[1]郭 璐,赵兵刚,冯 婷,等.生肌散抑制RIP1/RIP3/MLKL通路介导大鼠压疮形成实验研究[J].陕西中医,2021,(11):1517-1521.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.005]
　GUO Lu,ZHAO Binggang,FENG Ting,et al.Experimental study of Shengji powder on inhibiting the formation of pressure sore in rats mediated by RIP1/RIP3/MLKL pathway[J].,2021,(11):1517-1521.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.005]
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生肌散抑制RIP1/RIP3/MLKL通路介导大鼠压疮形成实验研究

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年11期
页码:
1517-1521
栏目:
基础研究
出版日期:
2021-11-05

文章信息/Info

Title:
Experimental study of Shengji powder on inhibiting the formation of pressure sore in rats mediated by RIP1/RIP3/MLKL pathway
作者:
郭 璐; 赵兵刚; 冯 婷; 刘 沛
(空军军医大学西京医院急诊科,陕西 西安 710032)
Author(s):
GUO Lu; ZHAO Binggang; FENG Ting; LIU Pei
(Department of Emergency,Xijing Hospital,Air Force Medical University,Xi'an 710032,China)
关键词:
压疮;  生肌散;  抑制;  RIP1/RIP3/MLKL通路;  大鼠;  实验研究
Keywords:
Pressure sores;  Shengji powder;  Inhibit;  RIP1/RIP3/MLKL pathway;  Rats;  Experimental study
分类号:
R 619
DOI:
DOI:10.3969/j.issn.1000-7369.2020.11.005
文献标志码:
A
摘要:
目的:探讨生肌散在治疗大鼠压疮中的相关作用机制。方法:将60只SPF雄性SD大鼠随机分成对照组、模型组、治疗组,每组各20只。模型组制作Ⅲ期大鼠压疮模型,成模后,创面外敷0.9%氯化钠溶液纱布,2次/d,共7 d; 对照组仅同部位埋入磁铁不予磁性加压,其余处理同模型组; 治疗组成模后,创面外敷生肌散2 mg,2次/d,共7 d,其余处理同模型组。创面处理7 d后,比较各组大鼠局部皮肤组织病理学改变、受体相互作用蛋白激酶-1(RIP1)、受体相互作用蛋白激酶-3(RIP3)和混合系列激酶样结构域(MLKL)的表达、脂质过氧化产物丙二醛(MDA)、炎症介质白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量的差异。结果:肉眼及光镜观察可见,治疗组大鼠压疮组织病理学改变均较模型组明显改善; 与对照组比较,模型组RIP1、RIP3和MLKL表达、MDA及IL-1β、TNF-α含量均明显增高(均P<0.05); 与模型组比较,治疗组RIP1、RIP3和MLKL表达、MDA及IL-1β、TNF-α含量均明显减少(均P<0.05)。结论:生肌散可通过抑制RIP1/RIP3/MLKL通路介导的程序性细胞坏死,减轻脂质过氧化与炎症反应的发生发展,促进大鼠压疮创面的愈合。
Abstract:
Objective:To explore the relevant mechanism of Shengji powder in the treatment of pressure ulcers in rats.Methods:60 SPF male SD rats were randomly divided into control group,model group and treatment group(n=20).Model group:stage Ⅲ rat pressure ulcer model was made.After the model was established,normal saline gauze was applied to the wound surface twice a day for 7 days.Control group:only the same part of the magnet was embedded without magnetic pressure,and the rest was treated the same as the model group.Treatment group:after the model is formed,2 mg Shengji powder is applied on the wound surface twice a day for 7 days,and the rest is treated the same as the model group.After 7 days of wound treatment,compare the histopathological changes of local skin tissues,protein expression of receptor-interacting protein kinase1(RIP1),receptor-interacting protein kinase3(RIP3)and mixed lineage kinase domain-like protein(MLKL),lipid peroxidation product malondialdehyde(MDA),inflammatory mediator Interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)difference content in each group. Results:Macroscopic and light microscope observations showed that the histopathological changes of pressure ulcers in the treatment group were significantly improved compared with the model group.Compared with the control group,the expression of RIP1,RIP3,MLKL,MDA,IL-1β,and TNF-α levels in the model group were all significantly improved.Significantly higher(all P<0.05); compared with the model group,the expression of RIP1,RIP3,MLKL,MDA,IL-1β,and TNF-α levels in the treatment group were significantly reduced(all P<0.05).Conclusion:RIP1/RIP3/MLKL pathway plays an important role in the formation of pressure ulcers in rats.Shengji powder can inhibit RIP1/RIP3/MLKL pathway-mediated programmed cell necrosis,reduce lipid peroxidation and inflammation,and promote healing of pressure sore wounds in rats.

