[1]李悦宁,肖柯心,赵冰洁,等.桑玉膏对对乙酰氨基酚诱导的小鼠急性肝损伤保护作用研究[J].陕西中医,2023,(2):154-158.[doi:DOI:10.3969/j.issn.1000-7369.2023.02.004]
　LI Yuening,XIAO Kexin,ZHAO Bingjie,et al.Protective effects of Sangyu ointment against acetaminophen-induced acute liver failure in mice[J].,2023,(2):154-158.[doi:DOI:10.3969/j.issn.1000-7369.2023.02.004]
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桑玉膏对对乙酰氨基酚诱导的小鼠急性肝损伤保护作用研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年2期
页码:
154-158
栏目:
基础研究
出版日期:
2023-02-05

文章信息/Info

Title:
Protective effects of Sangyu ointment against acetaminophen-induced acute liver failure in mice
作者:
李悦宁1; 2; 肖柯心2; 3; 赵冰洁2; 3; 李红玉2; 3; 王 番2; 詹 博2; 3; 贾艳艳1; 2
(1.西北大学生命科学学院,陕西 西安 710069; 2.空军军医大学第一附属医院药剂科,陕西 西安 710032; 3.陕西中医药大学药学院,陕西 咸阳 712046)
Author(s):
LI Yuening; XIAO Kexin; ZHAO Bingjie; LI Hongyu; WANG Fan; ZHAN Bo; JIA Yanyan
(College of Life Sciences,Northwestern University,Xi'an 710069,China)
关键词:
药物性肝损伤;  桑玉膏;  对乙酰氨基酚;  炎症因子;  氧化应激;  保护作用
Keywords:
Drug-induced liver injury;  Sangyu ointment;  Acetaminophen;  Inflammatory factors;  Oxidative stress;  Protective effects
分类号:
R 965.1
DOI:
DOI:10.3969/j.issn.1000-7369.2023.02.004
文献标志码:
A
摘要:
目的:探究桑玉膏对对乙酰氨基酚(APAP)诱导的小鼠肝损伤的保护作用及其作用机制。方法:将60只雄性健康昆明种小鼠随机分成空白对照组,APAP模型组,N-乙酰半胱氨酸(NAC)阳性药物组,桑玉膏低、中、高剂量组,除空白对照组外其他各组腹腔注射APAP,建立药物性肝损伤(DILI)小鼠模型,灌胃给药5 d后,腹腔主动脉取血,收集肝脏组织。生化法检测血清谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(ALP)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、一氧化氮(NO)活性水平或含量,酶联免疫吸附测定法(ELISA)检测肝组织中肿瘤坏死因子-α(TNF-α)、重组人白细胞介素-1β(IL-1β)含量,实时定量PCR法(qRT-PCR)检测肝脏组织中促凋亡蛋白Bax和抑凋亡蛋白Bcl-2的mRNA表达。结果:与APAP模型组比较,桑玉膏各剂量组小鼠ALT、AST、ALP 水平显著降低(P<0.01),肝组织损伤、炎性细胞浸润明显减轻; SOD、GSH-PX活性升高(P<0.01),TNF-α、IL-1β、MDA及NO活性或含量下降(P<0.01),肝脏组织中促凋亡蛋白Bax的mRNA表达显著下降,抑凋亡蛋白Bcl-2的mRNA表达显著升高(P<0.01)。结论:桑玉膏对APAP诱导的小鼠药物性肝损伤有保护作用,其作用机制可能与提高机体抗氧化能力、缓解氧化应激、降低炎症因子水平等有关。
Abstract:
Objective:To investigate the protective effects and mechanism of Sangyu ointment on acute liver failure induced by acetaminophen(APAP)in mice.Methods:Sixty healthy male kunming mice,were randomly divided into blank control group,APAP model group,N-acetylcysteine(NAC)positive drug group and low-dose,middle-dose and high-dose of Sangyu ointment groups.The mice model of drug-induced liver injury was established by intraperitoneal injection of APAP in all groups except blank control group.The blood was collected from abdominal aorta and liver tissue was collected after the mice were given therapeutic intragastric administration for 5 days.The activities of AST,ALT,ALP,MDA,SOD,GSH-PX and NO in serum were detected by biochemical analysis,the contents of TNF-α and IL-1β in liver tissue were detected by ELISA and the mRNA expression of pro-apoptotic protein Bax and anti-apoptotic protein Bcl-2 in liver tissue was detected by qRT-PCR.Results:Compared with APAP model group,the activities of ALT,AST and ALP in the three groups of Sangyu ointment were significantly decreased(P<0.01),liver tissue injury and inflammatory cell infiltration were reduced,and the activities of SOD and GSH-PX were increased(P<0.01).Meanwhile,the contents of TNF-α,IL-1β,MDA and NO decreased in the Sangyu ointment groups(P<0.01).Finally,compared with APAP model group,the mRNA expression of pro-apoptotic protein Bax in liver tissue was decreased,and the mRNA expression of anti-apoptotic protein Bcl-2 was significantly increased in the three groups of Sangyu ointment(P<0.01).Conclusion:Sangyu ointment has a protective effect on drug-induced liver injury induced by APAP in mice,and its mechanism may be related to improving antioxidant capacity,alleviating oxidative stress and reducing the level of inflammatory factors.

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备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2020ZDLSF03-09)
更新日期/Last Update: 2023-02-08