[1]文小竹,谭光波,朴金安(韩国).补肺健脾益肾方对COPD模型大鼠TLR4/MyD88/NF-κB通路相关因子的影响[J].陕西中医,2021,(9):1163-1166.[doi:DOI:10.3969/j.issn.1000-7369.2020.09.003]
　WEN Xiaozhu,TAN Guangbo,PIAO Jin'an.Effect of Bufei Jianpi Yishen decoction on expression of TLR4/MyD88/NF-κB pathway related factors in COPD model rats[J].,2021,(9):1163-1166.[doi:DOI:10.3969/j.issn.1000-7369.2020.09.003]
点击复制

补肺健脾益肾方对COPD模型大鼠TLR4/MyD88/NF-κB通路相关因子的影响
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年9期
页码:
1163-1166
栏目:
基础研究
出版日期:
2021-09-05

文章信息/Info

Title:
Effect of Bufei Jianpi Yishen decoction on expression of TLR4/MyD88/NF-κB pathway related factors in COPD model rats
作者:
文小竹; 谭光波; 朴金安(韩国)
(湖南中医药大学研究生院,湖南 长沙 410208)
Author(s):
WEN Xiaozhu; TAN Guangbo; PIAO Jin'an
(Graduate School of Hunan University of Traditional Chinese Medicine,Changsha 410208,China)
关键词:
慢性阻塞性肺疾病;  补肺健脾益肾方;  Toll样受体;  髓样分化因子;  核转录因子κB;  炎症因子
Keywords:
Chronic obstructive pulmonary disease;  Bufei Jianpi Yishen decoction;  Toll like receptor;  Myeloid differentiation factor;  Nuclear transcription factor-κB;  Inflammatory factor
分类号:
R 714.253
DOI:
DOI:10.3969/j.issn.1000-7369.2020.09.003
文献标志码:
A
摘要:
目的:研究补肺健脾益肾方对大鼠慢性阻塞性肺疾病(COPD)模型Toll样受体4(TLR4)/髓样分化因子88(MyD88)/核转录因子κB(NF-κB)通路的影响。方法:采用随机数字表法将40只大鼠随机分为模型组、常规组、实验组、正常组,每组10只。取其干预12周后肺组织和支气管肺泡灌洗液,比较各组大鼠干预期间一般情况,比较各组大鼠TLR4 mRNA、MyD88 mRNA、NF-κB mRNA水平及肺组织形态学评分。结果:正常组大鼠在干预期间皮毛、活动度、饮水进食、体重增长趋势均优于其余三组,常规组及实验组上述表现均优于模型组。与正常组比较,模型组大鼠TLR4 mRNA、MyD88 mRNA、NF-κB mRNA水平均升高; 与模型组比较,常规组、实验组以上指标均低,且实验组低于常规组,差异有统计学意义(均P<0.05)。与正常组比较,模型组大鼠肺组织上皮细胞变性坏死糜烂脱落、气道管壁黏膜充血水肿、气道管壁中性粒细胞浸润评分均升高,与模型组比较,常规组、实验组以上指标均降低,且实验组低于常规组,差异有统计学意义(均P<0.05)。结论:补肺健脾益肾方可通过抑制大鼠TLR4/MyD88/NF-κB通路相关因子表达改善COPD的气道炎症反应,提高治疗效果。
Abstract:
Objective:To study effect of Bufei Jianpi Yishen decoction on inhibition of Toll like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear transcription factor-κB(NF-κ B)pathway in rats with chronic obstructive pulmonary disease(COPD).Methods:40 rats were randomly divided into model group,routine group,experimental group and normal group with 10 rats in each group.The lung tissue and bronchoalveolar lavage fluid were taken after 12 weeks of intervention.The general conditions of rats in each group during intervention were compared.Levels of TLR4 mRNA,MyD88 mRNA,NF-κB mRNA and lung histomorphological score of rats in each group were compared.Results:During intervention period,fur,activity,drinking and eating,weight growth trend of the normal group were better than those of other three groups.The above performance of the routine group and the experimental group were better than that of the model group.Compared with the normal group,the TLR4 mRNA,MyD88 mRNA,NF-κB mRNA level in the model group increased significantly.The above indicators in the experimental group and the conventional group were lower than the model group,difference statistically significant(all P<0.05).Compared with the normal group,erosion of pulmonary epithelial cell necrosis,congestion and oedema of airway tube wall,and neutrophil infiltration scores of rats in the model group were significantly increased.The erosion of pulmonary epithelial cell necrosis,congestion and oedema of the airway tube wall,and neutrophil infiltration scores in the experimental group and the conventional group were lower than the model group,difference statistically significant(all P<0.05).Conclusion:Bufei Jianpi Yishen decoction can reduce airway inflammation induced by COPD and improve therapeutic effect by inhibiting expression of TLR4/MyD 88/NF-κB pathway related factors.

