[1]周 雪.基于Bcl-2/Bax/Caspase-3信号通路探讨三七总皂苷对自身免疫性心肌炎模型大鼠心肌凋亡影响的研究[J].陕西中医,2021,(11):1504-1510.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.003]
　ZHOU Xue.Effects of panax notoginseng saponins on myocardial apoptosis in rats with experimental autoimmune myocarditis based on Bcl-2/Bax/Caspase-3 signaling pathway[J].,2021,(11):1504-1510.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.003]
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基于Bcl-2/Bax/Caspase-3信号通路探讨三七总皂苷对自身免疫性心肌炎模型大鼠心肌凋亡影响的研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年11期
页码:
1504-1510
栏目:
基础研究
出版日期:
2021-11-05

文章信息/Info

Title:
Effects of panax notoginseng saponins on myocardial apoptosis in rats with experimental autoimmune myocarditis based on Bcl-2/Bax/Caspase-3 signaling pathway
作者:
周 雪
(沈阳医学院附属第二医院,辽宁 沈阳 110000)
Author(s):
ZHOU Xue
(The Second Affiliated Hospital of Shenyang Medical College,Shenyang 110000,China)
关键词:
自身免疫性心肌炎;  三七总皂苷;  心功能;  细胞凋亡;  B淋巴细胞瘤-2
Keywords:
Experimental autoimmune myocarditis;  Panax notoginseng saponins;  Cardiac function;  Apoptosis;  Bcl-2
分类号:
R 542.21
DOI:
DOI:10.3969/j.issn.1000-7369.2020.11.003
文献标志码:
A
摘要:
目的:探讨三七总皂苷通过B淋巴细胞瘤-2(Bcl-2)/ Bcl-2相关x蛋白(Bax)/含半胱氨酸的天冬氨酸蛋白水解酶-3(Caspase-3)信号通路对自身免疫性心肌炎(EAM)模型大鼠心肌凋亡的影响。方法:55只Lewis大鼠被分成对照组(n=11)和造模组(n=44)。造模组采用猪心肌肌球蛋白乳浊液后足垫皮下注射建立EAM模型,对照组大鼠给予等体积完全弗氏佐剂后足垫皮下注射。成功建立EAM模型的大鼠被分成模型组、三七总皂苷低、高剂量组和甲氨蝶呤组各11只。模型组、对照组通过灌胃给予蒸馏水10 ml/kg,每日1次; 三七总皂苷低、高剂量组分别灌胃给予10、20 mg/ml三七总皂苷溶液10 ml/kg,每日1次; 甲氨蝶呤组灌胃给予0.1 mg/ml甲氨蝶呤混悬液10 ml/kg,每周1次。各组大鼠均给药4周。结果:与模型组比较,三七总皂苷低、高剂量组和甲氨蝶呤组大鼠左室射血分数(LVEF)和左室短轴缩短率(LVFS)较高,而左室舒张末期内径(LVIDd)、左室收缩末期内径(LVIDs)水平较低,心肌组织病理评分较低,心肌组织细胞凋亡率较低,心肌组织Bcl-2表达水平较高,Bax、Caspase-3表达水平较低(均P<0.05),且三七总皂苷干预组呈现一定量效关系。结论:三七总皂苷能剂量依赖性的改善EAM大鼠心功能,减轻心肌炎症病理变化,抑制细胞凋亡,其作用可能与调节Bcl-2/Bax/Caspase-3信号通路中凋亡相关蛋白表达有关。
Abstract:
Objective:To analysis the effect of panax notoginseng saponins on myocardial apoptosis in rats with experimental autoimmune myocarditis(EAM)based on B-lymphoma-2(Bcl-2)/Bcl-2-related x protein(Bax)/cysteine containing aspartate protein hydrolase-3(Caspase-3)signaling pathway.Methods:Fifty-five Lewis rats were divided into control group(n=11)and model building group(n=44).The model building group was established EAM model by subcutaneous injection of porcine myosin emulsion into the foot pad,rats in the control group received subcutaneous injection of equal volume complete Freund's adjuvant into the foot pad.After the model was established,the rats in model building group were randomly divided into model group,panax notoginseng saponins low-dose,high-dose group and methotrexate group,each with 11 rats.The model group and control group were given distilled water 10 ml/kg respectively by gavage,once a day; panax notoginseng saponins low-dose,high-dose group were given 10,20 mg/ml panax notoginseng saponins solution 10 ml/kg respectively by gavage,once a day; methotrexate group was given methotrexate suspension 10 ml/kg by gavage,once a week.All rats in each group were treated for 4 weeks.Results:Compared with model group,left ventricular ejection fraction(LVEF)and left ventricular short axis shortening rate(LVFS)were higher,the levels of left ventricular end diastolic diameter(LVIDd)and left ventricular end systolic diameter(LVIDs)were lower,the pathological scores of myocardial tissue were lower,the apoptosis rates of myocardial tissue were lower, the expression levels of Bcl-2 in myocardium were higher,and the expression levels of Bax and Caspase-3 were lower in panax notoginseng saponins low-dose,high-dose group and methotrexate group(all P<0.05),and panax notoginseng saponins intervention group showed a certain dose effect relationship.Conclusion:Panax notoginseng saponins can can improve the cardiac function of EAM rats in a dose-dependent manner,alleviate the pathological changes of myocarditis and inhibit apoptosis,which may be related to the regulation of apoptosis related protein expression in Bcl-2/Bax/Caspase-3 signaling pathway.

