[1]白登彦,王 冠,张海军,等.基于PI3K/AKT信号通路探究芍药甘草汤对神经根型颈椎病模型大鼠Bax和Bcl-2蛋白表达的影响[J].陕西中医,2022,(1):33-37.[doi:DOI:10.3969/j.issn.1000-7369.2022.01.007]
　BAI Dengyan,WANG Guan,ZHANG Haijun,et al.Exploring the effect of Shaoyao Gancao decoction on the expression of Bax and Bcl-2 protein in a rat model of cervical spondylotic radiculopathy based on PI3K/AKT signaling pathway[J].,2022,(1):33-37.[doi:DOI:10.3969/j.issn.1000-7369.2022.01.007]
点击复制

基于PI3K/AKT信号通路探究芍药甘草汤对神经根型颈椎病模型大鼠Bax和Bcl-2蛋白表达的影响
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年1期
页码:
33-37
栏目:
基础研究
出版日期:
2022-01-05

文章信息/Info

Title:
Exploring the effect of Shaoyao Gancao decoction on the expression of Bax and Bcl-2 protein in a rat model of cervical spondylotic radiculopathy based on PI3K/AKT signaling pathway
作者:
白登彦1; 2; 王 冠1; 张海军1; 朱 涛1; 赵志鹏1; 吕立桃3
(1.甘肃省第二人民医院,甘肃 兰州 730099; 2.西北民族大学附属医院,甘肃 兰州 730015; 3.开封市人民医院骨一科,河南 开封475001)
Author(s):
BAI Dengyan; WANG Guan; ZHANG Haijun; ZHU Tao; ZHAO Zhipeng; LYU Litao
(Gansu Second People's Hospital,Lanzhou 730099,China)
关键词:
神经根型颈椎病;  芍药甘草汤;  PI3K/AKT信号通路;  磷脂酰肌醇3-激酶;  大鼠
Keywords:
Cervical spondylotic radiculopathy;  Shaoyao Gancao decoction;  PI3K/AKT signaling pathway;  Phosphoinositide 3-kinase;  Rat
分类号:
R 658.53
DOI:
DOI:10.3969/j.issn.1000-7369.2022.01.007
文献标志码:
A
摘要:
目的:探究芍药甘草汤对神经根型颈椎病(CSR)模型大鼠血清磷脂酰肌醇3-激酶(PI3K)/丝氧酸-苏氨酸蛋白激酶(AKT)的调控作用,分析芍药甘草汤对CSR大鼠Bax及Bcl-2的影响。方法:随机将90只SPF级Wistar大鼠分为空白组、模型组、对照组、芍药甘草汤治疗低、中、高剂量组六组,每组15只。除空白组外,其他组以颈部神经根腋下鱼线卡压法构建建立CSR模型大鼠,采用大鼠步态障碍行为学评分进行模型评价。造模后7 d进行干预治疗,空白组和模型组采用生理盐水灌胃,对照组采用3MA干预,芍药甘草汤低、中、高剂量治疗组分别按8、12、16 g/kg剂量进行灌胃,分别干预治疗7 d。末次给药24 h后处死大鼠,取脊髓及神经根组织,酶联免疫吸附法测定各组大鼠丝氨酸残基的底物Bax和Bcl-2的表达水平; HE染色检测各组大鼠脊髓组织病理表现; 蛋白免疫印迹法(Western blot)检测各组大鼠脊髓组织中PI3K/AKT通路蛋白的表达情况。结果:造模后14 d,除空白组外,各组大鼠步态积分增高,提示造模成功; 给药14 d后,模型组大鼠血清中Bax表达明显增高,Bcl-2表达下降,神经根组织中PI3K、p-AKT蛋白表达降低,差异有统计学意义(P<0.05)。与模型组比较,对照组和芍药甘草汤各治疗组CSR大鼠在治疗后神经根积分明显降低,而且组织中Bax表达降低,Bcl-2表达升高(P<0.05); 神经根组织中PI3K、p-AKT蛋白表达水平显著升高(P<0.05),且芍药甘草汤治疗组大鼠具有剂量依赖性(P<0.01)。结论:芍药甘草汤可有效调控CSR模型大鼠PI3K、p-AKT蛋白表达,其机制可能与芍药甘草汤能够激活CSR大鼠组织中Bax和Bcl-2等生物因子抗细胞凋亡相关。
Abstract:
Objective:To explore the effects of Shaoyao Gancao decoction on serum phosphoinositide 3-kinase(PI3K)/seroxy-threonine protein kinase(AKT)in cervical spondylotic radiculopathy(CSR)model rats,analyze the effect of Shaoyao Gancao decoction on Bax and Bcl-2 in CSR rats.Methods:Ninety SPF Wistar rats were randomly divided into a blank group,a model group,and a control group,The Shaoyao Gancao decoction treated low,medium,and high-dose groups of 6 groups,each with 15 rats.Except for the blank group,the other groups used the neck nerve root axillary fishing line compression method to establish CSR model rats,and the gait disorder behavior score of rats was used for model evaluation.Intervention treatment was performed 7 days after modeling.The blank group and the model group were given normal saline intragastric administration,and the control group was given 3MA intervention.The low,medium and high doses of Shaoyao Gancao decoction were treated at 8,12 and 16 g/kg,respectively.The rats were intragastrically administered at a dose of 16 g/kg,and the patients were treated with intervention for 7 days.Rats were sacrificed 24 hours after the last administration,spinal cord and nerve root tissues were taken,and enzyme-linked immunosorbent assay was used to determine the expression levels of Bax and Bcl-2,the substrates of serine residues in each group HE staining was used to detect the spinal cord of each group Histopathological findings.Western blot was used to detect the expression of PI3K/AKT pathway protein in the spinal cord tissues of rats in each group.Results:14 days after modeling,except for the blank group,the gait score of each group increased,indicating that the modeling was successful.14 days after the administration,the expression of Bax in the serum of the model group increased significantly,the expression of Bcl-2 decreased,and the nerve roots the expression of PI3K and p-AKT protein in the tissues decreased,and the difference was statistically significant(P<0.05).Compared with the model group,the CSR rats in the control group and each treatment group of Shaoyao Gancao decoction significantly reduced the nerve root score after treatment,and the expression of Bax in the tissue decreased,and the expression of Bcl-2 increased(P<0.05).PI3K in the nerve root tissue the expression level of p-AKT protein was significantly increased(P<0.05),and the rats in the Shaoyao Gancao decoction treatment group were dose-dependent(P<0.01).Conclusion:Shaoyao Gancao decoction can effectively regulate the expression of PI3K and p-AKT protein in CSR model rats.The mechanism may be related to that Shaoyao Gancao decoction can activate Bax and Bcl-2 in the tissues of CSR rats to resist apoptosis.

