[1]江 东,徐长亮,沈卫星,等.参白解毒方对溃疡性结肠炎肠黏膜上皮细胞保护作用机制研究[J].陕西中医,2022,(1):23-27.[doi:DOI:10.3969/j.issn.1000-7369.2022.01.005]
　JIANG Dong,XU Changliang,SHEN Weixing,et al.Explore protective mechanism of Shenbai Jiedu decoction on colon epithelial cells of ulcerative colitis[J].,2022,(1):23-27.[doi:DOI:10.3969/j.issn.1000-7369.2022.01.005]
点击复制

参白解毒方对溃疡性结肠炎肠黏膜上皮细胞保护作用机制研究
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年1期
页码:
23-27
栏目:
基础研究
出版日期:
2022-01-05

文章信息/Info

Title:
Explore protective mechanism of Shenbai Jiedu decoction on colon epithelial cells of ulcerative colitis
作者:
江 东1; 徐长亮2; 沈卫星2; 程海波2
(1.南京中医药大学第一临床医学院,江苏 南京 210023; 2.江苏省中医药防治肿瘤协同创新中心 国家中医药管理局名医验方评价与转化重点实验室,江苏 南京 210023)
Author(s):
JIANG Dong; XU Changliang; SHEN Weixing; CHENG Haibo
(The First Clinical College of Nanjing University of Chinese Medicine,Nanjing 210023,China)
关键词:
溃疡性结肠炎;  炎症性肠病;  癌前病变;  参白解毒方;  癌毒;  结直肠黏膜上皮细胞;  细胞周期;  细胞增殖
Keywords:
Ulcerative colitis;  Inflammatory bowel disease;  Precancerous lesions;  Shenbai Jiedu decoction;  Cancer toxin;  Colorectal mucosal epithelial cells;  Cell cycle;  Cell proliferation
分类号:
R 574.62
DOI:
DOI:10.3969/j.issn.1000-7369.2022.01.005
文献标志码:
A
摘要:
目的:研究参白解毒方(SBJDF)对溃疡性结肠炎肠黏膜上皮细胞的保护作用及机制。方法:使用葡聚糖硫酸钠(DSS)刺激人正常结直肠黏膜上皮细胞(FHC),模拟溃疡性结肠炎黏膜上皮细胞的损伤,倒置显微镜观察参白解毒方对PHC损伤模型细胞形态的影响通过MTT法检测DSS对细胞活力的影响,5-乙炔基-2'-脱氧尿苷(EdU)检测参白解毒方对细胞增殖能力的影响,流式细胞仪检测FHC细胞的周期分布变化,通过蛋白免疫印迹法检测细胞周期相关蛋白Cyclin A2、CDK1的表达。结果:DSS能够抑制FHC细胞活力,下调FHC细胞的Cyclin A2、CDK1蛋白表达,将FHC细胞周期阻滞于G0/G1期,抑制细胞增殖; 参白解毒方给药后能减少DSS细胞毒性,增加FHC细胞的Cyclin A2、CDK1蛋白表达,进而逆转DSS导致的FHC细胞G0/G1期阻滞,增加细胞活力。结论:参白解毒方可能通过维持细胞周期稳态发挥对溃疡性结肠炎肠黏膜上皮细胞的保护作用。
Abstract:
Objective:To explore the protective mechanism of Shenbai Jiedu decoction(SBJDF)on ulcerative colitis.Methods:Dextran sodium sulfate(DSS)was used to stimulate normal fetal human colon(FHC)cells to simulate the ulcerative colitis mucosal epithelial cells.After treatment with SBJDF,cell morphology was observed by inverted microscopy,The MTT assay was used to detect the effect of SBJDF on the cell viability of FHC cells,EdU was used to detect the cell proliferation ability,The cell cycle distribution changes was observed by flow cytometry,The expression levels of proteins Cyclin A2,CDK1 were determined by Western blot analysis.Results:DSS decreased viability of FHC cells,DSS induced FHC cell cycle arrest in G0/G1 phase and down-regulated the expression of Cyclin A2 and CDK1,inhibited cell proliferation.After treatment with DSS,SBJDF can inhibit the decrease of FHC cell viability induced by DSS and reverse the down-regulation of Cyclin A2 and CDK1,thereby alleviate the G0/G1 phase block of FHC cells caused by DSS.Conclusion:SBJDF may protect colorectal mucosa cells from inflammatory bowel disease by maintaining cell cycle homeostasis.

