[1]杨春静,时正媛,续茜桥,等.静宁颗粒对H2O2诱导的原代皮层神经元氧化应激的保护作用研究[J].陕西中医,2022,(2):145-150.[doi:DOI:10.3969/j.issn.1000-7369.2022.02.002]
　YANG Chunjing,SHI Zhengyuan,XU Xiqiao,et al.Protective effect of Jingning granule on H2O2 induced oxidative stress in primary cortical neurons[J].,2022,(2):145-150.[doi:DOI:10.3969/j.issn.1000-7369.2022.02.002]
点击复制

静宁颗粒对H2O2诱导的原代皮层神经元氧化应激的保护作用研究
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年2期
页码:
145-150
栏目:
基础研究
出版日期:
2022-02-05

文章信息/Info

Title:
Protective effect of Jingning granule on H2O2 induced oxidative stress in primary cortical neurons
作者:
杨春静1; 时正媛1; 续茜桥1; 宝 丽1; 游龙泰2; 倪 健2
(1.首都医科大学附属北京世纪坛医院,北京 100038; 2.北京中医药大学中药学院,北京 100029)
Author(s):
YANG Chunjing;  SHI Zhengyuan; XU Xiqiao;  BAO Li; YOU Longtai;  NI Jian
(Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China)
关键词:
注意缺陷多动障碍;  氧化应激;  静宁颗粒;  原代皮层神经元;  神经保护;  过氧化氢;  信号通路
Keywords:
Attention deficit hyperactivity disorder;  Oxidative stress;  Jingning granule;  Primary cortical neurons;  Neuroprotection;  H2O2;  Signal pathway
分类号:
R 725
DOI:
DOI:10.3969/j.issn.1000-7369.2022.02.002
文献标志码:
A
摘要:
目的:探讨静宁颗粒对小鼠原代皮层神经元氧化损伤的保护作用。方法:实验分为正常组、模型组和各用药组。采用过氧化氢(H2O2)刺激造成原代皮层神经元氧化损伤模型,各用药组采用0.2、0.4、0.8 mg/ml的静宁颗粒进行干预。采用MTT法检测活性抑制率,采用试剂盒检测活性氧(ROS)、丙二醛(MDA)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)活力; Western blot检测kelch样ECH关联蛋白1(Keap1)、红系衍生的核转录相关因子2(Nrf2)、促凋亡蛋白(Bax)、B淋巴细胞瘤-2基因(Bcl-2)及含半胱氨酸的天冬氨酸蛋白水解酶3(Caspase 3)等蛋白的表达。结果:静宁颗粒干预后较模型组相比ROS、MDA降低,GSH、SOD升高; 与模型组比较,Keap1、p53、Bax及Caspase 3蛋白表达下调,Nrf2、NQO1、HO-1及Bcl-2蛋白表达升高。结论:静宁颗粒可通过Keap1/Nrf2氧化应激通路和Caspase依赖的线粒体通路对H2O2所致小鼠原代皮层神经元氧化应激损伤发挥保护作用。
Abstract:
Objective:To investigate the protective effect of Jingning granule on oxidative damage of primary cortical neurons extracted from mice.Methods:The experiment was divided into normal group,model group and administration group.The oxidative damage model of primary cortical neurons caused by hydrogen peroxide(H2O2)stimulation was used.The administration group was intervened with Jingning granules of 0.2,0.4,0.8 mg/ml.MTT assay was used to detect the inhibition rate.The kits were used to detect the activity of ROS,MDA,GSH and SOD; the protein expression of Kelch-like ECH associated protein1(Keap1),Nuclear factor(erythroid-derived2)like 2 protein(Nrf2),Bax,B-cell lymphoma-2(Bcl-2)and cysteinyl aspartate specific proteinase(Caspase 3)is detected by Western blot.Results:Compared with the model group,Jingning granule decreased ROS,MDA,and increased GSH,SOD.The expression of Keap1,p53,Bax and Caspase 3 protein was down-regulated,and Nrf2,NQO1,HO-1 and Bcl-2 was up-regulated compared with the model group.Conclusion:Jingning granule can protect primary cortical neurons from oxidative stress damage induced by H2O2 through Keap1/Nrf2 oxidative stress pathway and Caspase dependent mitochondrial pathway.

