[1]高 宗,杨家应,张念平,等.半夏厚朴汤对卒中后抑郁大鼠的干预机制研究[J].陕西中医,2021,(12):1671-1675.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.004]
　GAO Zong,YANG Jiaying,ZHANG Nianping,et al.Intervention mechanism study of Banxia Houpu decoction on rats with post-stroke depression[J].,2021,(12):1671-1675.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.004]
点击复制

半夏厚朴汤对卒中后抑郁大鼠的干预机制研究

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年12期
页码:
1671-1675
栏目:
基础研究
出版日期:
2021-12-05

文章信息/Info

Title:
Intervention mechanism study of Banxia Houpu decoction on rats with post-stroke depression
作者:
高 宗1; 杨家应2; 张念平3; 苗秀明1
(1.山东中医药大学附属医院,山东 济南 250014; 2.兰陵县人民医院神经外科,山东 临沂 277700; 3.山东中医药大学实验中心,山东 济南 250355)
Author(s):
GAO Zong; YANG Jiaying; ZHANG Nianping; MIAO Xiuming
(Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China)
关键词:
脑梗死;  卒中后抑郁;  半夏厚朴汤;  旷场实验;  细胞因子
Keywords:
Cerebral infarction;  Post-stroke depression;  Banxia Houpu decoction;  Open field test;  Cell factor
分类号:
R 255.2
DOI:
DOI:10.3969/j.issn.1000-7369.2020.12.004
文献标志码:
A
摘要:
目的:观察半夏厚朴汤对卒中后抑郁(PSD)大鼠的干预机制。方法:40只SD大鼠随机分为PSD组、半夏厚朴汤低剂量组、半夏厚朴汤高剂量组、阳性组,每组10只。PSD组给予0.9%氯化钠溶液,半夏厚朴汤低剂量组、半夏厚朴汤高剂量组分别给予1.5、3.0 g/kg半夏厚朴汤,阳性组给予1.7 mg/kg氟西汀,灌胃21 d后建立卒中后抑郁模型,造模后继续给药21 d。四氮唑红染色测定脑梗死面积,治疗前及治疗1、2、3周后测定大鼠体重、水平运动得分、糖水消耗量,HE染色观察海马组织病理学改变,ELISA法测定海马组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)水平。结果:治疗3周后,半夏厚朴汤低剂量组、半夏厚朴汤高剂量组和阳性组体重高于PSD组,差异有统计学意义(均P<0.05)。半夏厚朴汤低剂量组、半夏厚朴汤高剂量组、阳性组脑梗死面积低于PSD组,差异有统计学意义(均P<0.05)。半夏厚朴汤低剂量组、半夏厚朴汤高剂量组、阳性组水平运动得分高于PSD组,差异有统计学意义(均P<0.05)。半夏厚朴汤低剂量组、半夏厚朴汤高剂量组、阳性组糖水消耗量高于PSD组,差异有统计学意义(均P<0.05)。半夏厚朴汤低剂量组、半夏厚朴汤高剂量组、阳性组TNF-α、IL-1β、IL-18低于PSD组,差异有统计学意义(均P<0.05)。结论:半夏厚朴汤可改善PSD大鼠的抑郁症状,减少梗死面积,其作用机制可能与半夏厚朴汤促进细胞因子网络达到稳态有关。
Abstract:
Objective:To investigate the interventional effect of Banxia Houpu decoction on rats with post-stroke depression(PSD)and its possible mechanism.Methods:40 SD rats were randomly divided into PSD group,Banxia Houpu low-dose group,Banxia Houpu high-dose group and positive group,10 rats in each group.The PSD group were given the same amount of normal saline,the Banxia Houpu low-dose group and Banxia Houpu high-dose group were given 1.5 g/kg and 3.0 g/kg Banxia Houpu decoction respectively,and the positive group was given 1.7 mg/kg fluoxetine.The post-stroke depression model was established 21 days after gavage,and the drug was continued for 21 days after modeling.The area of cerebral infarction was determined by staining with tetrazolium red.Body weight,horizontal movement scores,sugar water consumption were determined before treatment,and after treatment for 1,2,3 weeks.Hippocampal pathological changes were observed by HE staining,and TNF-α,IL-1β, IL-8 were determined by ELISA.Results:After 3 weeks of treatment,the weight of the Banxia Houpu low-dose group,the Banxia Houpu high-dose group and positive group were significantly higher than those of the PSD group( all P<0.05).The cerebral infarction area of the Banxia Houpu low-dose group,the Banxia Houpu high-dose group and positive group were significantly lower than those of the PSD group(all P<0.05).The Banxia Houpu low-dose group,the Banxia Houpu high-dose group and the positive group had significantly higher levels of exercise scores than the PSD group(all P<0.05).The consumption of sugar and water in the Banxia Houpu low-dose,the Banxia Houpu high-dose and the positive groups were significantly higher than those of the PSD group(all P<0.05).The levels of TNF-α,IL-1β,IL-18 in the Banxia Houpu low-dose group,the Banxia Houpu high-dose group,and the positive group were significantly lower than those in the PSD group(all P<0.05).Conclusion:Banxia Houpu decoction can improve depressive symptoms of PSD rats and reduce the infarct size.Its mechanism may be related to the promotion of cytokine network homeostasis by Banxia Houpu decoction.

