[1]袁海阳,朱梦姚,王 祎,等.黄芪甲苷对记忆损伤小鼠行为学及海马体的影响[J].陕西中医,2023,(7):843-848.[doi:DOI:10.3969/j.issn.1000-7369.2023.07.004]
　YUAN Haiyang,ZHU Mengyao,WANG Yi,et al.Effects of astragaloside Ⅳ on behavior and hippocampus of mice with memory impairment[J].,2023,(7):843-848.[doi:DOI:10.3969/j.issn.1000-7369.2023.07.004]
点击复制

黄芪甲苷对记忆损伤小鼠行为学及海马体的影响
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年7期
页码:
843-848
栏目:
基础研究
出版日期:
2023-07-05

文章信息/Info

Title:
Effects of astragaloside Ⅳ on behavior and hippocampus of mice with memory impairment
作者:
袁海阳1; 朱梦姚1; 王 祎1; 郭自贺1; 吕 馨1; 蒋蒙蒙1; 贡岳松1; 2
(1.南京中医药大学,江苏 南京 210023; 2.江苏鹏鹞药业新药创新中心,江苏 宜兴 214205)
Author(s):
YUAN Haiyang; ZHU Mengyao; WANG Yi; GUO Zihe; LYU Xin; JIANG Mengmeng; GONG Yuesong
(Nanjing University of Chinese Medicine,Nanjing 210023,China)
关键词:
黄芪甲苷;  链脲佐菌素;  记忆损伤;  海马体;  行为学;  突触
Keywords:
Astragaloside Ⅳ;  Streptozotocin;  Memory impairment;  Hippocampal body;  Behavior science;  Synapse
分类号:
R 282
DOI:
DOI:10.3969/j.issn.1000-7369.2023.07.004
文献标志码:
A
摘要:
目的:研究黄芪甲苷对于单侧脑室注射链脲佐菌素小鼠的行为学以及海马体影响。方法:将链脲佐菌素(STZ)注射于单侧脑室的小鼠随机分为模型组、黄芪甲苷高剂量组、黄芪甲苷中剂量组、黄芪甲苷低剂量组、多奈哌齐组,另设对照组、假手术组。灌胃给药30 d后,行为学实验检测小鼠空间学习记忆能力。试剂盒检测血清中超氧化物歧化酶抑制率,海马体中丙二醛以及乙酰胆碱酯酶活力。苏木精-伊红染色法检测小鼠大脑海马CA1区病理状态。蛋白印迹法检测小鼠海马体中NR2A、NR2B、Shank3、Psd95、PI3K、p-Akt、Akt蛋白表达情况。结果:黄芪甲苷高剂量组逃避潜伏期短于模型组,差异有统计学意义(P<0.01)。黄芪甲苷高剂量组血清超氧化物歧化酶抑制率、海马体中乙酰胆碱酯酶、丙二醛活力下降低于模型组,差异有统计学意义(均P<0.01)。黄芪甲苷高剂量组海马体Psd95、PI3K、NR2A、p-Akt、Shank3、NR2B表达高于模型组,差异有统计学意义(均P<0.05)。结论:黄芪甲苷可上调单侧脑室注射STZ小鼠海马体中NR2A、NR2B、Shank3、Psd95、PI3K、p-Akt表达,减轻海马体中氧化应激损伤,改善小鼠行为学。
Abstract:
Objective:To study effect of astragaloside on behavior and hippocampus of mice injected with streptozotocin into unilateral ventricle.Methods:The mice injected STZ into unilateral ventricle were randomly divided into model group,high dose astragaloside group,middle dose astragaloside group,low dose astragaloside group,Donepezil group,control group and sham operation group.After intragastric administration for 30 days,the spatial learning and memory ability of mice were tested by behavioral experiment.The inhibition rate of superoxide dismutase in serum and activity of malondialdehyde and acetylcholinesterase in hippocampus were detected by kit.Hematoxylin-eosin staining was used to detect pathological status of hippocampal CA1 region in mice.Western blotting was used to detect the expression of NR2A,NR2B,Shank3,Psd95,PI3K,p-Akt and Akt in the hippocampus of mice.Results:The escape latency of the high dose astragaloside group was shorter than that in the model group,difference statistically significant(P<0.01).The inhibition rate of serum superoxide dismutase,the activity of acetylcholinesterase and malondialdehyde in hippocampus of the high dose astragaloside group were lower than those in the model group,difference statistically significant(all P<0.01).The expressions of Psd95,PI3K,NR2A,p-Akt,Shank3 and NR2B in the hippocampus of the high dose astragaloside group were higher than those in the model group,difference statistically significant(all P<0.01).Conclusion:Astragaloside can up-regulate the expression of NR2A,NR2B,Shank3,Psd95,PI3K and p-Akt in the hippocampus of STZ mice injected into unilateral ventricle,reduce oxidative stress injury in the hippocampus and improve behavior of mice.