参考文献/References:

[1] Zainal Z,Rahim AA,Radhakrishnan AK,et al.Investigation of the curative effects of palm vitamin E tocotrienols on autoimmune arthritis disease in vivo[J].Scientific Reports,2019,9(1):16793.
[2] Yj A,Jj A,Jkc B,et al.Functional annotation of noncoding causal variants in autoimmune diseases[J].Genomics,2020,112(2):1208-1213.
[3] Li Y,Yang M,Liu Z,et al.Detection and diagnosis of myocarditis in young patients using ECG analysis based on artificial neural networks[J].Computing,2020,102(1):1-18.
[4] 李 亮,王 梅,刘 涛,等.扩张型心肌病的免疫治疗研究进展[J].临床心血管病杂志,2017,33(11):1031-1035.
[5] 魏思敏.自身免疫性心肌炎发病机制的研究[J].临床医药文献电子杂志,2020,7(30):19.
[6] 闫红梅,李 覃,周 欣,等.不同免疫方案建立实验性自身免疫性心肌炎小鼠模型的分析与评价[J].中西医结合心脑血管病杂志,2017,15(21):2684-2689.
[7] 王丽君,霍建华,张卫萍,等.姜黄素通过抑制心肌组织炎症反应改善自身免疫性心肌炎大鼠的心功能[J].山西医科大学学报,2018,49(8):5-9.
[8] 孙彬栩,李小江,蔡启亮,等.新加生脉饮对阿霉素所致心肌细胞凋亡及Bax、Bcl-2、Caspase-3蛋白表达的影响[J].中草药,2020,51(2):433-438.
[9] 吴志超,孙 萍,孔维忠,等.UPLC测定三七总皂苷微丸中5种皂苷类成分含量研究[J].辽宁中医药大学学报,2020,22(5):33-36.
[10] Mcnally EM,Mestroni L.Dilated cardiomyopathy[J].Circulation Research,2017,121(7):731-748.
[11] Yan F,Sheng Q,Tong L,et al.Effects of hypericum perforatum extract on the immunogenic myocardial fibrosis in mice and its mechanis[J].Chinese Journal of Applied Physiology,2019,35(5):402-405.
[12] 霍瑞民,李炜莉.三七总皂苷分期治疗高血压性脑出血及对血肿、高敏C反应蛋白的影响[J].陕西中医,2017,38(10):1370-1372.
[13] 孙 建,刘新宇.三七总皂苷胶囊治疗糖尿病周围神经病变临床疗效及机制研究[J].陕西医学杂志,2020,49(11):1130-1132.
