[1]刘华亭,郑博文,陈 晨,等.基于NLRP3/Caspase-1/IL-1β信号通路探讨复方蛇龙胶囊对膜性肾病大鼠的作用[J].陕西中医,2023,(6):709-716.[doi:DOI:10.3969/j.issn.1000-7369.2023.06.006]
　LIU Huating,ZHENG Bowen,CHEN Chen,et al.Effect of compound Shelong capsule on membranous nephropathy rats based on NLRP3/Caspase-1/IL-1β signaling pathway[J].,2023,(6):709-716.[doi:DOI:10.3969/j.issn.1000-7369.2023.06.006]
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基于NLRP3/Caspase-1/IL-1β信号通路探讨复方蛇龙胶囊对膜性肾病大鼠的作用
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年6期
页码:
709-716
栏目:
基础研究
出版日期:
2023-06-05

文章信息/Info

Title:
Effect of compound Shelong capsule on membranous nephropathy rats based on NLRP3/Caspase-1/IL-1β signaling pathway
作者:
刘华亭1; 郑博文2; 陈 晨1; 司瑞花1; 侯彦婕2; 刘光珍2
(1.山西中医药大学,山西 晋中 030002; 2.山西省中医药研究院,山西 太原 030012)
Author(s):
LIU Huating; ZHENG Bowen; CHEN Chen; SI Ruihua; HOU Yanjie; LIU Guangzhen
(Shanxi University of Chinese Medicine,Jinzhong 030002,China)
关键词:
膜性肾病;  复方蛇龙胶囊;  细胞焦亡;  白细胞介素;  半胱氨酸天冬氨酸蛋白水解酶-1
Keywords:
Membranous nephropathy;  Compound Shelong capsule;  Pyroptosis;  Interleukin;  Caspase-1
分类号:
R 692
DOI:
DOI:10.3969/j.issn.1000-7369.2023.06.006
文献标志码:
A
摘要:
目的:观察复方蛇龙胶囊对膜性肾病大鼠核苷酸结合域样受体蛋白3(NLRP3)/半胱氨酸天冬氨酸蛋白水解酶-1(Caspase-1)/白细胞介素(IL -1β)细胞焦亡信号通路的作用机制。方法:SD雄性大鼠分为正常组,模型组,复方蛇龙胶囊高、中、低剂量组,雷公藤多苷片组。给药结束后检测大鼠24 h尿蛋白(24 h-UTP)和血清总蛋白(TP)、血清白蛋白(ALB)、血肌酐(Scr)、尿素氮(BUN)、总胆固醇(TC)。马松(Masson)染色和电镜观察大鼠病理和足细胞超微结构; ELISA检测大鼠血清IL-1β、IL-18水平; 荧光定量PCR和免疫组化法(IHC)检测肾脏组织中NLRP3、 Caspase-1、IL-1β、GSDMD的mRNA和蛋白表达水平。结果:病理显示肾脏纤维化明显; 足突融合变宽,肾小球基底膜增厚; 与模型组相比较,各给药组TP、ALB、Scr、BUN、TC、24 h-UTP及IL-1β、IL-18水平明显降低(P<0.05),NLRP3、 Caspase-1、IL-1β、GSDMD的mRNA和蛋白表达水平明显降低(P<0.05)。结论:复方蛇龙胶囊可能通过抑制NLRP3/Caspase-1/IL-1β改善细胞焦亡从而减轻膜性肾病炎症损伤。
Abstract:
Objective:To observe the mechanism of compound Shelong capsule on the pyrogenic signaling pathway of nucleotide binding domain like receptor protein 3(NLRP3)/cysteine aspartic proteolytic enzyme 1(Caspase-1)/interleukin(IL-1β)in rats with membranous nephropathy.Methods:SD male rats were divided into normal group,model group,compound Shelong capsule high-dose,medium-dose and low-dose group,tripterygium wilfordii polyglycoside tablet group.The 24 h-UTP and serum TP,ALB,Scr,BUN and TC were detected after administration.The pathology and ultrastructure of podocytocytes were observed by Masson and electron microscope.Serum levels of IL-1β and IL-18 were determined by ELISA.The mRNA and protein expression levels of NLRP3,Caspase-1,IL-1β and GSDMD in renal tissues were detected by Real-time PCR and IHC.Results:Pathology showed obvious renal fibrosis.The fusion of the foot process widened and the basement membrane thickened.Compared with model group,the levels of TP,ALB,Scr,BUN,TC,24 h-UTP,IL-1β and IL-18 in each administration group were significantly decreased(P<0.05),and the mRNA and protein expression levels of NLRP3,Caspase-1,IL-1β and GSDMD were significantly decreased(P<0.05).Conclusion:Compound Shelong capsule may reduce the inflammatory damage of membranous nephropathy by inhibiting NLRP3/Caspase-1/IL-1β.

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备注/Memo

备注/Memo:
基金项目:山西省重点研发计划(指南)项目(201703D421024); 山西省中医药管理局科研课题(2022ZYYZ001); 山西省中医药研究院中药新药研究专项课题(2016XYZX02); 山西省中医药研究院院级科研项目(202216)
更新日期/Last Update: 2023-06-09