[1]黄丽萍,乔博灵,颜雪珍,等.基于转录组测序及韦恩分析探寻补气中药制剂发挥补气作用的靶基因及其生物学功能[J].陕西中医,2022,(1):28-32.[doi:DOI:10.3969/j.issn.1000-7369.2022.01.006]
　HUANG Liping,QIAO Boling,YAN Xuezhen,et al.Target genes and biological functions related to tonifying Qi of Chinese medicine preparation based on transcriptome RNA sequencing and Venn analysis[J].,2022,(1):28-32.[doi:DOI:10.3969/j.issn.1000-7369.2022.01.006]
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基于转录组测序及韦恩分析探寻补气中药制剂发挥补气作用的靶基因及其生物学功能
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年1期
页码:
28-32
栏目:
基础研究
出版日期:
2022-01-05

文章信息/Info

Title:
Target genes and biological functions related to tonifying Qi of Chinese medicine preparation based on transcriptome RNA sequencing and Venn analysis
作者:
黄丽萍1; 乔博灵2; 颜雪珍1; 赵 亭1; 纪昌春1; 杨 峥1; 张晓霞1; 郭正萍1; 罗苓芝1
(1.陕西省中医医院,陕西 西安 710003; 2.西北大学生命科学院,陕西 西安 710069)
Author(s):
HUANG Liping;  QIAO Boling; YAN Xuezhen;  ZhAO Ting; JI Changchun; YANG Zheng; ZHANG Xiaoxia; GUO Zhengping; LUO Lingzhi
(Shaanxi Provincial Hospital of Chinese Medicine,Xi'an 710003,China)
关键词:
补气;  益气复脉;  参芪扶正;  高通量转录组测序;  MAPK信号通路;  FKBP5
Keywords:
Tonifying qi;  Yiqi Fumai;  Shenqi Fuzheng;  RNA-Seq;  MAPK signaling pathway;  FKBP5
分类号:
R 96
DOI:
DOI:10.3969/j.issn.1000-7369.2022.01.006
文献标志码:
A
摘要:
目的:探寻益气复脉/参芪扶正注射液发挥补气作用的潜在靶基因及其生物学功能。方法:利用高通量转录组测序技术,考察气虚患者经益气复脉/参芪扶正注射液治疗后,其外周血单核细胞的差异表达基因、GO(Gene Ontotology)分析及KEGG(Kyoto encyclopedia of genes and genomes)分析,并进一步进行韦恩分析,明确2个制剂治疗后共同的差异基因及生物学功能,最后利用一组临床队列样本,通过实时定量PCR,验证并明确与补气密切相关的基因。结果:两组共有的GO条目,包括生物过程的有32条,细胞组分的有2条,分子功能的有6条,生物通路有10条。共表达差异基因有6个,包括DUSP1、DUSP2、FKBP5、F8A2、C9orf129 EGR1。临床队列样本显示,FKBP5的下调有统计学差异(P=0.001)。结论:MAPK信号通路是益气复脉和参芪扶正发挥补气作用的核心通路,FKBP5基因水平下调是它们发挥补气作用的分子标志。
Abstract:
Objective:To identify the potential target genes and biological functions related to tonifying Qi of Yiqi Fumai and Shenqi Fuzheng injection.Methods:In this study,we performed RNA-seq experiments and comparative transcriptomic analysis on pre- and post- peripheral blood mononuclear cell samples collected from Qi-deficiency patients treated with Yiqi FuMai or Shenqi Fuzheng.The significant differentially expressed genes(DEGs)for each treatment were determined; GO(Gene ontology)and KEGG analysis were assigned.Subsequently,Venn analysis was performed to identify genes and biological functions involved in both treatments.Finally,the DEGs in common were investigated in a cohort study by using real time qPCR.Results:There were common items involved in both treatments including 32 of biological process,2 of cellular component,6 of molecular function and 10 of signaling pathway.Their consistent DEGs include DUSP1,DUSP2,FKBP5,F8A2,C9orf129 and EGR1.Down-regulation on FKBP5 showed significant differences in the cohort study(P=0.001).Conclusion:MAPK signaling pathway is an action core in both treatments.Down-regulation of FKBP5 is suggested as a response bio-marker for their function on tonifying Qi.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基金资助面上项目(2020JM-672)
更新日期/Last Update: 2022-01-09