[1]闫晓彤,朱 婷,章晓乐,等.补肾活血方联合补佳乐对宫腔粘连大鼠YAP1/TGF-β1信号通路的影响[J].陕西中医,2022,(8):987-991.[doi:DOI:10.3969/j.issn.1000-7369.2022.08.001]
　YAN Xiaotong,ZHU Ting,ZHANG Xiaole,et al.Effect of Bushen Huoxue decoction combined with Progynova on YAP1/TGF-β1 pathway in intrauterine adhesions rats[J].,2022,(8):987-991.[doi:DOI:10.3969/j.issn.1000-7369.2022.08.001]
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补肾活血方联合补佳乐对宫腔粘连大鼠YAP1/TGF-β1信号通路的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年8期
页码:
987-991
栏目:
基础研究
出版日期:
2022-08-05

文章信息/Info

Title:
Effect of Bushen Huoxue decoction combined with Progynova on YAP1/TGF-β1 pathway in intrauterine adhesions rats
作者:
闫晓彤; 朱 婷; 章晓乐; 齐 聪; 蒋国静; 胡攀伟
(上海中医药大学附属曙光医院妇科,上海 201203)
Author(s):
YAN Xiaotong; ZHU Ting; ZHANG Xiaole; QI Cong; JIANG Guojing; HU Panwei
(Department of Gynecology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
关键词:
宫腔粘连;  补肾活血方;  补佳乐;  纤维化;  YAP1/TGF-β1通路;  妊娠结局
Keywords:
Intrauterine adhesions;  Bushen Huoxue decoction;  Progynova;  Fibrosis;  YAP1/TGF-β1 pathway;  Pregnancy outcome
分类号:
R 711.74
DOI:
DOI:10.3969/j.issn.1000-7369.2022.08.001
文献标志码:
A
摘要:
目的:探讨补肾活血方联合补佳乐对宫腔粘连大鼠的疗效及机制。方法:将40只SD雌性大鼠随机分为假手术组、模型组、中药组(补肾活血方)、西药组(补佳乐)、中药+西药组(补肾活血方+补佳乐),每组8只。应用双重感染建立宫腔粘连模型后,灌胃给药4周,HE染色观察子宫内膜病理变化; Masson染色观察子宫内膜纤维化; 免疫组化检测子宫内膜Yes 相关蛋白1(YAP1)及转化生长因子-β1(TGF-β1)蛋白表达。最后将每组剩余大鼠与雄鼠合笼,7 d后记录胚胎孕囊数。结果:与模型组相比,中药+西药组的内膜厚度和腺体数明显改善(P<0.05)。与模型组相比,中药组、西药组、中药+西药组纤维化明显减轻(P<0.05),而与西药组或中药组相比,中药+西药组纤维化程度显著降低(P<0.05)。与模型组相比,中药组、中药+西药组YAP1蛋白表达明显降低(P<0.05); 同西药组或中药组相比,中药+西药组子宫内膜YAP1蛋白表达明显降低(P<0.05)。与模型组相比,中药+西药组TGF-β1蛋白表达明显减少(P<0.05); 同西药组相比,中药+西药组TGF-β1蛋白表达量减少(P<0.05)。与模型组、中药组、西药组相比,中药+西药组子宫孕囊数明显增多(P<0.05)。结论:补肾活血方联合补佳乐能够有效抑制大鼠宫腔粘连,减轻子宫内膜纤维化,改善妊娠结局,其作用机制可能与调控YAP1/TGF-β1信号通路有关。
Abstract:
Objective:To explore the therapeutic effect and mechanism of Bushen Huoxue decoction combined with Progynova in intrauterine adhesions rats.Methods:40 female SD rats were randomly divided into sham group,model group,Chinese medicine group(Bushen Huoxue decoction),western medicine group(Progynova),Chinese+western medicine group(Bushen Huoxue decoction+Progynova),with 8 rats in each group.After the intrauterine adhesion model was established by double infection,rats were administered by gavage for 4 weeks,and the pathological changes of endometrium were observed by HE staining.Masson staining was used to observe endometrial fibrosis.Immunohistochemical was used to detect YAP1 and TGF-β1 protein expression.Finally,the remaining mice in each group were caged with male mice,and the number of gestational sac was recorded after 7 days.Results:Compared with the model group,the intimal thickness and the number of glands in the Chinese + western medicine group were significantly improved(P<0.05).Compared with the model group,the fibrosis of Chinese medicine group,western medicine group and Chinese + western medicine group was significantly reduced(P<0.05),while compared with western medicine group or Chinese medicine group,the degree of fibrosis in Chinese+western medicine group decreased significantly(P<0.05).Compared with the model group,the expression of YAP1 protein of Chinese medicine group and Chinese+western medicine group decreased significantly(P<0.05).Compared with western medicine group or Chinese medicine group,the expression of YAP1 protein in Chinese+western medicine group decreased significantly(P<0.05).Compared with the model group,the expression of TGF-β1 protein in the Chinese+western medicine group decreased significantly(P<0.05).Compared with western medicine group,the expression of TGF-β1 protein in Chinese+western medicine group decreased(P<0.05).Compared with model group,Chinese medicine group or western medicine group,the number of gestational sac in Chinese+western medicine group increased significantly(P<0.05).Conclusion:Bushen Huoxue decoction combined with Progynova can reduce intrauterine adhesions in rats and improve pregnancy outcome by inhibiting YAP1/TGF-β1 pathway.

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备注/Memo

备注/Memo:
基金项目:国家重点研发计划项目(2018YFC1704300); 上海中医药大学附属曙光医院四明青年基金资助项目(SGKJ-201919)
更新日期/Last Update: 2022-08-10