参考文献/References:

[1] Wang Y,Zhao M,He S,et al.Necroptosis regulates tumor repopulation after radiotherapy via RIP1/RIP3/MLKL/JNK/IL8 pathway[J].J Exp Clin Cancer Res,2019,38(1):461.
[2] Shrestha A,Mehdizadeh GI.RIP1,RIP3,and MLKL contribute to cell death caused by clostridium perfringens enterotoxin[J].mBio,2019,10(6):2985-2919.
[3] 高秉钧.疡科心得集[M].北京:人民卫生出版社,2006:1-15.
[4] 孙晓丽,王华丽,青 华.老年晚期胃癌患者发生医院获得性压疮现状及危险因素调查研究[J].陕西医学杂志,2020,49(8):1049-1052.
[5] 庄鹏晖,刘亮亮,郑国玺,等.支气管哮喘患者血清肿瘤坏死因子-α、白细胞介素-8和嗜酸性粒细胞阳离子蛋白水平变化及意义[J].陕西医学杂志,2020,49(12):1597-1599,1603.
[6] Istvan S,Zhang RY,Phillip O,et al.Development of a simple,noninvasive,clinically relevan t model of pressure ulcers in the mouse[J].J Invest Surg,2004(17):221-227.
[7] 孙之中,易倩透.脓饮联合象皮生肌膏治疗慢性皮肤溃疡疗效研究[J].陕西中医,2021,42(3):341-343.
[8] Kimura M,Shibahara N,Hikiami H,et al.Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats[J].Evid Based Complement Atternat Med,2011(11):592791.
[9] 王晓慧.血管再生在Ⅲ期压疮中的作用及外源性bFGF保护作用[D].温州:温州医科大学,2016.
[10] 姜丽萍.压疮临床分期及相关机制研究进展[J].创伤外科杂志,2012,14(2):97-99.
[11] Sharma HS,Duncan JA,Johanson CE.Whole-body hyperthermia in the rat disrupts the blood-cerebrospinal fluid barrier and induces brain edema[J].Acta Neurochir Suppl,2006(96):426-431.
[12] Hu S,Chang X,Zhu H,et al.PI3K mediates tumor necrosis factor induced-necroptosis through initiating RIP1-RIP3-MLKL signaling pathway activation[J].Cytokine,2020(129):155046.
[13] Hanson B.Necroptosis:A new way of dying?[J].Cancer Biol Ther,2016,17(9):899-910.
[14] Zhe WS,Li SG,Yue CL.The role of necroptosis in cardiovascular disease[J].Front Pharmacol,2018(9):721.
[15] Szobi A,Farkasova V,Lichy M,et al.Cardioprotection of ischaemic preconditioning is associated with inhibition of translocation of MLKL within the plasma membrane[J].J Cell Mol Med,2018,22(9):4183-4196.
[16] Xin J,You D,Breslin P,et al.Sensitizing acute myeloid leukemia cells to induced differentiation by inhibiting the RIP1/RIP3 pathway[J].Leukemia,2017,31(5):1154-1165.
[17] Xiong Y,Li L,Zhang L,et al.The bromodomain protein BRD4 positively regulates necroptosis via modulating MLKL expression[J].Cell Death Differ,2019,26(10):1929-1941.
[18] 伍 磊.紫苏子汤对哮喘小鼠TNF-α、白细胞介素-8、白介素-1β表达的影响[J].陕西中医,2019,40(2):152-155.
[19] Zhang B,Cao K,Liu Z,et al.Receptor interacting protein kinase 3 promotes cisplatin-induced necroptosis in apoptosis-resistant HepG2/DDP cells[J].Neoplasma,2019,66(5):694-703.
[20] Kaiser WJ,Sridharan H,Huang C,et al.Toll-like receptor 3-mediated necrosis via TRIF,RIP3,and MLKL[J].J Biol Chem,2013,288(43):31268-31279.

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备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2019SF-281)
更新日期/Last Update: 2021-11-10