参考文献/References:

[1] Ungaro R,Mehandru S,Allen PB,et al.Ulcerative colitis[J].Lancet,2017,389(10080):1756-1770.
[2] Feuerstein JD,Moss AC,Farraye FA.Ulcerative colitis[J].Mayo Clin Proc,2019,94(7):1357-1373.
[3] Thorsteinsdottir S,Gudjonsson T,Nielsen OH,et al.Pathogenesis and biomarkers of carcinogenesis in ulcerative colitis[J].Nat Rev Gastroenterol Hepatol,2011,8(7):395-404.
[4] Coussens LM,Werb Z.Inflammation and cancer[J].Nature,2002,420(6917):860-867.
[5] Eaden JA,Abrams KR,Mayberry JF.The risk of colorectal cancer in ulcerative colitis:A meta-analysis[J].Gut,2001,48(4):526-535.
[6] Langan RC,Gotsch PB,Krafczyk MA,et al.Ulcerative colitis:Diagnosis and treatment[J].Am Fam Physician,2007,76(9):1323-1330.
[7] Ordas I,Eckmann L,Talamini M,et al.Ulcerative colitis[J].Lancet,2012,380(9853):1606-1619.
[8] Ashton JJ,Green Z,Kolimarala V,et al.Inflammatory bowel disease:Long-term therapeutic challenges[J].Expert Rev Gastroenterol Hepatol,2019,13(11):1049-1063.
[9] Lu PD,Zhao YH.Targeting NF-κB pathway for treating ulcerative colitis:Comprehensive regulatory characteristics of Chinese medicines[J].Chinese Medicine,2020,15(1):15.
[10] Shen Z,Zhou Q,Ni Y,et al.Traditional Chinese medicine for mild-to-moderate ulcerative colitis:Protocol for a network meta-analysis of randomized controlled trials[J].Medicine,2019,98(33):e16881-e16883.
[11] 何碧霞,朱延涛.中医药治疗溃疡性结肠炎研究进展[J].新中医,2020,52(13):21-23.
[12] 黎琮毅.溃疡性结肠炎的中西医治疗进展[J].湖南中医杂志,2019,35(8):158-161.
[13] 熊 波,方盛泉.溃疡性结肠炎临床治疗的研究进展[J].中国医药导刊,2020,22(3):170-173.
[14] 郑 帆,蔡 丽,邱承智,等.中医药治疗溃疡性结肠炎临床研究进展[J].亚太传统医药,2018,14(6):91-93.
[15] Ding A,Wen X.Dandelion root extract protects NCM460 colonic cells and relieves experimental mouse colitis[J].J Nat Med,2018,72(4):857-866.
[16] Chelakkot C,Ghim J,Rajasekaran N,et al.Intestinal epithelial cell-specific deletion of PLD2 alleviates dss-induced colitis by regulating occludin[J].Sci Rep,2017,7(1):1573.
[17] 蔡 竞,李 楠,朱虹丽,等.新加归肾丸通过调节mTOR信号通路促进体外培养大鼠卵巢颗粒细胞增殖[J].陕西中医,2020,41(9):1179-1183.
[18] Wood DJ,Endicott JA.Structural insights into the functional diversity of the CDK-cyclin family[J].Open biology,2018,8(9):180112.
[19] Kanakkanthara A,Jeganathan KB.Cyclin A2 is an RNA binding protein that controls Mre11 mRNA translation[J].Science,2016,353(6307):1549-1552.
[20] Honda T,Inui M.PDZRN3 protects against apoptosis in myoblasts by maintaining cyclin A2 expression[J].Scientific Reports,2020,10(1):1140-1143.