参考文献/References:

[1] Saedisomeolia A,Samadi M,Gholami F,et al.Vitamin D's molecular action mechanism in attention-deficit/ hyperactivity disorder:A review of evidence[J].CNS Neurol Disord Drug Targets,2018,17(4):280-290.
[2] Corona JC.Role of oxidative stress and neuroinflammation in attention-deficit/hyperactivity disorder[J].Antioxidants,2020,9(11):1039.
[3] Chang CH,Yu CJ,Du JC,et al.The associations among organophosphate pesticide exposure,oxidative stress,and genetic polymorphisms of paraoxonases in children with attention deficit/hyperactivity disorder[J].Science of The Total Environment,2021,773(15):145604.
[4] Lourdes AA,Nadia GG,Marcela SG,et al.Antioxidants as a potential target against inflammation and oxidative stress in attention-deficit/hyperactivity disorder[J].Antioxidants,2020,9(2):176.
[5] Groenman AP,Schweren LJ,Dietrich A,et al.An update on the safety of psychostimulants for the treatment of attention-deficit/hyperactivity disorder[J].Expert Opinion on Drug Safety,2017,16(4):455-464.
[6] 方琼杰,王俊宏,丁一芸,等.静宁颗粒对注意力缺陷多动障碍模型大鼠认知功能及纹状体单胺类的影响[J].世界中西医结合杂志,2020,15(2):273-277.
[7] 庞 敏,吴 志,陈 森,等.重楼皂苷Ⅰ对成骨细胞MC3T3-E1增殖及分化的实验研究[J].陕西医学杂志,2019,48(9):1111-1114.
[8] 朱美霖,白宏英.Purmorphamine对脑缺血再灌注大鼠细胞凋亡影响的实验研究[J].陕西医学杂志,2021,50(1):7-9,21.
[9] 王 颖,周 玥,张立石,等.中医药治疗注意缺陷多动障碍的药理研究进展[J].中药新药与临床药理,2020,31(10):1256-1263.
[10] Pastura G,Kubo TT,Gasparetto EL,et al.Pilot study of brain morphometry in a sample of Brazilian children with attention deficit hyperactivity disorder:Influence of clinical presentation[J].Clinical Neuroradiology,2017,27(4):503-509.
[11] Jung M,Mizuno Y,Fujisawa TX,et al.The Effects of COMT polymorphism on cortical thickness and surface area abnormalities in children with ADHD[J].Cerebral Cortex,2019,29(7):3902-3911.
[12] Sanchez V,Castellanos FX.Neuroimaging in attention-deficit/hyperactivity disorder[J].Current Opinion in Psychiatry,2021,34(2):105-111.
[13] Kibby MY,Dyer SM,Lee SE,et al.Frontal volume as a potential source of the comorbidity between attention-deficit/hyperactivity disorder and reading disorders[J].Behavioural Brain Research,2020,381(2):112382.
[14] Prasad KN.Simultaneous activation of Nrf2 and elevation of antioxidant compounds for reducing oxidative stress and chronic inflammation in human Alzheimer's disease[J].Mechanisms of Ageing & Development,2016,153(23):41-47.
[15] Yang JE,Jia N,Wang D,et al.Ginsenoside Rb1 regulates neuronal injury and Keap1-Nrf2/ARE signaling pathway in cerebral infarction rats[J].Journal of Biological Regulators and Homeostatic Agents,2020,34(3):1091-1095.
[16] 侯书鹏.银杏内酯B通过Nrf2/HO-1通路抑制糖尿病大鼠肝损伤机制研究[J].陕西中医,2021,42(8):1005-1009.
[17] Guo F,Yang F,Zhu YH.Scutellarein from scutellaria barbata induces apoptosis of human colon cancer HCT116 cells through the ROS-mediated mitochondria-dependent pathway[J].Natural Product Research,2019,33(16):2372-2375.
[18] Liu TF,Han YW,Zhou T,et al.Mechanisms of ROS-induced mitochondria-dependent apoptosis underlying liquid storage of goat spermatozoa[J].Aging,2019,11(18):7880-7898.
[19] Xi Y,Wang WW,Wang L,et al.Triptolide induces p53-dependent cardiotoxicity through mitochondrial membrane permeabilization in cardiomyocytes[J].Toxicology and Applied Pharmacology,2018,355(20):269-285.
[20] Li ZZ,Deng WJ,Cao AL,et al.Huangqi decoction inhibits hyperglycemia-induced podocyte apoptosis by down-regulated Nox4/p53/Bax signaling in vitro and in vivo[J].American Journal of Translational Research,2019,11(5):3195-3212.
[21] 杨 繁,曾永蕾.苦参碱对宫颈癌细胞中Bcl-2/Bax表达水平及生物学行为的影响[J].陕西中医,2021,42(7):840-843.

