[1]贾燚鑫,宋志慧,高春燕,等.麦冬多糖调控胰高血糖素样肽-1对妊娠期糖尿病大鼠肠道菌群及糖代谢的影响[J].陕西中医,2023,(4):427-432.[doi:DOI:10.3969/j.issn.1000-7369.2023.04.004]
　JIA Yixin,SONG Zhihui,GAO Chunyan,et al.Effects of ophiopogon polysaccharides on intestinal flora and glucose metabolism in gestational diabetic rats by regulating GLP-1[J].,2023,(4):427-432.[doi:DOI:10.3969/j.issn.1000-7369.2023.04.004]
点击复制

麦冬多糖调控胰高血糖素样肽-1对妊娠期糖尿病大鼠肠道菌群及糖代谢的影响
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年4期
页码:
427-432
栏目:
基础研究
出版日期:
2023-04-05

文章信息/Info

Title:
Effects of ophiopogon polysaccharides on intestinal flora and glucose metabolism in gestational diabetic rats by regulating GLP-1
作者:
贾燚鑫; 宋志慧; 高春燕; 高 然
(唐山市妇幼保健院,河北 唐山 063000)
Author(s):
JIA Yixin; SONG Zhihui; GAO Chunyan; GAO Ran
(Tangshan Maternal and Child Health Hospital,Tangshan 063000,China)
关键词:
妊娠期糖尿病;  麦冬多糖;  胰高血糖素样肽-1;  血糖;  肠道菌群;  糖代谢
Keywords:
Gestational diabetes mellitus;  Ophiopogon polysaccharide;  Glucagon-like peptide-1;  Blood glucose;  Intestinal flora;  Glucose metabolism
分类号:
R 587.1
DOI:
DOI:10.3969/j.issn.1000-7369.2023.04.004
文献标志码:
A
摘要:
目的:探究麦冬多糖调控胰高血糖素样肽-1(GLP-1)对妊娠期糖尿病大鼠肠道菌群及糖代谢的影响。方法:将80只孕鼠随机分为空白组、模型组、低剂量组、中剂量组、高剂量组、阳性对照组、Exendin-4组和高剂量+Exendin-4组,每组10只。干预2周后比较各组大鼠空腹血糖(FBG)和胰岛素抵抗指数(HOMA-IR)水平; ELISA法检测血清GLP-1水平; 对各组大鼠进行16S-rDNA肠道菌群测序; RT-PCR法检测大鼠肝脏组织葡萄糖激酶(GCK)mRNA及脂肪组织葡萄糖转运体(GLUT4)mRNA表达。结果:与空白组比较,模型组大鼠FBG、HOMA-IR、拟杆菌门丰度、普氏菌属丰度、瘤胃球菌属丰度明显升高,血清GCK、GLUT4 mRNA及GLP-1表达水平、厚壁菌门丰度、厚壁菌门/拟杆菌门比值、乳酸杆菌属丰度、密螺旋体属丰度明显降低(均P<0.05); 与模型组比较,低剂量组、中剂量组、高剂量组、阳性对照组和Exendin-4组大鼠FBG、HOMA-IR、拟杆菌门丰度、普氏菌属丰度、瘤胃球菌属丰度明显降低,血清GCK、GLUT4 mRNA及GLP-1表达水平,厚壁菌门丰度、厚壁菌门/拟杆菌门比值、乳酸杆菌属丰度、密螺旋体属丰度明显升高(均P<0.05); 与高剂量组比较,高剂量组+Exendin-4组大鼠FBG、HOMA-IR、拟杆菌门丰度、普氏菌属丰度、瘤胃球菌属丰度明显降低,血清GCK、GLUT4 mRNA及GLP-1表达水平、厚壁菌门丰度、厚壁菌门/拟杆菌门比值、乳酸杆菌属丰度、密螺旋体属丰度明显升高(均P<0.05)。结论:麦冬多糖可通过调控GLP-1水平改善妊娠期糖尿病大鼠肠道菌群失调和糖代谢。
Abstract:
Objective:To investigate the effects of ophiopogon polysaccharides on intestinal flora and glucose metabolism in gestational diabetic rats by regulating GLP-1.Methods:Eighty pregnant rats were randomly divided into blank group,model group,low-dose group,medium-dose group,high-dose group,positive control group,Exendin-4 group and high dose + Exendin-4 group,10 rats in each group.After 2 weeks of intervention,FBG and HOMA-IR were compared among all groups.Serum GLP-1 levels were determined by ELISA.16S-rDNA intestinal flora was sequenced in each group.the expression of GCK and GLUT4 mRNA was detected by RT-PCR.Results:Compared with the blank group,the FBG,HOMA-IR,the abundance of Bacteroidets,Prevotella,Ruminococcus were significantly higher,and the serum GLP-1 level,GCK,GLUT4 mRNA expression,Firmicutes/Bacteroidetes ratio,the abundance of Firmicutes,Lactobacillus,Treponema were significantly lower in the model group(all P<0.05).Compared with the model group,FBG,HOMA-IR,the abundance of Bacteroidets,Prevotella,Ruminococcus were significantly lower in the low-dose group,medium-dose group,high-dose group,positive control group and Exendin-4 group,and serum GLP-1 level,GCK,GLUT4 mRNA expression,Firmicutes/Bacteroidetes ratio,the abundance of Firmicutes,Lactobacillus,Treponema were significantly higher(all P<0.05).Compared with the high-dose group,FBG,HOMA-IR,the abundance of Bacteroidets,Prevotella,Ruminococcus were significantly lower in the high-dose + Exendin-4 group,and the serum levels of GLP-1,GCK,GLUT4 mRNA expression,Firmicutes/Bacteroidetes ratio,the abundance of Firmicutes,Lactobacillus,Treponema were significantly higher(all P<0.05).Conclusion:Ophiopogon polysaccharide could improve the dysregulation of intestinal flora in gestational diabetic rats by regulating GLP-1 levels,and also had a significant improvement on glucose metabolism.