参考文献/References:

[1] 杨 峥,高 静.神经阻滞联合埋线治疗神经根型颈椎病疗效观察[J].陕西中医,2021,42(1):112-114.
[2] Chao XU,Jie LU,Ji JJ,et al.Clinical observation of DMS combined with Maitland joint loosening in the treatment of cervical spondylopathy of nerve root type[J].Chinese Manipulation & Rehabilitation Medicine,2019,20(1):173.
[3] 郝 龙,景 蓉,高东梅.葛芪二仙汤结合臂丛神经牵拉样手法及颈椎间孔阻滞治疗单侧神经根型颈椎病临床研究[J].陕西中医,2018,39(8):1051-1053.
[4] Cui J,District L,City D,et al.Effect of small weight continuous traction on nocturnal pain induced by cervical spondylopathy of nerve root type[J].World Journal of Sleep Medicine,2019,11(1):110-112.
[5] Xiao LX,Liu CS,Zhong SZ,et al.Effect of a traction exercise neck brace on cervical spondylopathy radiculopathy:A clinical study and finite element analysis[J].Evidence-based Complementary and Alternative Medicine,2021,2021(11):1-10.
[6] 雷小平,王 星.芍药甘草汤对神经根型颈椎病患者血流动力学的影响[J].陕西中医药大学学报,2021,44(2):83-86.
[7] 杨 杰.针刀配合芍药甘草汤治疗神经根型颈椎病临床观察[J].实用中医药杂志,2019,35(11):1304-1305.
[8] 朱建宗,林晓生,邱 宏,等.中药外敷联合电针治疗气虚血瘀型神经根型颈椎病疗效观察[J].陕西中医,2017,38(9):1250-1251.
[9] Liu T.Design of hospital embedded system and the treatment of cervical spondylopathy[J].Microprocessors and Microsystems,2020,81(16):1780-1794.
[10] Song ZY,Zhang ZM,Qin XG,et al.Treatment of neck type cervical spondylopathy with Zheng's jin gou diao yu(gold-hook-fishing)acupuncture method:A randomized controlled trial[J].Journal of Acupuncture and Tuina Science,2019,17(5):350-355.
[11] 朱立国,段国平,魏 戌,等.神经根型颈椎病麻木症状的临床研究概述[J].颈腰痛杂志,2015,36(1):75-77.
[12] 赵赞赞,刘庆丰,李亚男,等.芍药甘草汤药效物质基础与质量控制方法研究进展[J].中药材,2019,42(11):2730-2735.
[13] 王佐梅,肖洪彬,李雪莹,等.芍药甘草汤的药理作用研究进展[J].现代中医药,2021,41(2):22-28.
[14] 陈进城,林 荣,张圆芳,等.基于miR-146a调控NF-κB信号通路探讨芍药甘草汤治疗颈型颈椎病兔颈后肌炎症损伤的作用机制[J].康复学报,2020,30(3):206-211.
[15] 于丽华,王 宏,秦 丽,等.基于5个PI3K通路相关基因构建肺腺癌预后预测模型[J].分子诊断与治疗杂志,2021,13(9):1440-1444.
[16] 吴江林,苏海涛,梁以豪,等.芍药甘草汤联合平衡针治疗腰椎间盘突出症腰腿痛临床研究[J].新中医,2021,53(14):10-14.
[17] Shamji MF,Guha D,Paul D,et al.Systemic inflammatory and Th17 immune activation among patients treated for lumbar radiculopathy exceeds that of patients treated for persistent postoperative neuropathic pain[J].Neurosurgery,2017,81(3):537-544.
[18] 阴 涛,郑遵成,陈梦婷,等.悬吊运动疗法对神经根型颈椎病患者表面肌电频域指标影响研究[J].陕西医学杂志,2018,47(6):739-741.
[19] 刘 军,李伟伟,弓立群,等.经前路钩突切除椎间融合术治疗失稳神经根型颈椎病疗效观察[J].陕西医学杂志,2017,46(11):1578-1580.
[20] 张云霞.针灸配合推拿治疗神经根型颈椎病疗效研究[J].陕西中医,2017,38(8):1126-1127.
[21] Xz YE,Ma Y,Shen LJ,et al.Clinical observation on 187 cases of cervical spondylopathy treated by two points and three lines' akupotomye therapy[J].China Journal of Traditional Chinese Medicine and Pharmacy,2019,17(4):352-357.
[22] 尹 立,肖 衡,王 立,等.颈椎间盘切除及椎间孔减压术治疗神经根型颈椎病30例[J].陕西医学杂志,2016,45(7):846-848.