相似文献/References:

[1]玉山江·艾克木,哈丽达·木沙△,地丽尼嘎尔.西红康联合还原型谷胱甘肽治疗2型糖尿病肾病Ⅲ期气阴两虚夹瘀证疗效及对患者微炎症状态、抗氧化的影响*[J].陕西中医,2020,(3):338.
　Yushanjiang·Aikemu,Halida·Musha,Dilinigaer..Efficacy of adjuvant therapy of Xihongkang on type 2 diabetic nephropathy Ⅲ phase with syndrome of Qiyin Liangxu Jiayu type and its effect on micro inflammation and antioxidant role[J].,2020,(2):338.
[2]冯 颖,苏衍进△,司海龙,等.水陆地黄汤联合盐酸贝那普利对气阴两虚夹瘀型糖尿病肾病氧化应激及临床指标的影响*[J].陕西中医,2020,(5):605.[doi:DOI:10.3969/j.issn.10007369.2020.05.014]
　FENG Ying,SU Yanjin,SI Hailong,et al.Effects of Shuilu Dihuang decoction combined with Benazepril hydrochloride on oxidative stress and clinical indicators in type 2 diabetic nephropathy with deficiency of both qi and Yin and blood stasis[J].,2020,(2):605.[doi:DOI:10.3969/j.issn.10007369.2020.05.014]
[3]程小丽,杨世峰△,师锁江,等.黄芪附子方治疗慢性肾功能衰竭维持性血液透析疗效及对患者氧化应激、微炎症反应和生活质量的影响*[J].陕西中医,2020,(10):1436.[doi:DOI:10.3969/j.issn.1000-7369.2020.10.026]
[4]丁 维,钟立仁,张海波,等.丹参酮ⅡA磺酸钠对高脂血症勃起功能障碍大鼠Nrf2/HO-1通路及勃起功能的影响*[J].陕西中医,2020,(11):1520.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.002]
　DING Wei,ZHONG Liren,ZHANG Haibo,et al.Sodium tanshinone ⅡA sulfonate ameliorates erectile function in hyperlipidemia ED rats by up-regulating Nrf2/HO-1[J].,2020,(2):1520.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.002]
[5]欧阳雅蓉,傅为武,黄彩依,等.自噬在高血压靶器官损害中的作用及中医药治疗研究进展*[J].陕西中医,2020,(11):1669.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.040]
[6]黄 钢,马善波,杨双武,等.鹅不食草介导Nrf2/HO-1信号通路在脑缺血再灌注中抗氧化抗炎作用机制研究*[J].陕西中医,2020,(12):1699.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.005]
　HUANG Gang,MA Shanbo,YANG Shuangwu,et al.Centipeda minima mediated anti-oxidation and anti-inflammatory through Nrf2/HO-1 pathway in cerebral ischemia-reperfusion[J].,2020,(2):1699.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.005]
[7]陈秀娟,朱昭明,苏宝印.参芪清毒胶囊对血液透析患者营养指标、氧化应激、炎性因子、残余肾功能的影响[J].陕西中医,2021,(3):334.[doi:DOI:10.3969/j.issn.1000-7369.2020.03.016]
　CHEN Xiujuan,ZHU Zhaoming,SU Baoyin.Influence of Shenqi Qingdu capsule on nutrition,oxidative stress,inflammatory factor and residual renal function in hemodialysis patients[J].,2021,(2):334.[doi:DOI:10.3969/j.issn.1000-7369.2020.03.016]
[8]高加胜,鲁 建,钱雅琴,等.紫正地黄汤联合孟鲁司特钠治疗儿童腺样体肥大疗效及对患儿血清变态反应指标、氧化应激的影响[J].陕西中医,2021,(9):1247.[doi:DOI:10.3969/j.issn.1000-7369.2020.09.025]
　GAO Jiasheng,LU Jian,QIAN Yaqin,et al.Effect of Zizheng Dihuang decoction combined with montelukast sodium on serum allergic reaction and oxidative stress in children with adenoid hypertrophy[J].,2021,(2):1247.[doi:DOI:10.3969/j.issn.1000-7369.2020.09.025]
[9]蔡 菁,葛亚强,许丽娟,等.蒿属香豆素对MCD饮食诱导的非酒精性脂肪性肝纤维化小鼠保护性作用的研究[J].陕西中医,2021,(11):1511.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.004]
　CAI Jing,GE Yaqiang,XU Lijuan,et al.Protective effect of scoparone on MCD diet induced liver fibrosis in mice[J].,2021,(2):1511.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.004]
[10]王朝歆,李 岩,王 超,等.芎归六君子汤治疗急性心肌梗死疗效及对患者再灌注损伤后心功能、氧化应激水平的影响[J].陕西中医,2021,(11):1549.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.012]
　WANG Chaoxin,LI Yan,WANG Chao,et al.Effect of Xionggui Liujunzi decoction on cardiac function and oxidative stress level in patients with acute myocardial infarction after reperfusion injury[J].,2021,(2):1549.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.012]

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81973492); 首都医科大学附属北京世纪坛医院基金资助项目(021-C03)
更新日期/Last Update: 2022-02-09