[1]陈 琼,金毓莉,杭远远,等.育肾助孕方对卵巢储备功能降低大鼠卵巢颗粒细胞ERK1/2、JNK1/2蛋白活化的影响[J].陕西中医,2022,(4):411-415.[doi:DOI:10.3969/j.issn.1000-7369.2022.04.001]
　CHEN Qiong,JIN Yuli,HANG Yuanyuan,et al.Effects of Yushen Zhuyun decoction on activation of ERK1/2 and JNK1/2 proteins in ovarian granulosa cells of rats with diminished ovarian reserve[J].,2022,(4):411-415.[doi:DOI:10.3969/j.issn.1000-7369.2022.04.001]
点击复制

育肾助孕方对卵巢储备功能降低大鼠卵巢颗粒细胞ERK1/2、JNK1/2蛋白活化的影响
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年4期
页码:
411-415
栏目:
基础研究
出版日期:
2022-04-05

文章信息/Info

Title:
Effects of Yushen Zhuyun decoction on activation of ERK1/2 and JNK1/2 proteins in ovarian granulosa cells of rats with diminished ovarian reserve
作者:
陈 琼1; 金毓莉2; 杭远远1; 徐 梅3; 唐苾芯4
(1.上海交通大学附属第一人民医院,上海 200080; 2.上海中医药大学附属岳阳中西医结合医院,上海 200437; 3.上海市黄浦区中西医结合医院,上海 200010; 4.上海市浦东新区公利医院,上海 200135)
Author(s):
CHEN Qiong; JIN Yuli; HANG Yuanyuan; XU Mei; TANG Bixin
(Shanghai General Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200080,China)
关键词:
卵巢储备功能降低;  育肾助孕方;  大鼠;  卵巢颗粒细胞;  细胞凋亡;  细胞外信号调节蛋白激酶1/2
Keywords:
Diminished ovarian reserve;  Yushen Zhuyun decoction;  Rats;  Ovarian granulosa cells;  Cells apoptosis;  Extracellular signal regulated protein kinase 1/2
分类号:
R 711.75
DOI:
DOI:10.3969/j.issn.1000-7369.2022.04.001
文献标志码:
A
摘要:
目的:观察育肾助孕方对卵巢储备功能降低(DOR)大鼠卵巢颗粒细胞卵巢组织细胞外信号调节蛋白激酶1/2(ERK1/2)、c-Jun氨基末端激酶1/2(JNK1/2)蛋白活化的影响,初步探讨其作用机制。方法:选择48只雌性SD大鼠随机分为六组:对照组、模型组、补佳乐组和育肾助孕方低、中、高剂量组,每组8只。除对照组以外,其他各组均采用雷公藤多苷药液灌胃构建卵巢储备功能降低大鼠模型。模型组和对照组给予3 ml 0.9%氯化钠水溶液灌胃; 补佳乐组采用戊酸雌二醇药液灌胃给药; 育肾助孕方低、中、高剂量组大鼠分别采用0.69、1.38、2.76 g/kg育肾助孕方灌胃给药; 各组大鼠均每天定时灌胃给药1次,连续14 d。HE染色观察卵巢组织形态变化; Western blot(WB)法检测ERK1/2及磷酸化细胞外信号调节蛋白激酶1/2(p-ERK1/2)、JNK1/2及磷酸化c-Jun氨基末端激酶1/2(p-JNK1/2)蛋白表达情况。结果:模型组卵巢组织萎缩,卵泡数目及黄体数目减少; 补佳乐组、育肾助孕方中、高剂量组卵巢组织形态接近对照组,卵泡及黄体数目较模型组增加; 与对照组比较,模型组、育肾助孕方低剂量组p-ERK1/2及p-JNK1/2蛋白表达均明显升高(P<0.05),补佳乐组、育肾助孕方高剂量组p-ERK1/2及p-JNK1/2表达比较差异均无统计学意义(P>0.05); 与模型组比较,补佳乐组及育肾助孕方各组p-ERK1/2及p-JNK1/2蛋白表达均明显降低(P<0.05); 与补佳乐组比较,育肾助孕方各组p-ERK1/2及育肾助孕方中、高剂量组p-JNK1/2蛋白表达差异无统计学意义(P>0.05); 育肾助孕方低剂量组p-JNK1/2蛋白表达明显高于补佳乐组(P<0.05)。结论:大鼠卵巢储备功能降低与p-ERK1/2、p-JNK1/2蛋白表达增多有关; 育肾助孕方可能通过降低p-ERK1/2、p-JNK1/2蛋白活化,减少卵巢颗粒细胞凋亡,改善DOR大鼠卵巢功能,减缓衰退进程,以中、高剂量组效果较好。
Abstract:
Objective:To investigate the working methodology of Yushen Zhuyun decoction by observing its effects on activation of extracellular signal regulated protein kinase 1/2(ERK1/2)and c-Jun amino terminal kinase 1/2(JNK1/2)proteins in ovarian granulosa cells of rats with diminished ovarian reserve(DOR).Methods:48 female SD rats were randomly divided into 6 groups:the control group,the model group,the Progynova group and three Yushen Zhuyun