相似文献/References:

[1]陈 岚,贾 波△,邓怀涵,等.基于“逆流挽舟”法探索人参败毒散对溃疡性结肠炎模型大鼠〖JZ〗胃肠功能的影响及作用机制*[J].陕西中医,2019,(3):283.
[2]陈立冬,刘智慧,于 蕊.康复新液灌肠联合美沙拉嗪治疗溃疡性结肠炎疗效研究*[J].陕西中医,2019,(9):1187.
[3]乔峰,强文娟,高东,等.葛根芩连汤配合针刺治疗溃疡性结肠炎活动期大肠湿热证临床研究*[J].陕西中医,2019,(11):1516.
　QIAO Feng,QIANG Wenjuan,GAO Dong,et al.Gegen Qinlian decoction combined with acupuncture for ulcerative colitis with large intestine dampheat syndrome at active stage[J].,2019,(1):1516.
[4]史英,张铁△,祝斌野,等.溃结2号方治疗脾虚湿盛型溃疡性结肠炎临床研究*[J].陕西中医,2019,(11):1520.
　SHI Ying,ZHANG Tie,ZHU Binye,et al.Clinical observation on Kuijie No.2 recipe in treating ulcerative colitis of spleen deficiency and dampness[J].,2019,(1):1520.
[5]赵克学,徐 辰,戴高中,等.愈疡膏外敷联合西药治疗复发型活动期溃疡性结肠炎临床研究*[J].陕西中医,2020,(5):600.[doi:DOI:10.3969/j.issn.10007369.2020.05.013]
　ZHAO Kexue,XU Chen,DAI Gaozhong,et al.Observation on the efficacy of Yuyang plaster combined with western medicine in the treatment of recurrent active ulcerative colitis[J].,2020,(1):600.[doi:DOI:10.3969/j.issn.10007369.2020.05.013]
[6]白鹏飞,张艳君,李 敏.荆芥连翘汤加减从肺论治溃疡性结肠炎疗效及对患者肠黏膜屏障功能、免疫调节、氧化应激指标的影响*[J].陕西中医,2020,(7):897.[doi:DOI:10.3969/j.issn.10007369.2020.07.017]
　BAI Pengfei,ZHANG Yanjun,LI Min..Effect of treating ulcerative colitis with nepeta forsythia decoction and its influence on intestinal barrier function,immune regulation and oxidative stress[J].,2020,(1):897.[doi:DOI:10.3969/j.issn.10007369.2020.07.017]
[7]郭 琳,王雅婷,考延磊,等.闫雪洁运用寒热并用法治疗溃疡性结肠炎经验*[J].陕西中医,2020,(8):1148.[doi:DOI:10.3969/j.issn.1000-7369.2020.08.036]
　GUO Lin,WANG Yating,KAO Yanlei,et al.Clinical experience of YAN Xuejie in treating ulcerative colitis with the theory of combination of cold and heat[J].,2020,(1):1148.[doi:DOI:10.3969/j.issn.1000-7369.2020.08.036]
[8]梁 想,王梅青△.参苓白术散联合美沙拉嗪治疗溃疡性结肠炎疗效研究[J].陕西中医,2020,(9):1251.[doi:DOI:10.3969/j.issn.1000-7369.2020.09.021]
[9]袁子云,杜晓泉△.杜晓泉运用健脾调肠汤治疗溃疡性结肠炎脾虚湿热证经验总结*[J].陕西中医,2020,(12):1800.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.032]
[10]邹林焘,邹正华,杜学芳.清肠解毒汤治疗溃疡性结肠炎湿热内蕴证临床研究[J].陕西中医,2021,(5):597.[doi:DOI:10.3969/j.issn.1000-7369.2020.05.013]
　ZOU Lintao,ZOU Zhenghua,DU Xuefang.Qingchang Jiedu decoction in the treatment of ulcerative colitis with internal accumulation of damp-heat[J].,2021,(1):597.[doi:DOI:10.3969/j.issn.1000-7369.2020.05.013]

备注/Memo

备注/Memo:
基金项目:国家重点研发计划项目(2017YFC1700602); 江苏省高校优势学科建设工程资助项目(PAPD)
更新日期/Last Update: 2022-01-09