[1]孙之中,刘朝红,陈其华,等.象皮生肌膏对糖尿病足溃疡大鼠创面组织内上皮-间质转化过程的影响[J].陕西中医,2022,(12):1664-1668.[doi:DOI:10.3969/j.issn.1000-7369.2022.12.002]
　SUN Zhizhong,LIU Zhaohong,CHEN Qihua,et al.Effects of Xiangpi Shengji ointment on EMT in wound tissues of diabetic ulcer rats[J].,2022,(12):1664-1668.[doi:DOI:10.3969/j.issn.1000-7369.2022.12.002]
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象皮生肌膏对糖尿病足溃疡大鼠创面组织内上皮-间质转化过程的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年12期
页码:
1664-1668
栏目:
基础研究
出版日期:
2022-12-20

文章信息/Info

Title:
Effects of Xiangpi Shengji ointment on EMT in wound tissues of diabetic ulcer rats
作者:
孙之中1; 刘朝红1; 陈其华1; 易 倩1; 羊 羡1; 赵 姣2; 林雅思2; 何 欢2; 刘均瑜2
(1.湖南中医药大学第一附属医院,湖南 长沙410007; 2.湖南中医药大学,湖南 长沙410007)
Author(s):
SUN Zhizhong; LIU Zhaohong; CHEN Qihua; YI Qian; YANG Xian; ZHAO Jiao; LIN Yasi; HE Huan; LIU Junyu
(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)
关键词:
糖尿病足溃疡;  象皮生肌膏;  转化生长因子-β1;  胰岛素样生长因子-1;  纤维结合蛋白;  上皮-间质转化
Keywords:
Diabetic foot ulcer;  Xiangpi Shengji ointment;  Transforming growth factor β1;  Insulin-like growth factor-1;  Fibronectin;  Epithelial-mesenchymal transition
分类号:
R 587.1
DOI:
DOI:10.3969/j.issn.1000-7369.2022.12.002
文献标志码:
A
摘要:
目的:观察象皮生肌膏对糖尿病足溃疡大鼠创面组织内转化生长因子-β1(TGF-β1)、胰岛素样生长因子-1(IGF-1)、纤维结合蛋白(FN)表达的影响,以及在愈合过程中上皮-间质转化(EMT)过程。方法:选择SPF级雄性SD大鼠40只,建立溃疡大鼠模型,其中10只作为空白组,其余30只大鼠建立糖尿病大鼠模型,造模成功后分为模型组、对照组、实验组。比较各组大鼠第7、14天创面愈合情况; 实时荧光定量聚合酶链式反应法(RT-qPCR)检测各组第7、14天TGF-β1、IGF-1、FN的mRNA表达情况; Western blotting检测各组第7、14天EMT相关蛋白E-钙黏蛋白(E-cadherin)、纤连蛋白( Fibronectin)蛋白表达情况; 酶联免疫反应法测定组织中血管内皮生长因子(VEGF)含量。结果:干预第7天,空白组、实验组的创面愈合率均高于模型组; 与模型组相比,空白组、实验组E-cadherin表达降低,Fibronectin表达升高,而TGF-β1、IGF-1、FN的mRNA表达均高; 与对照组相比,实验组E-cadherin蛋白表达降低,Fibronectin蛋白表达升高; 与模型组相比,空白组、对照组、实验组的VEGF含量均高,差异有统计学意义(均P<0.05)。干预第14天,模型组的创面愈合率低于其他组; 与模型组相比,空白组、实验组E-cadherin蛋白表达较低,Fibronectin蛋白表达较高,空白组、对照组、实验组FN mRNA表达均高,空白组、实验组TGF-β1的mRNA表达均高。与模型组相比,空白组、对照组、实验组的VEGF含量均高,差异有统计学意义(均P<0.05)。干预第14天,模型组、对照组、实验组的IGF-1的mRNA表达比较,差异无统计学意义(均P>0.05)。结论:象皮生肌膏能促进EMT过程,加快愈合速度,其途径可能是通过促进TGF-β1表达,介导IGF-1,调控FN实现。
Abstract:
Objective:To observe expression of TGF-β1,IGF-1,FN in wound tissue of diabetic ulcer rats with Xiangpi Shengji ointment,and to explore effect of healing process on epithelial-mesenchymal transition(EMT).Methods:40 male SD rats of SPF grade and establish ulcer rat models.Ten of rats were used as the blank group,and the other 30 rats used to establish diabetic rat models.The 30 rats were successfully divided into model group,control group and experimental group.Compared the wound healing of rats in each group on the 7th and 14th day.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the mRNA expressions of TGF-β1,IGF-1 and FN on the 7th and 14th day in each group.Western blotting was used to detect the protein expression of EMT-related proteins,E-cadherin and Fibronectin on the 7th and 14th day in each group.ELISA was used to measure the content of vascular endothelial growth factor(VEGF)in tissues.Results:On the 7th day of intervention,the wound healing rate of the blank group and the experimental group were significantly higher than those in the model group.On the 7th day of intervention,compared with the model group the expression of E-cadherin in the blank group and the experimental group decreased,expression of Fibronectin increased,and the mRNA expressions of TGF-β1,IGF-1 and FN increased.On the 7th day of intervention,compared with the control group the expression of E-cadherin protein decreased and the expression of Fibronectin protein increased in the experimental group.On the 7th day of intervention,compared with the model group VEGF content of the blank group,the control group and the experimental group were all higher,difference statistically significant(all P<0.05).On the 14th day of intervention,the wound healing rate of the model group was lower than that of the other three groups.On the 14th day of intervention,compared with model group expression of E-cadherin protein in the blank group and the experimental group decreased,but expression of Fibronectin protein increased,expression of FN mRNA in the other three groups increased,expression of TGF-β1 mRNA in the blank group and the experimental group increased.On the 14th day of intervention,compared with the model group the content of VEGF in the blank group,the control group and the experimental group higher,difference statistically significant(all P<0.05).There was no significant difference in IGF-1 mRNA expression among the other three groups except the blank group(all P > 0.05).Conclusion:Xiangpi Shengji ointment can promote the EMT process and accelerate the healing speed.It may be achieved by promoting the expression of TGF-β1 and mediating the regulation of IGF-1 on FN.

