[1]李丽琴,张 鼎,袁 莉,等.温阳复元方联合miR-137模拟剂调控线粒体铁死亡对脑缺血再灌注损伤大鼠的作用机制[J].陕西中医,2023,(10):1354-1359.[doi:DOI:10.3969/j.issn.1000-7369.2023.10.005]
　LI Liqin,ZHANG Ding,YUAN Li,et al.Effect mechanism of Wenyang Fuyuan formula combined with miR-137 mimic to regulate mitochondrial iron death in rats with cerebral ischemia-reperfusion injury[J].,2023,(10):1354-1359.[doi:DOI:10.3969/j.issn.1000-7369.2023.10.005]
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温阳复元方联合miR-137模拟剂调控线粒体铁死亡对脑缺血再灌注损伤大鼠的作用机制
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年10期
页码:
1354-1359
栏目:
基础研究
出版日期:
2023-10-05

文章信息/Info

Title:
Effect mechanism of Wenyang Fuyuan formula combined with miR-137 mimic to regulate mitochondrial iron death in rats with cerebral ischemia-reperfusion injury
作者:
李丽琴1; 张 鼎1; 袁 莉1; 向军军2; 陈 炜2; 胡跃强2
(1.广西中医药大学,广西 南宁 530001; 2.广西中医药大学第一附属医院,广西 南宁 530001)
Author(s):
LI Liqin; ZHANG Ding; YUAN Li; XIANG Junjun; CHEN Wei; HU Yueqiang
(Guangxi University of Chinese Medicine,Nanning 530001,China)
关键词:
脑缺血再灌注损伤;  温阳复元方;  缺血性中风;  线粒体铁死亡;  神经功能缺损评分;  miR-137模拟剂
Keywords:
Ischemia reperfusion injury;  Wenyang Fuyuan formula;  Ischemic stroke;  Mitochondrial iron death;  Neurological deficit scores;  miR-137 mimic
分类号:
R 743.31
DOI:
DOI:10.3969/j.issn.1000-7369.2023.10.005
文献标志码:
A
摘要:
目的:阐明大鼠脑缺血再灌注(I/R)后miR-137对转铁蛋白受体(TFR)和胸腺癌抵抗蛋白(BCRP)表达的调控作用及温阳复元方的干预效应。方法:75只SD大鼠分为五组,假手术组和模型组予0.9%氯化钠溶液灌胃,余组予温阳复元方灌胃,miR-137模拟组和miR-137模拟对照组大鼠使用脑立体定位法分别将miR-137模拟剂和miR-137空载体注射到缺血侧MCA区,成模后再灌注24 h、7 d、14 d大鼠取材; 评价各组大鼠的神经功能缺损评分以及TTC染色; 分别通过RT-qPCR、Western blot检测各组大鼠脑组织中相关蛋白和mRNA的表达情况。结果:与假手术组相比,模型组大鼠神经功能缺陷评分较高(P<0.01); 成模后大鼠脑组织出现明显的白色梗死灶。与模型组比较,温阳复元方组、miR-137模拟组和miR-137模拟对照组大鼠脑组织中miR-137以及TFR mRNA和蛋白表达量均明显降低,其中miR-137模拟组降低最为显著(P<0.01); 同时,大鼠脑组织中BCRP mRNA和蛋白表达均明显升高,其中miR-137模拟组升高最为明显(P<0.01)。结论:miR-137可能通过下调TFR、上调BCRP的表达,从而达到对大鼠神经元的保护作用,温阳复元方能进一步增强其保护作用。
Abstract:
Objective:To elucidate the regulatory effects of miR-137 on the expression of transferrin receptor(TFR)and thymic carcinoma resistance protein(BCRP)after cerebral ischemia/reperfusion(I/R)in rats and the intervention effect of Wenyang Fuyuan formula.Methods:Seventy-five SD rats were divided into five groups:the sham-operated group and the model group were given 0.9% NaCl solution by gavage,and the remaining group was given Wenyang Fuyuan formula by gavage.miR-137 mimic and miR-137 mimic control rats were injected with miR-137 mimic and miR-137 empty vector into the ischemic side of the MCA area using the brain stereotaxic method,and then perfused for 24 h,7 d,and 14 d after model formation.The rats were sacrificed at 14 d for sampling; the neurological deficit scores and TTC staining of each group were evaluated; the expression of relevant proteins and mRNAs in the brain tissue of each group were detected by RT-qPCR and Western blot,respectively.Results:Compared with the sham-operated group,the neurological function deficit score was higher in the model group rats(P<0.01); the brain tissues of rats showed obvious white infarct foci after modeling.Compared with the model group,the expression of miR-137 and TFR mRNA and protein in the brain tissue of rats in the Wenyang Fuyuan formula group,miR-137 mimic group and miR-137 mimic control group were significantly reduced,with the most significant reduction in the miR-137 mimic group(P<0.01); meanwhile,the expression of BCRP mRNA and protein in the brain tissue of rats were significantly increased,with the most significant increase in the miR-137 mimic group.The expression of BCRP mRNA and protein in rat brain tissues was significantly increased,with the most significant increase in the miR-137 mimic group(P<0.01).Conclusion:miR-137 may play a neuronal protective role by down-regulating TFR and up-regulating the expression of BCRP,and its protective effect can be further enhanced by Wenyang Fuyuan formula.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81973768); 广西自然科学基金资助重点项目(2019GXNSFDA245006); 广西中医脑病临床研究中心项目(桂科AD20238028)
更新日期/Last Update: 2023-10-09