参考文献/References:

[1] Singh S,Verma SK,Kumar S,et al.Evaluation of oxidative stress and antioxidant status in chronic obstructive pulmonary disease[J].Scandinavian Journal of Immunology,2017,85(2):130-137.
[2] Negi H,Sarkar M,Raval AD,et al.Presence of depression & its risk factors in patients with chronic obstructive pulmonary disease[J].The Indian Journal of Medical Research,2019,139(3):402-408.
[3] Duffy SP,Criner GJ.Chronic obstructive pulmonary diseas:Evaluation and management[J].Medical Clinics of North America,2019,103(3):453-461.
[4] 刘亚萍,董 蕾,史海涛,等.LPS通过TLR4/MyD88/NF-κB途径促进人肝癌细胞增殖及转移能力[J].现代消化及介入诊疗,2019,24(3):12-16.
[5] 毛 静,王丽丽,李 亚,等.补肺健脾方对COPD大鼠骨骼肌TNF-α、Caspase-8及Caspase-3的影响[J].中华中医药杂志,2018,33(6):2373-2377.
[6] 周 凤,李德富,袁 良,等.两种不同方法建立的小鼠慢性阻塞性肺疾病模型的比较研究[J].中华结核和呼吸杂志,2019,42(5):367-371.
[7] 王 宣,王丽娜,杨 学,等.补阳还五汤对肺纤维化大鼠血清HYP含量、肺系数及肺部病理形态学的影响[J].时珍国医国药,2017,28(7):1555-1558.
[8] 周佳佳,林 呼,古 威.清金化痰汤合麻杏石甘汤治疗COPD急性加重期痰热郁肺证型临床观察[J].陕西中医,2017,38(11):1532-1533.
[9] 陈辅萍.慢性阻塞性肺疾病并发感染性肺炎危险因素分析[J].陕西医学杂志,2020,49(1):102-104.
[10] 刘宏祥,赵永辰,王 涛,等.从三焦水运失司论治慢性阻塞性肺疾病[J].中华中医药杂志,2018,33(3):955-957.
[11] 李国栋,牛 洁,吴志松,等.近20年中药治疗慢性阻塞性肺疾病稳定期组方用药规律文献研究[J].中医杂志,2019,60(7):79-83.
[12] Laurien G,Rachid A,Hoogslag GE,et al.Left ventricular global longitudinal strain and long-term prognosis in patients with chronic obstructive pulmonary disease after acute myocardial infarction[J].European Heart Journal Cardiovascular Imaging,2019,20(1):56-65.
[13] 董 斐,柳红良,谷晓红.基于“肺-三焦膜原-肾”理论对新型冠状病毒肺炎肺肾同损的认识与思考[J].北京中医药大学学报,2020,43(5):357-362.
[14] 黄海英,康俊丽,余亚辉,等.基于UPLC-Q-Orbitrap MS法分析补肺益肾方的化学成分[J].分析测试学报,2019,38(1):1-13.
[15] 王靓雅,刘 冰,祖 权,等.补肺健脾益肾方治疗稳定期慢性阻塞性肺疾病营养状况和对免疫功能的影响[J].中国实验方剂学杂志,2017,23(22):182-187.
[16] 杨丽霞,贾钦尧,陈 艳,等.Toll样受体2,4/MyD88/NF-κB信号通路及相关分子在COPD发病机制中的作用及临床意义[J].西部医学,2020,32(12):1811-1815.
[17] 李 超,宋 伟,彭 冰,等.养阴清热化瘀方对急性放射性肺炎大鼠TLR-4/NF-κB信号通路的影响[J].陕西中医,2020,41(7):864-867.
[18] 秦燕勤,董浩然,李建生,等.补肺益肾方对单核细胞条件培养基刺激下肺泡上皮细胞炎症反应的影响[J].中国中西医结合急救杂志,2017,24(1):63-67.
[19] 宋明利,巨芳萍.COPD患者肌肉抑制素、HCT、炎症细胞因子水平变化及其与肺功能的关系[J].中国急救复苏与灾害医学杂志,2019,14(12):1187-1190.
[20] 熊新军,熊龙军.COPD 稳定期患者接受健脾补肾汤后 PARC/CCL-18含量,气道重塑程度以及 MAPKs 通路功能的评估[J].海南医学院学报,2016,22(17):1954-1957.