decoction groups with low,medium and high dose,with 8 rats in each group.Except for the control group,rats of other groups with diminished ovarian reserve function were established by oral administration with tripterygium polyglycosides.Both the model group and the blank group were given 3 ml normal saline by gavage; In the Progynova group,estradiol valerate was administered by gavage; Rats in low,medium and high dose Yushen Zhuyun decoction groups were treated with 0.69,1.38 and 2.76 g/kg Yushen Zhuyun decoction respectively by gavage; Each group was given gavage once a day,lasting 14 days.The morphological changes of ovary were observed by HE staining,while the expression levels of(ERK1/2),phosphorylated extracellular signal regulated protein kinase 1/2(p-ERK1/2),JNK1/2 and phosphorylated c-Jun amino terminal kinase 1/2(p-JNK1/2)were detected by Western blotting approach.Results:In the model group,the ovarian tissue atrophied with the number of follicles and corpus luteum decreased; The ovarian tissue morphology of the Progynova group,the medium-dose and the high-dose Yushen Zhuyun decoction groups were similar to that of the control group,while the numbers of follicles and corpus luteum of these groups were increased compared with that of the model group.Compared with the control group,the expression level of p-ERK1/2 and p-JNK1/2 protein in the model group and the low-dose Yushen Zhuyun decoction group increased significantly(P<0.05),while no difference was observed between those in the progynova group and the high-dose Yushen Zhuyun decoction group(P>0.05).Compared with the model group,the expression levels of p-ERK1/2 and p-JNK1/2 protein in progynova group and in all three Yushen Zhuyun decoction groups decreased significantly(P<0.05).Compared with progynova group,there was no difference in the expression level of p-ERK1/2 protein in all three Yushen Zhuyun decoction groups,and in that of p-JNK1/2 protein in the medium-dose and high-dose Yushen Zhuyun decoction groups(P>0.05).The expression level of p-JNK1/2 protein in low-dose Yushen Zhuyun decoction group was significantly higher than that in progynova group(P<0.05).Conclusion:The diminishing process of ovarian reserve function in rats is related to higher expression level of p-ERK1/2 and p-JNK1/2 proteins.By reducing the activation of p-ERK1/2 and p-JNK1/2 proteins,the Yushen Zhuyun decoction may diminish the apoptosis of ovarian granulosa cells,improve the ovarian function of DOR rats,and retard the decline process.Moreover,better results can be observed in medium-dose and high-dose Yushen Zhuyun decoction groups.

