[1]杨 桐,田春洪,田 原,等.益血生髓颗粒对苯试剂诱导的再生障碍性贫血小鼠造血功能及T细胞亚群的影响[J].陕西中医,2022,(8):1019-1023.[doi:DOI:10.3969/j.issn.1000-7369.2022.08.007]
　YANG Tong,TIAN Chunhong,TIAN Yuan,et al.Effects of Yixue Shengsui granule on hematopoietic function and T cell subsets of benzene reagent-induced aplastic anermia model mice[J].,2022,(8):1019-1023.[doi:DOI:10.3969/j.issn.1000-7369.2022.08.007]
点击复制

益血生髓颗粒对苯试剂诱导的再生障碍性贫血小鼠造血功能及T细胞亚群的影响

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年8期
页码:
1019-1023
栏目:
基础研究
出版日期:
2022-08-05

文章信息/Info

Title:
Effects of Yixue Shengsui granule on hematopoietic function and T cell subsets of benzene reagent-induced aplastic anermia model mice
作者:
杨 桐; 田春洪; 田 原; 乔金丽; 杨小洁
(云南省中医中药研究院,云南 昆明 650032)
Author(s):
YANG Tong; TIAN Chunhong; TIAN Yuan; QIAO Jinli; YANG Xiaojie
(Yunnan Institute of Traditional Chinese Medicine,Kunming 650032,China)
关键词:
再生障碍性贫血;  益血生髓颗粒;  造血功能;  T淋巴细胞;  免疫功能
Keywords:
Aplastic anemia;  Yixue Shengsui granule;  Hematopoietic;  T lymphocyte;  Immune function
分类号:
R 556.5
DOI:
DOI:10.3969/j.issn.1000-7369.2022.08.007
文献标志码:
A
摘要:
目的:研究益血生髓颗粒对苯试剂诱导的再生障碍性贫血(AA)模型小鼠造血及免疫功能的影响。方法:72只SPF级BALB/c小鼠随机分为正常对照组、AA模型组、益血生髓颗粒低剂量组(低剂量组)、益血生髓颗粒高剂量组(高剂量组)和司坦唑醇阳性药组(阳性对照组),除正常对照组其他各组小鼠注射苯和玉米油混合液2 ml/kg,每周4次,建立AA模型,正常组注射同体积玉米油,7周后,低剂量组用药1.2 g/(kg·d),高剂量组用药4.8 g/(kg·d),阳性对照药组予司坦唑醇2 mg/(kg·d),连续给药15 d后,检测血细胞(WBC、RBC、HGB、PLT)、骨髓有核细胞数(BMNC)、T淋巴细胞亚群(CD3+CD4+、CD3+CD8+、Th1、Th2)、血清白介素2(IL-2)、粒细胞集落刺激因子(G-CSF)、巨噬细胞集落刺激因子(M-CSF)和HE染色骨髓病理学的变化情况。结果:与AA模型组比较,低、高剂量组和阳性对照组小鼠外周血四种血细胞数量、血清M-CSF、CD3+CD4+T和CD4+/CD8+比值升高(P<0.05),CD3+CD8+T、IL-2水平和Th2细胞百分数降低(P<0.05),骨髓病理学显示骨髓组织增生较模型组活跃,造血细胞增多,非造血细胞减少。结论:益血生髓颗粒可通过对AA小鼠T细胞亚群的免疫调节和促进造血细胞增殖达到一定恢复造血功能的作用。
Abstract:
Objective:To investigate the effect of Yixue Shengsui granule hematopoietic and immune function of benzene reagent-induced aplastic anemia(AA)model mice.Methods:Totally 72 SPF-class BALB/c mice were randomly divided into normal control group,AA model group,Yixue Shengsui granule low-dose,high-dose groups and positive control group.Except for normal control group,other groups were given injected with the corn oil mixture of benzene for 2 ml/kg four times a week to develop AA models.The normal control group mice were injected with corn oil of the same volume.Four weeks later,the AA model mice were treated with Yixue Shengsui granule 1.2 g/(kg·d),4.8 g/(kg·d)by crude drug and stanzozlol 2 mg/(kg·d)by stanzozlol for 15 days.The peripheral hemogram was tested and the number of nucleated cells in bone marrow(BMNC)were calculated; T lymphocyte subset(CD3+CD4+,CD3+CD8+,Th1,Th2)was examined.The interleukin-2(IL-2)levels,granulocyte colony-stimulating factor(G-CSF),macrophage colony-stimulating factor(M-CSF),bone marrow pathology changes of the mice by hematoxylin-eosin staining.Results:Compared with the AA model group,the number of WBC,RBC,HGB,PLT,M-CSF,CD3+CD4+T cells percentage and the CD4+/CD8+ T cells ratio of the mice in low,high doses of Yixue Shengsui granule groups were increased(P<0.05),the CD3+CD8+T cells percentage,Th2 cells percentage,IL-2 levels were decreased(P<0.05),hematopoietic cells in bone marrow tissue were shown increased and non-hematopoietic cells decreased by pathological observation.Conclusion:Yixue Shengsui granule restores hematopoietic function by immunomodulating the subset of T cells and promoting hematopoietic cell proliferation in AA mice.