参考文献/References:

[1] 刘秋杨,朱慧芳,王迎春,等.黄芪四君子汤联合门冬胰岛素治疗妊娠期糖尿病临床研究[J].陕西中医,2021,42(10):1408-1411.
[2] 蔡红霞,丁金玉,张明恩.四君子汤对妊娠期糖尿病患者血管内皮功能及妊娠结局的影响[J].陕西中医,2020,41(12):1078-1711.
[3] 都 燕,任 娟,罗苗苗.肌醇和维生素B、D对妊娠期糖尿病小鼠炎症及脂代谢的影响及机制探讨[J].陕西医学杂志,2022,51(2):159-163.
[4] Dluski DF,Wolnska E,Skrzypczak M.Epigenetic changes in gestational diabetes mellitus[J].International Journal of Molecular Sciences,2021,22(14):7649.
[5] Ruszala M,Niebrzydowska M,Pilszyk A,et al.Novel biomolecules in the pathogenesis of gestational diabetes mellitus[J].International Journal of Molecular Sciences,2021,22(21):11578.
[6] 陈 静,张 晶,宋海宁.探讨麦冬多糖对糖尿病患者血糖及胰岛素抵抗的影响[J].世界最新医学信息文摘,2015,15(78):107-108.
[7] 宋 娜,苏东峰,刘晓燕,等.麦冬多糖对糖尿病围绝经期大鼠血清SOD、GSH-Px、CAT、MDA水平的影响[J].东南大学学报:医学版,2019,38(6):979-984.
[8] 马 刚,孙家忠.GLP-1 RAs联合恩格列净对2型糖尿病患者的治疗效果及对胰岛素抵抗的影响[J].实用医学杂志,2020,36(18):2500-2504.
[9] 姜 露,厉 平.GLP-1类似物和受体激动剂对2型糖尿病合并肥胖及单纯肥胖患者体重影响的研究进展[J].医学综述,2020,26(2):341-345.
[10] 孙 昕,加勒哈斯别克?塞力克,王艳明,等.乳源性复合益生菌对2型糖尿病大鼠GLP-1的影响及调控机制[J].中国微生态学杂志,2020,32(4):384-391.
[11] 彭俊华,葛锁华,张欢妍,等.参苓白术散联合二甲双胍对2型糖尿病肥胖者miR-146a、GLP-1及血脂的影响[J].天津医药,2021,49(2):203-207.
[12] 王余娜,高显舜,姚可荣.妊娠期糖尿病患者血清维生素D、白介素-17水平与胰岛素抵抗指数相关性研究[J].陕西医学杂志,2020,49(11):1429-1431,1439.
[13] Nuzzo AM,Giuffrida D,Moretti L,et al.Placental and maternal sFlt1/PlGF expression in gestational diabetes mellitus[J].Scientific Reports,2021,11(1):2312.
[14] Sililas P,Huang L,Thonusin C,et al.Association between gut microbiota and development of gestational diabetes mellitus[J].Microorganisms,2021,9(8):1686.
[15] 席啸虎.基于网络药理学的麦冬治疗糖尿病成分和机制及其配伍后作用通路改变研究[J].中国现代中药,2021,23(4):638-644.
[16] 邓 斌.2型糖尿病合并高血压患者应用麦冬汤合牛膝饮加味治疗的效果研究[J].基层医学论坛,2020,24(5):697-698.
[17] 廖 靖,蔡东红.麦冬多糖对妊娠期糖尿病小鼠胰岛抵抗作用的影响[J].药学研究,2011,30(8):435-437.
[18] 李 玥,杨晓玲,马 亮,等.GLP-1受体激动剂联合益生菌对糖尿病患者肠道菌群、氧化应激和糖脂代谢的影响[J].河北医药,2021,43(4):522-526.
[19] 黄凤连,袁慧娟.肠道菌群与糖尿病微血管病变关系的研究进展[J].中华糖尿病杂志,2022,14(3):282-285.
[20] 王 瑾,冉 倩,王 琳,等.基于16S rRNA技术分析黄连酒蒸前后对正常及2型糖尿病大鼠肠道微生物的影响[J].中国实验方剂学杂志,2019,25(22):92-102.
[21] 欧 杨,周怡昆.青少年发病的成人型糖尿病的妊娠期管理[J].国际内分泌代谢杂志,2021,41(5):422-425.

相似文献/References:

[1]董巍巍,段雪涛,王红红,等.六味地黄汤加味联合穴位按摩治疗妊娠期糖尿病疗效及对患者妊娠结局的影响*[J].陕西中医,2020,(4):447.
[2]刘秋杨,朱慧芳,王迎春,等.黄芪四君子汤联合门冬胰岛素治疗妊娠期糖尿病临床研究[J].陕西中医,2021,(10):1408.[doi:DOI:10.3969/j.issn.1000-7369.2020.10.021]
[3]袁建娜,张华俊,张瑞杰,等.杞菊地黄汤加减联合胰岛素治疗妊娠期糖尿病效果及对患者胰岛功能、脂肪因子和母婴结局的影响[J].陕西中医,2024,(8):1078.[doi:DOI:10.3969/j.issn.1000-7369.2024.08.015]
　YUAN Jianna,ZHANG Huajun,ZHANG Ruijie,et al.Effect of Qiju Dihuang decoction combined with insulin in treatment of gestational diabetes mellitus and its influence on islet function,adipokine and maternal and infant outcomes[J].,2024,(4):1078.[doi:DOI:10.3969/j.issn.1000-7369.2024.08.015]

备注/Memo

备注/Memo:
基金项目:河北省卫生健康委员会科研基金资助项目(20201472)
更新日期/Last Update: 2023-04-10