相似文献/References:

[1]杨栓柱,王 文 △,李 猛,等.千金苇茎汤合瓜蒌薤白杏仁汤治疗 慢性阻塞性肺疾病急性加重期临床研究*[J].陕西中医,2019,(5):600.
[2]王 敏,陈 阁.清热化痰祛瘀方联合西药治疗慢性阻塞性肺疾病急性 加重期患者的临床研究[J].陕西中医,2019,(5):610.
　WANG Min,CHEN Ge..Clinical study of Qingre Huatan Quyu prescription combined with western medicine in the treatment of acute exacerbation of chronic obstructive pulmonary disease[J].,2019,(9):610.
[3]李天浩,韩晨旭,王惠琴,等.基于EGFR-MUC5AC信号通路探讨参鱼化痰口服液干预慢性阻塞性肺疾病大鼠气道黏液高分泌的机制*[J].陕西中医,2019,(8):995.
　LI Tianhao,HAN Chenxu,WANG Huiqin,et al.Based on EGFR-MUC5AC signaling pathway to explore the mechanism of Shenyu Huatan oral liquid in intervention of airway mucus high secretion in COPD rats[J].,2019,(9):995.
[4]王珊珊,谭光波△.补肺汤对慢性阻塞性肺疾病肺气肿大鼠血清IFNγ、IL4表达水平影响研究*[J].陕西中医,2019,(11):1491.
　WANG Shanshan,TAN Guangbo..Effect of Bufei decoction on the expression of IFNγ and IL4 in serum of rats with chronic obstructive pulmonary disease and emphysema[J].,2019,(9):1491.
[5]任英杰,王 起,陈秋生.黄芪汤加减治疗急性加重期慢性阻塞性肺疾病疗效研究[J].陕西中医,2020,(1):57.
　REN Yingjie,WANG Qi,CHEN Qiusheng..Observation on the curative effect of Huangqi decoction in the treatment ofacute exacerbation of chronic obstructive pulmonary disease[J].,2020,(9):57.
[6]聂清华,范伏元△.射干麻黄汤联合沙丁胺醇对慢性阻塞性肺疾病患者肺功能、生活质量的影响[J].陕西中医,2020,(2):180.
　NIE Qinghua,FAN Fuyuan..Effects of Shegan Mahuang decoction and albuterol on pulmonary function and quality of life in patients with COPD[J].,2020,(9):180.
[7]刘新平,王展儒.理肺化痰通脉方治疗慢性阻塞性肺疾病急性加重期临床研究[J].陕西中医,2020,(4):484.
[8]杜沂岚,唐 敏,陈朝晖△,等.经肺腧穴中药离子导入联合振动排痰治疗慢性阻塞性肺疾病急性加重期临床疗效*[J].陕西中医,2020,(4):534.
　DU Yilan,TANG Min,CHEN Zhaohui,et al.Clinical efficacy of Chinese medicine iontophoresis via Feishu point combined with vibration sputum discarding in the treatment of AECOPD[J].,2020,(9):534.
[9]唐颖丽,谢 珊,余清萍,等.子午流注法治疗慢性阻塞性肺疾病加重期痰浊阻肺证疗效研究*[J].陕西中医,2020,(9):1311.[doi:DOI:10.3969/j.issn.1000-7369.2020.09.039]
　TANG Yingli,XIE Shan,YU Qingping,et al.Curative effect of midnight-noon ebb-flow method on chronic obstructive pulmonary disease exacerbation with syndrome of Tanzhuo Zufei[J].,2020,(9):1311.[doi:DOI:10.3969/j.issn.1000-7369.2020.09.039]
[10]蔡东林,孙钟和,赵益庆.慢性阻塞性肺疾病稳定期BODE指数、IL-8、TNF-α水平与肺肾气虚证相关性研究*[J].陕西中医,2020,(11):1573.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.015]

备注/Memo

备注/Memo:
基金项目:湖南省教育厅科学研究项目(19C1412)
更新日期/Last Update: 2021-09-10