic steatohepatitis[J].World J Gastroenterol,2015,21(13):3777-3785.
[7] 汤莉伟,唐东旭,哈明昊.王晓素教授治疗脂肪肝经验介绍[J].陕西中医,2018,39(9):1295-1297.
[8] Christopher PD.Non-alcoholic fatty liver disease:A massive problem[J].Clin Med,2011,11(2):176-178.
[9] Napetschnig J,Wu H.Molecular basis of NF-kappaB signaling[J].Annu Rev Biophys,2013,42:443-468.
[10] 隋晓丹,邓厚波,刘铁军.中医药防治非酒精性脂肪肝病的研究进展[J].世界华人消化杂志,2013,21(18):1708-1713.
[11] 张 慧,冯 琴,胡义扬,等.祛湿化瘀方对CCl4复合高脂低蛋白饮食诱导的大鼠脂肪肝的防治作用[J].上海中医药杂志,2006,40(3):52-55.
[12] 彭继升,杨晋翔,安 静,等.降浊化瘀合剂对2型糖尿病合并非酒精性脂肪肝大鼠NF-κB通路的作用[J].上海中医药杂志,2013,47(8):74-77.
[13] 贺倩倩,赵 峻,赵 鹏.健脾理气化浊方治疗酒精性脂肪肝疗效观察[J].陕西中医,2017,38(2):141-143.
[14] 郭建立,张睦清,韩 雪,等.丹参不同组分防治大鼠非酒精性脂肪肝模型作用机制的研究[J].河北中医药学报,2012,27(1):8-9.
[15] 杨翠荣.郁金药理及中医临床应用略述[J].光明中医,2014,29(8):1772-1773.
[16] 赵永见,牛 凯,唐德志,等.桃仁药理作用研究近况[J].辽宁中医杂志,2015,42(4):888-890.
[17] Lim DW,Kim YT,Jang YJ,et al.Anti-obesity effect of artemisia capillaris extracts in high-fat diet-induced obese rats[J].Molecules,2013,18(8):9241-9252.
[18] 王永辉,高 丽,周文静,等.决明子乙醇提取物对高脂血症模型大鼠糖脂代谢及相关炎性细胞因子的影响[J].中国实验方剂学杂志,2014,20(7):178-181.
[19] 郁相云,钟建华,张 旭.泽泻降血脂药理作用及物质基础研究[J].中国中医药现代远程教育,2010,8(11):250.
[20] 胡慧明,邵 峰,朱彦陈,等.南山楂提取物对高脂血症模型小鼠胆固醇生物合成的影响[J].中药材,2015,38(5):1034-1036.
[21] 余轶群,陈 希,李军祥.绞股蓝、生山楂水提物对非酒精性脂肪性肝炎大鼠脂质代谢相关因子的调控作用研究[J].辽宁中医药大学学报,2012,14(7):168-170. (收稿:2021-02-25)

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备注/Memo

备注/Memo:
基金项目:新疆维吾尔自治区自然科学基金资助项目(2016D01C310); 新疆医科大学临床医学院高峰学科校内配套经费资助项目(33-0104006020801)
更新日期/Last Update: 2021-08-09