参考文献/References:

[1] Lane CA,Hardy J,Schott JM.Alzheimer's disease[J].European Journal of Neurology,2018,25(1):59-70.
[2] 谢喆尧,张 希,杨浩辰,等.中医从五脏论治阿尔茨海默病的研究进展[J].湖北民族大学学报,2023,40(1):76-80.
[3] 杜武勋,柴山周乃,魏聪聪.张伯礼治疗轻度认知功能障碍和老年痴呆的经验[J].辽宁中医杂志,2012,39(7):1225-1228.
[4] 郝文杰,江海林,杨文明,等.补肾益髓法治疗阿尔茨海默病的Meta分析[J].中国民族民间医药,2022,31(15):111-118.
[5] 李 慧,刘文婷,刘廷辉,等.中医药治疗阿尔茨海默病研究进展[J].河北中医,2022,44(9):1558-1562.
[6] 张瑞华,张静文,刘 玲,等.黄芪及其有效组分药理作用与临床应用现状[J].陕西中医,2021,42(8):1138-1141,1146.
[7] 黎金浓,何娅娜,周 辉,等.基于网络药理学的黄芪抗肺纤维化机制预测与验证研究[J].湖南中医药大学学报,2022,42(2):278-284.
[8] Zhang JQ,Wu CX,Gao L,et al.Astragaloside Ⅳderived from astragalus membranaceus:A research review on the pharmacological effects[J].Advances in Pharmacology,2020,42(87):89-112.
[9] Chang CP,Liu YF,Lin HJ,et al.Beneficial effect of astragaloside on Alzheimer's disease condition using cultured primary cortical cells under β-amyloid exposure[J].Molecular Neurobiology,2016,53(10):7329-7340.
[10] Wang X,Wang Y,Hu JP,et al.Astragaloside Ⅳ a natural PPARγ agonist reduces Aβ production in Alzheimer's disease through inhibition of Bace-1[J].Molecular Neurobiology,2017,54(4):2939-2949.
[11] 张利平,彭 静.清心益智汤对于改善阿尔茨海默病患者生活质量、认知水平以及SOD、MDA等生化指标的作用分析[J].陕西中医,2018,39(5):593-595.
[12] 梁瑞景,梁瑞凯.黄连解毒汤联合复方苁蓉益智胶囊、多奈哌齐片对阿尔茨海默病患者尿AD相关神经丝蛋白水平的影响[J].陕西中医,2021,42(4):446-448,462.
[13] Li NN,Feng FF,Wu K,et al.Inhibitory effects of astragaloside Ⅳ on silica-induced pulmonary fibrosis via inactivating TGF-β1/Smad3 signaling[J].Biomedicine & Pharmacotherapy,2019,11(9):109387.
[14] 晏 燕.黄芪主要有效成分对Aβ诱导阿尔茨海默病模型的干预及氧化机制研究[D].武汉:武汉大学,2016.
[15] Hsieh HL,Liu SH,Chen YL,et al.Astragaloside Ⅳ suppresses inflammatory response via suppression of NF-κB and MAPK signalling in human bronchial epithelial cells[J].Archives of Physiology and Biochemistry,2020,128(3):757-766.
[16] Niu YC,Chen YF,Xu HY,et al.Astragaloside Ⅳ promotes antiphotoaging by enhancing the proliferation and paracrine activity of adipose-derived stem cells[J].Stem Cells and Development,2020,29(19):1285-1293.
[17] Xing L,Fang J,Zhu BB,et al.Astragaloside Ⅳ protects against podocyte apoptosis by inhibiting oxidative stress via activating PPARγ-Klotho-FoxO1 axis in diabetic nephropathy[J].Life Sciences,2021,26(9):119068.
[18] Alkadhi KA.Cellular and molecular differences between area CA1 and the dentate gyrus of the hippocampus[J].Molecular Neurobiology,2019,56(9):6566-6580.
[19] Mi SX,Chen H,Lin PJ,et al.Camkll is a modulator in neurodegenerative diseases and mediates the effect of and rogen on synaptic protein Psd95[J].Frontiers in Genetics,2022,4(13):959360.
[20] Lutz AK,Bauer HF,Loannids V,et al.Shank3 antibody validation:Differential performance in western blotting immunocyto and immunohistochemistry[J].Front Synaptic Neurosci,2022,6(14):890231.
[21] 王 洁,吴子建,蔡荣林,等.电针“心俞-神门”对急性心肌缺血模型小鼠心肌与海马组织Glu、Asp和NR1表达的影响[J].湖南中医药大学学报,2021,41(1):79-84.
[22] Ding MR,Qu YJ,Hu B,et al.Signal pathways in the treatment of Alzheimers' disease with traditional Chinese medicine[J].Biomed Pharmacother,2022,8(152):113208.
[23] Sun Y,Zhang HL,Wu ZJ,et al.Quercitrin rapidly alleviated depression-like behaviors in lipopolysaccharide-treated mice:The involvement of PI3K/Akt/NF-κB signaling suppression and Creb/Bdnf signaling restoration in the hippocampus[J].ACS Chemical Neuroscience,2021,12(18):3387-3396.
[24] Lin JY,Kuo WW,Baskaran R,et al.Swimming exercise stimulates IGF1/PI3K/Akt and AMPK/SIRT1/PGC1α survival signaling to suppress apoptosis and inflammation in aging hippocampus[J].Aging,2020,12(8):6852-6864.

相似文献/References:

[1]徐 智,张友志△,郭昌洪,等.益气化瘀口服液质量标准研究*[J].陕西中医,2019,(9):1304.
[2]马红静,马民凯,马红建,等.通络散结汤对2型糖尿病大鼠调节性T细胞及炎症因子的影响[J].陕西中医,2021,(4):423.[doi:DOI:10.3969/j.issn.1000-7369.2020.04.004]
　MA Hongjing,MA Minkai,MA Hongjian,et al.Effects of Tongluo Sanjie decoction on T regulatory cells and inflammatory factors in type 2 diabetic rats[J].,2021,(7):423.[doi:DOI:10.3969/j.issn.1000-7369.2020.04.004]

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81603383)
更新日期/Last Update: 2023-07-10