相似文献/References:

[1]杨 峥,高 静.神经阻滞联合埋线治疗神经根型颈椎病疗效观察[J].陕西中医,2021,(1):112.[doi:DOI:10.3969/j.issn.1000-7369.2020.01.031]
[2]尉迎丽,晏圣松,唐流刚,等.郑氏“颈四步”手法治疗神经根型颈椎病疗效研究[J].陕西中医,2021,(12):1779.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.034]
　YU Yingli,YAN Shengsong,TANG Liugang,et al.Study on effect of Zheng's Jing Sibu manipulation in the treatment of cervical spondylotic radiculopathy[J].,2021,(1):1779.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.034]
[3]王 肖,缪展康,林灿彬,等.“柔肝缓急”法论治膀胱过度活动症[J].陕西中医,2022,(2):219.[doi:DOI:10.3969/j.issn.1000-7369.2022.02.019]
　WANG Xiao,MIAO Zhankang,LIN Canbin,et al.Treatment of overactive bladder with “soft liver and slow and urgent”[J].,2022,(1):219.[doi:DOI:10.3969/j.issn.1000-7369.2022.02.019]
[4]徐震球,杜国庆,张明才.活血行气止痛汤联合针刀法治疗神经根型颈椎病疗效研究[J].陕西中医,2022,(9):1286.[doi:DOI:10.3969/j.issn.1000-7369.2022.09.034]
[5]房晓阳,王 楠,谢 林.从筋骨理论探讨神经根型颈椎病治疗机制[J].陕西中医,2022,(12):1771.[doi:DOI:10.3969/j.issn.1000-7369.2022.12.029]
　FANG Xiaoyang,WANG Nan,XIE Lin.Treatment mechanism of cervical spondylotic radiculopathy based on theory of Jingu[J].,2022,(1):1771.[doi:DOI:10.3969/j.issn.1000-7369.2022.12.029]
[6]董春力,赵元廷,曹 毅,等.芍药甘草汤联合神经松动术治疗腰椎间盘突出症合并坐骨神经痛疗效研究[J].陕西中医,2023,(9):1245.[doi:DOI:10.3969/j.issn.1000-7369.2023.09.020]
[7]赖剑萍,薛爱国.温针灸结合通瘀煎治疗神经根型颈椎病气滞血瘀证疗效研究[J].陕西中医,2023,(9):1305.[doi:DOI:10.3969/j.issn.1000-7369.2023.09.035]
[8]曾雪琴,董 博,李亚峰,等.冲击波联合肌筋膜触发点治疗神经根型颈椎病疗效观察[J].陕西中医,2024,(1):50.[doi:DOI:10.3969/j.issn.1000-7369.2024.01.011]
　ZENG Xueqin,DONG Bo,LI Yafeng,et al.Effect of extracorporeal shock wave combined with myofascial trigger point in the treatment of cervical spondylotic radiculopathy[J].,2024,(1):50.[doi:DOI:10.3969/j.issn.1000-7369.2024.01.011]
[9]李 吉,杨 锋,白小军,等.名中医李彦民治疗神经根型颈椎病临床经验[J].陕西中医,2024,(6):824.[doi:DOI:10.3969/j.issn.1000-7369.2024.06.022]

备注/Memo

备注/Memo:
基金项目:甘肃省自然科学基金资助项目(21CX1RA176); 甘肃省中医药管理局课题(GZK-2016-53); 西北民族大学中央高校基本科研业务费专项资金资助项目(31920160109)
更新日期/Last Update: 2022-01-09