参考文献/References:

[1] Villa RF,Ferrari F,Moretti A.Post-stroke depression:Mechanisms and pharmacological treatment[J].Pharmacology & Therapeutics,2018,184(1):131-144.
[2] Das J,Rajanikant GK.Post stroke depression:The sequelae of cerebral stroke[J].Neuroscience & Biobehavioral Reviews,2018,90(1):104-114.
[3] 吴春岚,王长德,陈 婡.逍遥解郁方对卒中后抑郁大鼠5-HT含量及其受体表达影响的研究[J].陕西中医,2019,40(3):275-278.
[4] Sloten TT,Sedaghat S,Carnethon MR,et al.Cerebral microvascular complications of type 2 diabetes:Stroke cognitive dysfunction and depression[J].The Lancet Diabetes & Endocrinology,2020,8(4):325-336.
[5] 李 帆,李鹏飞,高 峰,等.基于数据挖掘的中药治疗缺血性脑卒中临床用药规律分析[J].中国老年学杂志,2020,40(7):1368-1372.
[6] 王骏阳,李九如,周余蕾,等.不同动脉阻塞及阻塞时限对大鼠局灶性脑缺血模型的病理及行为学影响[J].中国临床神经科学,2015,10(2):130-137.
[7] Wang SH,Zhang ZJ,Guo YJ,et al.Anhedonia and activity deficits in rats:Impact of post-stroke depression[J].Journal of Psychopharmacology,2009,23(3):295-304.
[8] 李宁宁,郭 海,雷延飞.半夏厚朴汤加味对慢性萎缩性胃炎大鼠胃黏膜血流量及脂质过氧化损伤的影响研究[J].现代中西医结合杂志,2016,19(20):2170-2172.
[9] 许 鹏,温 杰,崔友祥,等. 电项针对颈动脉狭窄致中风先兆患者头颈部CT血管成像及血流动力学的影响[J].陕西中医,2021,42(9):1285-1288.
[10] 姜荣荣,顾嘉美.甘草附子汤加减辅助西药治疗中风后偏瘫对患者炎症因子、Klotho蛋白的影响[J].中国医药导报,2021,18(3):156-159,180.
[11] 李 彬,白辉辉.半夏厚朴汤对痰湿阻肺型慢性阻塞性肺疾病急性期肺功能及微观指标的影响[J].陕西中医,2019,40(4):466-468.
[12] 林 俊. 半夏厚朴汤配合针刺治疗卒中后假性球麻痹吞咽障碍的疗效及安全性[J].现代养生:下半月版,2021,21(8):61-63.
[13] 蒋紫云,连乐燊,钱旭胜,等. 加味半夏厚朴汤干预慢性阻塞性肺疾病急性加重期合并阻塞性睡眠呼吸暂停低通气综合征30例临床研究[J].江苏中医药,2021,53(6):34-37.
[14] Chen HH,Ning Z,Li WY,et al.Overexpression of brain-derived neurotrophic factor in the hippocampus protects against post-stroke depression[J].Neural Regeneration Research,2015,10(9):1427.
[15] Jiang H,Xiao L,Jin K,et al.Estrogen administration attenuates poststroke depression by enhancing CREB/BDNF/TrkB signaling in the rat hippocampus[J].Experimental and Therapeutic Medicine,2021,21(5):100-103.
[16] Luo L,Deng S,Jian Y,et al.Buyang Huanwu decoction ameliorates poststroke depression via promoting neurotrophic pathway mediated neuroprotection and neurogenesis[J].Evidence-based Complementray and Alternative Medicine,2017,3(8):407-426.
[17] 陈向东,郭洪波.半夏厚朴汤加味对咽异感症患者炎症因子及临床症状的改善研究[J].中国药物与临床,2018,6(8):1341-1343.
[18] 黄世敬,陈宇霞,张 颖.厚朴治疗抑郁症及抗抑郁机理探讨[J].世界中西医结合杂志,2015,9(7):1023-1026.
[19] 吕昊哲,李庆云.半夏厚朴汤对慢性应激抑郁模型大鼠脑源性神经营养因子的影响[J].中医药信息,2008,8(4):49-50.
[20] 连乐燊,蒋紫云,刘小虹,等.半夏厚朴汤对阻塞性睡眠呼吸暂停低通气综合征患者炎症反应、氧化应激、血管内皮功能障碍的影响[J].广州中医药大学学报,2020,189(9):16-20.
[21] 李 南,赵献敏,杜彩霞,等.加味半夏厚朴汤对抑郁大鼠的干预作用[J].中医学报,2018,247(12):108-111.
[22] 牛海玲,马 琳,王廉昌,等.IL-1、IL-2、IL-6、TNF-α在脑卒中后抑郁患者血清中的表达及其临床意义[J].现代医学,2016,44(1):89-91.
[23] Yi X,Zhu X,Zhou Y,et al.The combination of insulin resistance and serum interleukin-1β correlates with post-stroke depression in patients with acute ischemic stroke[J].Neuropsychiatric Disease and Treatment,2021,17(7):735-746.
[24] Kim JM,Kang HJ,Kim JW,et al.Associations of tumor necrosis factor-α and interleukin-1β levels and polymorphisms with post-stroke depression[J].Am Journal Geriatr Psychiatry,2017,25(12):1300-1308.
[25] Kang HJ,Bae KY,Kim SW,et al.Effects of interleukin-6 interleukin-18 and statin use evaluated at acute stroke on post-stroke depression during 1-year follow-up[J].Psychoneuroendocrinology,2016,72(1):156-160.
[26] Levada OA,Troyan AS.Poststroke depression biomarkers:A narrative review[J].Front Neurol,2018,9(5):577.