参考文献/References:

[1] 汤美雯.再生障碍性贫血机制的研究进展[J].医学前沿,2012,2(17):101-102.
[2] Zheng ZY,Yu XL,Dai TY,et al.Panaxdiol saponins component promotes hematopoiesis and modulates T lymphocyte dysregulation in aplastic anemia model mice[J].Chin J Integr Med,2019,25(12):902-910.
[3] 曹宇峰,张琳琳,吕丽丽,等.健脾补肾方对再生障碍性贫血患者免疫T细胞亚群及相关细胞因子的影响[J].河北医药,2020,42(4):508-511,516.
[4] 贺 欣,王化泉,邵宗鸿.Tim3和BAT3在再生障碍性贫血中免疫机制的研究进展[J].中国免疫学杂志,2020,36(5):630-633.
[5] Zeng W,Miyazawa A,Chen G,et al.Interferon gamma-induced gene expression in CD34 cells:Identification of pathologic cytokine-specific signature profiles[J].Blood,2006,107(1):167-175.
[6] 张 震.辨证论治新理念与临床应用[M].上海:上海科学技术出版社,2014:86-89.
[7] 邢海燕,胡春萍,蔡雪婷,等.再生障碍性贫血小鼠造模方法[J].中国老年医学杂志,2013,15(23):5888-5890.
[8] 黄继汉,黄晓晖,陈志扬,等.药理试验中动物间和动物与人体间的等效剂量换算[J].中国临床药理学与治疗学,2004,9(9):1069-1072.
[9] 姚 军,李树浓.淋巴细胞与再生障碍性贫血关系的实验研究[J].中华血液学杂志,1991,12(5):229-231.
[10] 张宇辰.针对不同发病机制建立再生障碍性贫血动物模型的研究概况[J].中国实验血液学杂志,2015,23(1):285-289.
[11] 李 锐,陈文娜,范梦天.苯诱发再生障碍性贫血小鼠模型建立[J].中国公共卫生,2010,26(6):738-739.
[12] 赵 琳,肖 燕,金润铭.再生障碍性贫血发病机制研究进展[J].中国实用儿科杂志,2011,26(7):545-548.
[13] Faiola B,Bauer AK,Fuller ES,et al.Variations in Prkdc and Sus-ceptibility to benzene-induced toxi city in mice[J].Toxicol Sci,2003,75(2):321-332.
[14] Zhang HX,Wu GS,Guo WL.Changes and significances of Th1/Th2,related cytokines and T cell subgroup in aplastic anemia patients[J].J Hainan Med University,2016,22(10):778-782.
[15] 谌海燕,郭 明,刘军霞,等.艾灸膈俞穴对重型再生障碍性贫血模型小鼠免疫调控的影响[J].中华中医药学刊,2019,37(7):1789-1792.
[16] Krause DS,Fackler JM,Civic SI,et al.CD34:Structure,biology and clinical utility[J].Blood,1996,87(1):1-13.
[17] 于天祥.基于IFN-γ/T-bet、IL-4/STAT6信号通路探讨再生障碍性贫血发病机制及补肾健脾祛障方的干预作用[D].济南:山东中医药大学,2017.
[18] 黄莉荣,薛 征.基于Th1/Th2失衡探讨再生障碍性贫血中西医治疗研究进展[J].陕西中医,2021,42(11):1644-1647.
[19] Qiao XH,Xie XT,Shi W,et al.OCH-mediated shift of Th1 and Th2 cytokines by NKT cells in mice with aplastic anemia[J].Medical Oncology(Northwood,London,England),2015,32(3):67.
[20] Mingueneau M,Roncagalli R,Gregoire C,et al.Loss of the LAT adaptor converts antigen-responsive T cells into pathogenic effectors that function independently of the T cell receptor[J].Immunity,2009,31(2):197-208.
[21] 李 芮,刘 健,靳 楠,等.基于网络药理学探讨当归补血汤治疗再生障碍性贫血作用机制[J].陕西中医,2022,43(3):384-389.

