[1]付华君,宋文英,田俊斌,等.紫檀芪通过调控细胞自噬对抗大鼠心肌缺血/再灌注损伤[J].陕西中医,2022,(10):1342-1346.[doi:DOI:10.3969/j.issn.1000-7369.2022.10.005]
　FU Huajun,SONG Wenying,TIAN Junbin,et al.Pterostilbene inhibits myocardial ischemia reperfusion injury by regulating autophagy in rats[J].,2022,(10):1342-1346.[doi:DOI:10.3969/j.issn.1000-7369.2022.10.005]
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紫檀芪通过调控细胞自噬对抗大鼠心肌缺血/再灌注损伤
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年10期
页码:
1342-1346
栏目:
基础研究
出版日期:
2022-10-05

文章信息/Info

Title:
Pterostilbene inhibits myocardial ischemia reperfusion injury by regulating autophagy in rats
作者:
付华君1; 宋文英1; 田俊斌2; 李 扬1
(1.陕西省人民医院麻醉科,陕西 西安 710068; 2.西安交通大学第二附属医院麻醉科,陕西 西安 710004)
Author(s):
FU Huajun; SONG Wenying; TIAN Junbin; LI Yang
(Department of Anesthesiology,Shaanxi Provincial People's Hospital,Xi'an 710068,China)
关键词:
肌缺血/再灌注损伤;  紫檀芪;  大鼠;  梗死面积;  细胞自噬;  氧化应激;  乳酸脱氢酶;  超氧化物歧化酶
Keywords:
Myocardial ischemia-reperfusion injury;  Pterostilbene;  Rat;  Infarct area;  Autophagy;  Oxidative stress;  Lactate dehydrogenase;  Superoxide dismutase
分类号:
R 542.4
DOI:
DOI:10.3969/j.issn.1000-7369.2022.10.005
文献标志码:
A
摘要:
目的:观察紫檀芪对大鼠心肌缺血/再灌注损伤(MI/RI)的影响,探讨其保护机制是否与调控心肌自噬有关。方法:72只SD大鼠随机分为六组(n=12),分别为假手术组、MI/RI组、阿司匹林组、紫檀芪低剂量组、紫檀芪中剂量组和紫檀芪高剂量组,干预14 d后,构建MI/RI模型,观察心肌组织病理改变、测定心肌梗死面积及心肌组织中细胞自噬信号相关蛋白Atg5、Beclin1、LC3Ⅰ/Ⅱ和p62蛋白表达水平,测定血清心肌损伤指标乳酸脱氢酶(LDH)和肌酸激酶同工酶(CK-MB),氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)的含量。结果:心肌组织病理学检查提示,与假手术组比较,MI/RI组心肌纤维明显断裂,部分坏死,部分细胞结构模糊; 与MI/RI组比较,各治疗组心肌纤维断裂明显减少,细胞结构较清晰(P<0.05)。与假手术组比较,MI/RI组心肌梗死面积、LDH、CK-MB及MDA显著升高(P<0.05),SOD、CAT及GSH-Px显著降低(P<0.05),Atg5、Beclin1及LC3 Ⅰ/Ⅱ蛋白明显升高(P<0.05),p62蛋白显著降低(P<0.05); 与模型组比较,各治疗组心肌梗死面积、LDH、CK-MB和MDA显著降低(P<0.05),SOD、CAT、GSH-Px明显升高(P<0.05),Atg5、Beclin1及LC3 Ⅰ/Ⅱ 蛋白表达降低(P<0.05),p62蛋白表达升高(P<0.05)。结论:紫檀芪预处理能减轻大鼠MI/RI,其机制可能与调控心肌细胞自噬、提高抗氧化能力,从而减轻心肌细胞损伤有关。
Abstract:
Objective:To observe the effects of Pterostilbene on myocardial ischemia/reperfusion injury(MI/RI)in rats,and explore whether its protective mechanism is related to the regulation of myocardial autophagy.Methods:72 SD rats were randomly divided into 6 groups(n=12),including sham operation group,model group,aspirin group,Pterostilbene low dose group,Pterostilbene medium dose group and Pterostilbene high dose group.MI/RI model was established 14 days after administration. The pathological changes of myocardial tissue were observed,the myocardial infarction size was determined,and the expression levels of autophagy signal-related proteins Atg5,Beclin1,LC3Ⅰ/Ⅱ and p62 in myocardial tissue were determined. The contents of lactate dehydrogenase(LDH),creatine kinase isoenzyme(CK-MB),malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)in blood were determined.Results:Myocardial histopathological examination indicated that myocardial fiber fracture and inflammatory response occurred in MI/RI group compared with sham group.Compared with model group,all treatment groups showed improvement.Compared with sham group,model group had increased myocardial infarction area,LDH and CK-MB,increased MDA,decreased SOD,CAT and GSH-Px,increased Atg5,Beclin1 and LC3Ⅰ/Ⅱ protein,and decreased p62(P<0.05).Compared with model group,myocardial infarction size,LDH and CK-MB decreased,MDA decreased,SOD,CAT and GSH-Px increased,protein expressions of Atg5,Beclin1 and LC3Ⅰ/Ⅱ decreased,and p62 increased in all treatment groups(P<0.05).Conclusion:The pre-treatment of Pterostilbene can reduce MI/RI in rats,and the possible mechanism may be related to the regulation of autophagy of cardiomyocytes and the improvement of antioxidant capacity,thus alleviating the injury of cardiomyocytes.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基础研究计划项目(2018JM7110)
更新日期/Last Update: 2022-10-09