相似文献/References:

[1]郭 辉,裴 育,熊伟华,等.血府逐瘀汤联合阿替普酶静脉溶栓对急性脑梗死患者 神经功能及日常生活能力的影响*[J].陕西中医,2019,(2):214.
　GUO Hui,PEI Yu,XIONG Weihua,et al.Effect of Xuefu Zhuyu decoction combined with intravenous thrombolysis on the neurological function and activities of daily living in patients with acute cerebral infarction[J].,2019,(12):214.
[2]陈 晨.补阳还五汤对急性脑梗死患者血管内皮生长因子、血管生成素2及神经功能影响的研究*[J].陕西中医,2019,(1):28.
[3]王艳民.开窍活血汤对脑梗死患者血清前白蛋白、MCP1及MMP9的影响*[J].陕西中医,2019,(1):34.
[4]贾东佩,李春雷△.调神通络针刺法对脑梗死后血管性痴呆患者血清BDNF、VEGF、MMP9的影响*[J].陕西中医,2019,(1):119.
　JIA Dongpei,LI Chunlei..Effect of Tiaoshen Tongluo acupuncture in patients with vascular dementia after cerebral infarction and its effects on serum BDNF, VEGF and MMP9[J].,2019,(12):119.
[5]周志梅,买 雷,李艳红.消栓肠溶胶囊治疗脑梗死疗效及对患者NIHSS评分的影响*[J].陕西中医,2019,(6):696.
　ZHOU Zhimei,MAI Lei,LI Yanhong..Effect of Xiaoshuan enteric-coated capsule on patients with cerebral infarction and its influence on NIHSS score of patients[J].,2019,(12):696.
[6]项广宇.化痰通络方治疗短暂性脑缺血发作后脑梗死疗效及对患者氧化应激和血清相关细胞因子水平的影响*[J].陕西中医,2019,(7):864.
[7]陈 静,陈 炜,伍 媛,等.小续命汤及其组分治疗急性脑梗死研究进展*[J].陕西中医,2019,(12):1818.
[8]赵月臣,马 兰.痰瘀阻络方联合丁苯酞治疗脑梗死急性期疗效及对患者脑血流灌注及血清6-k-PGF1a、Hcy、sICAM-1水平影响*[J].陕西中医,2020,(2):174.
　ZHAO Yuechen,MA Lan..Effect of phlegm stasis blocking collateral combined with butylphthalide on cerebral blood flow perfusion and serum 6-k-PGFla,Hcy and sICAM-1 level in patients with acute cerebral infarction[J].,2020,(12):174.
[9]黄英媛,宋振华△,徐焕杰,等.补阳还五汤联合梅花针叩刺治疗脑梗死偏瘫疗效研究*[J].陕西中医,2020,(6):815.[doi:DOI:10.3969/j.issn.10007369.2020.06.033]
　HUANG Yingyuan,SONG Zhenhua,XU Huanjie,et al.Clinical effect of Buyang Huanwu decoction combined with plum flower needle tapping on cerebral infarction hemiplegia[J].,2020,(12):815.[doi:DOI:10.3969/j.issn.10007369.2020.06.033]
[10]韩 慧,赖智勇,蒲建萍,等.头穴丛刺法联合川芎嗪对大鼠脑梗死后神经元突触重建的影响*[J].陕西中医,2020,(7):860.[doi:DOI:10.3969/j.issn.10007369.2020.07.006]
　HAN Hui,LAI Zhiyong,PU Jianping,et al.Effect of scalp acupuncture combined with ligustrazine on synaptic reconstruction of neurons after cerebral infarction in rats[J].,2020,(12):860.[doi:DOI:10.3969/j.issn.10007369.2020.07.006]

备注/Memo

备注/Memo:
基金项目:山东省中医药科技发展计划项目(2019-0109)
更新日期/Last Update: 2021-12-09