[14] 谢 鹏,刘金海,耿胜男,等.三七总皂苷对过氧化氢所致内皮细胞损伤的保护作用[J].亚太传统医药,2020,16(9):39-43.
[15] 倪道斌,吕宏祥,陈 蓉,等.吉西他滨对小鼠实验性自身免疫性心肌炎的影响[J].江苏大学学报:医学版,2019,29(2):117-122.
[16] 丁 涛,刘 博,王 鑫,等.组分中药KX组合物及单味组分对实验性自身免疫性心肌炎模型的影响[J].中华中医药杂志,2017,32(8):392-394.
[17] Kishi H,Yamaguchi K,Watanabe K,et al.Deficiency of phospholipase A2 receptor exacerbates autoimmune myocarditis in mice[J].Inflammation,2020,43(3):1097-1109.
[18] 李舒承,王 虹,黄贤胜,等.三七总皂苷对慢性心力衰竭大鼠心脏功能、血浆利钠肽及血管紧张素Ⅱ的影响[J].东南大学学报:医学版,2019,38(4):638-642.
[19] 陈慧勤.三七总皂苷与银杏提取物及两者联合对心血管保护作用的研究进展[J].宁德师范学院学报:自然科学版,2019,31(3):69-74.
[20] 林 瑶,赵 京,石 琳,等.小儿感染性心肌炎107例临床特征分析[J].中国临床医生杂志,2019,47(2):114-116.
[21] 梁润琼.含三七总皂苷注射剂不良反应分析及影响因素研究[J].健康必读,2020, 28(4):53-54.
[22] 郭向峰,王春梅,谷涌泉,等.三七总皂苷预防深静脉血栓的药理研究进展[J].江苏中医药, 2019, 51(7):90-93.
[23] 谢淑芸,蒋靖波.左卡尼汀联合环磷腺苷对病毒性心肌炎患者的疗效及对IFN-γ和IL-4水平的影响[J].心血管康复医学杂志, 2019, 28(4):96-99.
[24] 刘晓丹,丁 煌,李艳玲,等. 黄芪甲苷配伍三七总皂苷对OGD/R大鼠脑微血管内皮细胞增殖、凋亡及线粒体功能的影响[J].湖南中医药大学学报,2021,41(4):498-503.
[25] 随蓓蓓,李华舜,孟 晓,等. 三七总皂苷对人乳腺癌MDA-MB-231细胞凋亡的影响[J]. 济宁医学院学报,2021,44(2):77-79,83.
[26] 陈伟达,石 玮,杨继国,等. 三七总皂苷经miR-24靶向C-myc对骨关节炎模型大鼠软骨细胞凋亡与增殖的影响[J].浙江中西医结合杂志,2021,31(4):319-323.
[27] 李 泉,王占聚,孟 洁,等. 不同浓度三七总皂苷对人淋巴瘤Raji细胞系增殖、凋亡的影响及其机制探讨[J].山东医药,2020,60(3):9-12.
[28] 丘穗珊,薛莲芳,李康宪,等. 三七总皂苷对脑出血大鼠神经细胞凋亡及相关基因的影响[J].暨南大学学报:自然科学与医学版,2017,38(5):387-392.
[29] 李玉云,翟玮玮,杨向荣,等. 三七总皂苷对K562细胞增殖、凋亡及周期的影响及机制[J].南方医科大学学报,2015,35(8):1103-1109.

备注/Memo

备注/Memo:
基金项目:辽宁省科学事业公益研究基金资助项目(2016003006)
更新日期/Last Update: 2021-11-10