参考文献/References:

[1] Eardon R,Simring D,Kim B,et al.The diabetic foot ulcer[J].Aust J Gen Pract,2020,49(5):250-255.
[2] 冯海霞,彭新宇,董 峰.阳和汤加减联合银离子敷料治疗糖尿病足溃疡临床研究[J].陕西中医,2021,42(7):900-902.
[3] 龚海峰,李 强,郝 治,等.以西红花为主药配伍的油剂治疗严重糖尿病足溃疡临床研究[J].陕西中医,2018,39(7):916-918.
[4] 陈凯迪,严 建,曹 晖,等.基于“卫气营血”理论探讨象皮生肌膏在肛瘘术后创面中的应用[J].中国中医基础医学杂志,2021,27(12):1978-1980,1994.
[5] 张新燕,韦 东,胡 明,等.象皮生肌膏对肛瘘术后模型大鼠炎症因子、p38/MK2信号通路和创面组织VEGF、bFGF表达的影响[J].现代生物医学进展,2021,21(17):3242-3246.
[6] Dongre A,Weinberg RA.New insights into the mechanisms of epithelial-mesenchymal transition and implications for cancer[J].Nat Rev Mol Cell Biol,2019,20(2):69-84.
[7] 胡钟元,张秀华,李帅广,等.透明质酸寡糖通过影响核因子E2相关因子2信号通路促进糖尿病皮肤溃疡创面愈合的机制研究[J].中国全科医学,2022,25(26):3281-3289.
[8] 白现花,尹兰英,杨海庆,等.中药熏蒸联合西医常规治疗对糖尿病足溃疡愈合及创面渗出液中VEGF、MMP-2及TIMP-1的影响[J].中医杂志,2018,59(20):1763-1765,1782.
[9] 姜 雄,李木清,毛 滔,等.象皮生肌膏对慢性创面愈合及PTEN、p-AKT、VEGF蛋白表达的影响[J].湖南中医药大学学报,2021,41(1):67-71.
[10] 兰 天,周忠志.象皮生肌膏促糖尿病大鼠皮肤溃疡愈合机制研究[J].中医药导报,2021,27(2):12-15.
[11] 雷 宇,周忠志,兰宏伟,等.象皮生肌膏对糖尿病皮肤溃疡修复的效果及其机理探究[J].中国美容医学,2022,31(1):89-92.
[12] 杜攀攀,李木清,毛 滔,等.象皮生肌膏对慢性创面模型大鼠血清β-EP、PGE-2和5-HT表达的影响[J].中医药导报,2020,26(16):31-34,44.
[13] Manfioletti G,Fedele M.Epithelial-mesenchymal transition(Emt)2021[J].Int J Mol Sci,2022,23(10):5848.
[14] Smina TP,Rabeka M,Viswanathan V.Diabetic foot ulcer as a cause of significant decline in the renal function among south indian population with type 2 diabetes:Role of TGF-β1 and ccn family proteins[J].Int J Low Extrem Wounds,2019,18(4):354-361.
[15] Yao Y,Chen R,Wang G,et al.Exosomes derived from mesenchymal stem cells reverse emt via TGF-β1/smad pathway and promote repair of damaged endometrium[J].Stem Cell Res Ther,2019,10(1):225.
[16] 吕 欣,曹 宇,周达岸.体外冲击波通过调控IGF-1和p-AKT水平促进大鼠骨骼肌损伤的修复[J].中华物理医学与康复杂志,2022,44(4):300-305.
[17] 李博文,冯丽丽,田振军.胰岛素样生长因子-1与代谢性疾病及运动干预的研究进展[J].生理学报,2021,73(2):342-352.
[18] 程学荣,李木清,张月娟,等.象皮生肌膏对慢性创面大鼠肉芽组织TGF-β1、EGFR、FN蛋白表达的影响[J].江西中医药大学学报,2022,34(2):88-92.
[19] 李艳华,唐智军,夏 锴,等.复方鸡子黄油膏对大鼠创面修复中EGFR、FN、TGF-β1表达的影响[J].湖南中医药大学学报,2019,39(12):1449-1452.
[20] 张月娟,李木清,刘 梨,等.象皮生肌膏对2型糖尿病大鼠溃疡肉芽组织转化生长因子β及纤维结合蛋白表达影响的实验研究[J].护理研究,2015,29(31):3872-3875.
[21] Tang G,Li S,Zhang C,et al.Clinical efficacies underlying mechanisms and molecular targets of chinese medicines for diabetic nephropathy treatment and management[J].Acta Pharm Sin B,2021,11(9):2749-2767.
[22] 罗丽娅,蔡玉兰,魏在荣,等.维生素D对糖尿病足溃疡患者组织中维生素D受体、过氧化物酶体增殖物激活受体γ、血管内皮生长因子表达的影响[J].中国糖尿病杂志,2021,29(6):426-432.
[23] 范丽娜,陈丽娟,刘 芳.黄芪多糖对大鼠慢性难愈合创面的作用及其对PTEN、AKT和VEGF蛋白表达的影响[J].中国中医药科技,2022,29(1):36-40.
[24] 左 楠,崔 莉,王 爽.脂肪间充质干细胞对小鼠创面组织超氧化物歧化酶及血管内皮生长因子、表皮生长因子、碱性成纤维细胞生长因子表达的影响[J].陕西医学杂志,2022,51(1):7-10,24.

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备注/Memo

备注/Memo:
基金项目:湖南省临床医疗技术创新引导项目(2020SK51407)
更新日期/Last Update: 2022-12-09