[1]杨 涛,李佳思凡,靳圆圆,等.消肿喷剂主药对体外炎症模型小鼠白细胞介素及NLRP3相关蛋白表达影响的研究[J].陕西中医,2022,(5):571-574.[doi:DOI:10.3969/j.issn.1000-7369.2022.05.006]
 YANG Tao,LI Jiasifan,JIN Yuanyuan,et al.Study on the effect of main drug of detumescence spray on the expression of interleukin and NLRP3 related proteins in inflammation model mouse in vitro[J].,2022,(5):571-574.[doi:DOI:10.3969/j.issn.1000-7369.2022.05.006]
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消肿喷剂主药对体外炎症模型小鼠白细胞介素及NLRP3相关蛋白表达影响的研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年5期
页码:
571-574
栏目:
基础研究
出版日期:
2022-05-05

文章信息/Info

Title:
Study on the effect of main drug of detumescence spray on the expression of interleukin and NLRP3 related proteins in inflammation model mouse in vitro
作者:
杨 涛李佳思凡靳圆圆王水泉牛淑亮
(新疆医科大学,新疆 乌鲁木齐830011)
Author(s):
YANG TaoLI JiasifanJIN YuanyuanWANG ShuiquanNIU Shuliang
(Xinjiang Medical University,Urumqi 830011,China)
关键词:
骨骼肌损伤 消肿喷剂 体外炎症模型 一氧化氮 小鼠
Keywords:
Muscle injury Detumescence spray In vitro inflammatory model NO Mouse
分类号:
R 685
DOI:
DOI:10.3969/j.issn.1000-7369.2022.05.006
文献标志码:
A
摘要:
目的:研究消肿喷剂促进急性骨骼肌损伤炎症修复的作用机制,及炎症在急性骨骼肌损伤修复过程中的作用机制。方法:采用脂多糖(LPS)诱导C2C12小鼠成肌细胞,建立体外细胞炎性模型,ELISA试剂盒检测C2C12细胞上清中白细胞介素(IL-1β、IL-18)含量,qRT-PCR检测C2C12细胞ASC、Caspase-1、NLRP3基因表达,Western blot检测ASC、Caspase-1、NLRP3蛋白的表达情况。结果:NO抑制剂(L-NAME)、NO供体(SNP)能够明显提高或降低IL-1β、IL-18,ASC、Caspase-1和NLRP3 mRNA表达水平和蛋白水平,消肿喷剂主药含药血清能够显著降低IL-1β、IL-18及ASC、Caspase-1、NLRP3 mRNA表达水平和蛋白水平。结论:LPS激活NO-NLRP3-IL炎症轴,诱导建立C2C12细胞体外炎症模型。NO通过NO/ASC/Caspase-1/NLRP3/IL-1β/IL-18轴,调控骨骼肌体外炎症模型炎症因子表达,消肿喷剂可能通过干预NO表达,减轻炎性反应,促进骨骼肌损伤修复。
Abstract:
Objective:To study the mechanism of detumescence spray promoting the inflammatory repair of acute skeletal muscle injury and the mechanism of inflammation in the process of acute skeletal muscle injury repair.Methods:LPS induced C2C12 mouse myoblasts were used to create an in vitro inflammatory model.IL-1β and IL-18 levels in superserum of C2C12 cells were detected by ELISA kit.The expression of ASC,Caspase-1 and NLRP3 genes in C2C12 cells was detected by qRT-PCR and the expression of ASC,Caspase-1 and NLRP3 proteins was detected by Western blot.Results:L-NAME and SNP can significantly increase or decrease IL-1β,IL-18,ASC,Caspase-1 and NLRP3 mRNA expression levels and protein levels.The main drug containing serum of detumescence spray can significantly reduce the mRNA expression levels of IL-1β,IL-18,ASC,Caspase-1,NLRP3 and protein levels.Conclusion:LPS-induced inflammatory model of C2C12 cells in vitro activates the NO-NLRP3-IL inflammatory axis,and NO regulates the expression of inflammatory factors in skeletal muscle inflammatory model through the NO/ASC/Caspase-1/NLRP3/IL-1β/IL-18 axis.Detumescence spray may reduce inflammation and promote skeletal muscle injury repair by interfering with NO expression.

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备注/Memo

备注/Memo:
基金项目:新疆维吾尔自治区自然科学基金资助项目(2017D01C215)
更新日期/Last Update: 2022-05-09