[1]兰雅文,唐 明,王佳宁,等.黄连解毒汤对脑梗死合并胃损伤大鼠干细胞因子/酪氨酸激酶受体信号通路的影响[J].陕西中医,2022,(11):1506-1510.[doi:DOI:10.3969/j.issn.1000-7369.2022.11.004]
　LAN Yawen,TANG Ming,WANG Jianing,et al.Effect of Huanglian Jiedu decoction on SCF/C-kit signaling pathway in rats with cerebral infarction complicated with gastric injury[J].,2022,(11):1506-1510.[doi:DOI:10.3969/j.issn.1000-7369.2022.11.004]
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黄连解毒汤对脑梗死合并胃损伤大鼠干细胞因子/酪氨酸激酶受体信号通路的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年11期
页码:
1506-1510
栏目:
基础研究
出版日期:
2022-11-05

文章信息/Info

Title:
Effect of Huanglian Jiedu decoction on SCF/C-kit signaling pathway in rats with cerebral infarction complicated with gastric injury
作者:
兰雅文1; 唐 明2; 王佳宁2; 修小惠2; 赵素君3; 翟容乾4; 安朋朋2
(1.山东中医药大学,山东 济南 250014; 2.青岛市中医医院,山东 青岛 266033; 3.莱阳市中医医院,山东 莱阳 265200; 4.青岛市第六人民医院,山东 青岛 266700)
Author(s):
LAN Yawen; TANG Ming; WANG Jianing; XIU Xiaohui; ZHAO Sujun; ZHAI Ronggan; AN Pengpeng
(Shandong University of Traditional Chinese Medicine,Jinan 250014,China)
关键词:
脑梗死;  大鼠;  胃损伤;  黄连解毒汤;  奥美拉唑;  干细胞因子/酪氨酸激酶受体信号通路
Keywords:
Cerebral infarction;  Rats;  Gastric injury;  Huanglian Jiedu decoction;  Omeprazole;  SCF/C-kit signaling pathway
分类号:
R 743.3
DOI:
DOI:10.3969/j.issn.1000-7369.2022.11.004
文献标志码:
A
摘要:
目的:探讨黄连解毒汤对脑梗死合并胃损伤大鼠干细胞因子/酪氨酸激酶受体(SCF/C-kit)信号通路的影响。方法:将80只健康10周龄雄性Wistar大鼠随机分为模型组、黄连解毒汤组、奥美拉唑组、假手术组、正常组,以上各组再分设4 d组、7 d组,共10组。采用线栓法建立大脑中动脉梗死模型。黄连解毒汤组给予黄连解毒汤(27 mg/ml)混悬液,奥美拉唑组给予奥美拉唑(15 mg/ml)悬浊液,其余组给予1 ml 0.9%氯化钠溶液灌胃,4 d组连续灌胃4 d,7 d组连续灌胃7 d。采用HE染色法观察大鼠胃组织病理形态学变化,并进行病理损伤评分; 采用实时荧光定量检测大鼠胃组织SCF、C-kit mRNA表达水平; 采用Western blot检测大鼠胃组织中SCF、C-kit蛋白表达水平。结果:与正常组、假手术组比较,模型组、黄连解毒汤组、奥美拉唑组大鼠胃黏膜病理评分均明显升高(4、7 d组均P<0.01),胃组织SCF、C-kit mRNA和蛋白表达水平均明显降低(P<0.01); 正常组、假手术组间比较差异无统计学意义(P>0.05)。与模型组比较,黄连解毒汤组、奥美拉唑组大鼠胃黏膜病理评分均显著降低(4、7 d组均P<0.01),胃组织SCF、C-kit mRNA和蛋白表达水平均明显升高(P<0.01)。与奥美拉唑组比较,黄连解毒汤组大鼠胃黏膜病理评分均明显降低(P<0.05),胃组织SCF、C-kit mRNA和蛋白表达水平均明显升高(P<0.05)。结论:黄连解毒汤对脑梗死合并胃损伤大鼠的胃黏膜具有一定的保护作用,其机制可能与黄连解毒汤升高大鼠胃组织SCF、C-kit mRNA及蛋白的表达水平有关。
Abstract:
Objective:To investigate the effect of Huanglian Jiedu decoction on SCF/C-kit signaling pathway in rats with cerebral infarction complicated with gastric injury.Methods:80 healthy Wistar 10-week-old male rats were randomly divided into model group,Huanglian Jiedu decoction group,omeprazole group,sham operation group,normal group,the above groups were divided into 4 d group,7 d group,a total of 10 groups.Middle cerebral artery infarction model was established by suture method.Huanglian Jiedu decoction group was given Huanglian Jiedu decoction(27 mg/ml)suspension,omeprazole group was given omeprazole(15 mg/ml)suspension,and its group was given 1 ml normal saline by gavage,4 d group was given continuous gavage for 4 days,7 d group was given continuous gavage for 7 days.HE staining was used to observe the pathological morphological changes of gastric tissue in rats,and the pathological damage score was performed.The expression levels of SCF and C-kit mRNA in rat gastric tissues were detected by real-time fluorescence quantitative analysis.The expressions of SCF and C-kit protein in gastric tissue were detected by Western blot.Results:Compared with the normal group and the sham operation group,the pathological scores of gastric mucosa in the model group,Huanglian Jiedu decoction group and omeprazole group were significantly increased(P<0.01 in the 4 th and 7 th day groups).Compared with the model group,the pathological scores of gastric mucosa in Huanglian Jiedu decoction group and omeprazole group were significantly decreased(P<0.01),and the mRNA and protein expressions of SCF and C-kit in gastric tissue were significantly increased(P<0.01).Compared with omeprazole group,the pathological scores of gastric mucosa in Huanglian Jiedu decoction group were significantly decreased(P<0.05),and the mRNA and protein expression levels of SCF and C-kit in gastric tissue were significantly increased(P<0.05).Conclusion:Huanglian Jiedu decoction has a certain protective effect on gastric mucosa of rats with cerebral infarction complicated with gastric injury.The mechanism may be related to the fact that Huanglian Jiedu decoction can increase the expression levels of SCF,C-kit mRNA and protein in gastric tissues of rats.

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备注/Memo

备注/Memo:
基金项目:山东省中医药科技发展计划项目(2017-321); 山东省青岛市民生科技计划项目(18-6-1-69-NSH)
更新日期/Last Update: 2022-11-09