相似文献/References:

[1]唐洪屈,刘 娜△,刘 勇,等.从脾肾论治熟地黄在慢性再生障碍性贫血治疗中的应用*[J].陕西中医,2020,(6):780.[doi:DOI:10.3969/j.issn.10007369.2020.06.022]
[2]黄莉荣,薛 征.基于Th1/Th2失衡探讨再生障碍性贫血中西医治疗研究进展[J].陕西中医,2021,(11):1644.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.034]
　HUANG Lirong,XUE Zheng.Discussion on treatment progress of traditional Chinese and western medicine of aplastic anemia based on Th1/Th2[J].,2021,(8):1644.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.034]
[3]李 芮,刘 健,靳 楠,等.基于网络药理学探讨当归补血汤治疗再生障碍性贫血作用机制[J].陕西中医,2022,(3):384.[doi:DOI:10.3969/j.issn.1000-7369.2022.03.028]
[4]何昊兰,鲁英娟,张小莉,等.健脾生血汤联合促红素治疗再生障碍性贫血疗效及对患者中医症候、炎症因子水平的影响[J].陕西中医,2022,(10):1411.[doi:DOI:10.3969/j.issn.1000-7369.2022.10.021]
　HE Haolan,LU Yingjuan,ZHANG Xiaoli,et al.Effect of Jianpi Shengxue decoction combined with erythropoietin on aplastic anemia and its effect on TCM symptoms and inflammatory factors[J].,2022,(8):1411.[doi:DOI:10.3969/j.issn.1000-7369.2022.10.021]
[5]李玎玎,何丹迪,王金环.基于“虫子学说”探讨中医药对再生障碍性贫血免疫调控的研究进展[J].陕西中医,2025,46(8):1136.[doi:DOI:10.3969/j.issn.1000-7369.2025.08.026]
　LI Dingding,HE Dandi,WANG Jinhuan.Exploring the research progress of traditional Chinese medicine on immune regulation of aplastic anemia from insect theories[J].,2025,46(8):1136.[doi:DOI:10.3969/j.issn.1000-7369.2025.08.026]
[6]袁艺心,李柯馨,叶苗青,等.基于“补肝体强肝用”思想探讨长安张氏肝病流派辨治再生障碍性贫血[J].陕西中医,2026,(1):77.[doi:DOI:10.3969/j.issn.1000-7369.2026.01.013]
　YUAN Yixin,LI Kexin,YE Miaoqing,et al.Discussion on the treatment of aplastic anaemia by Chang’an ZHANG’s liver disease school based on the idea of “nourishing the liver’s substance and strengthening the liver’s function”[J].,2026,(8):77.[doi:DOI:10.3969/j.issn.1000-7369.2026.01.013]

备注/Memo

备注/Memo:
基金项目:云南省科技厅中医药应用基础研究联合专项课题[2019FF002(076)]; 云南省卫生科技计划项目(2018NS0079)
更新日期/Last Update: 2022-08-10