参考文献/References:

[1] Practice Committee of the American Society for Reproductive Medicine.Testing and interpreting measures of ovarian reserve:A committee opinion[J].Fertility and Sterility,2020,114(6):1151-1157.
[2] 陈 颖,扎 西.不同促排卵方案在卵巢功能减退患者中的有效性观察[J].成都医学院学报,2014,9(1):28-30.
[3] 王文琼.免疫性抗体对女性不孕不育检测的临床意义[J].中国实用医药,2016,11(27):156-157.
[4] 杭远远,张婷婷,金毓莉,等.育肾助孕方对卵巢储备功能降低大鼠卵巢Bcl-2、Bax、Caspase-3表达的影响[J].上海中医药杂志,2019,53(11):76-81.
[5] 金毓莉,张婷婷,许华云,等.育肾助孕方对雷公藤多苷致卵巢储备下降大鼠卵巢形态和性激素的影响[J].中国中医药信息杂志,2016,23(6):56-59.
[6] 李兴媚,郑立红,刘 丹,等.抑制ERK1/2通路对卡铂诱导人卵巢癌细胞凋亡的影响[J].陕西医学杂志,2016,45(5):522-524,538.
[7] 乔宗惠,吴丽敏.卵巢储备功能下降病因病机及中医药治疗研究进展[J].辽宁中医药大学学报,2017,19(1):88-91.
[8] Faddy MJ,Gosden RG,Gougeon A,et al.Accelerated disappearance of ovarian follicles in mid-life:Implications for forecasting menopause[J].Hum Reproduction,1992,7(836):268-291.
[9] 张甜甜,李红杰,张文红.近10年中药治疗卵巢储备功能减退用药规律研究[J].中国中医药信息杂志,2021,28(2):35-38.
[10] 杜 清,陈 心,尤昭玲,等.雷公藤多苷诱导卵巢功能低下大鼠模型的建立[J].实用妇科内分泌电子杂志,2020,7(10):12-13.
[11] 刘 杰,张 茜,陈 瑛.雷公藤对雌性大鼠性激素水平、卵巢内细胞凋亡以及骨代谢状况影响的联合观察与分析[J].海南医学院学报,2018,24(14):1303-1306.
[12] 刘宴娟,祁爱蓉,路 遥,等.雷公藤甲素对中国仓鼠卵巢细胞凋亡的影响及黄体酮的干预效果[J].广西医学,2019,41(3):341-365.
[13] Davis RJ.Signal transduction by the JNK group of MAP kinases[J].Cell,2000,103(2):239-252.
[14] 王带林,匡继林.匡继林治疗卵巢储备功能下降所致不孕经验[J].湖南中医杂志,2020,36(6):23-24.
[15] 伍 琴,张韫玉,刘慧萍,等.尤昭玲教授治疗早发性卵巢功能不全经验[J].陕西中医,2021,42(7):941-943.
[16] 张 利.黄素英治疗月经过少临证经验[J].陕西中医,2020,41(9):1298-1300.
[17] 郑 玫,连 方,孙振高.补肾中药对肾虚不孕患者卵巢颗粒细胞基因表达干预的研究[J].辽宁中医杂志,2017,44(1):12-15.
[18] 陈 衎,唐金艳,韩 璐.补肾中药干预氧化应激治疗早发性卵巢功能不全的作用机制研究进展[J].实用中医内科杂志,2021,35(8):104-107.
[19] 倪喆鑫,丁 杰,俞 瑾,等.基于网络药理学分析补肾阳补肾阴中药分类依据以及对卵巢功能影响的异同[J].中华中医药学刊,2021,39(5):77-80.
[20] 尚玉洁,陈 赟,卢 苏.补肾活血中药治疗早发性卵巢功能不全的Meta分析[J].中华中医药学刊,2018,36(10):2448-2454.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金青年科学基金资助项目(82104908); 上海市卫生和计划生育委员会中医药科研课题(2018LQ013); 上海市松江区科学技术攻关项目(18SJKJGG29); 上海市浦东新区“国家中医药发展综合改革试验区”建设项目(PDZY-2019-0704)
更新日期/Last Update: 2022-04-08