[1]李东光,于 波,李笑莹,等.刺五加多糖对骨肉瘤小鼠抑瘤作用及免疫功能的影响[J].陕西中医,2023,(10):1372-1376.[doi:DOI:10.3969/j.issn.1000-7369.2023.10.008]
　LI Dongguang,YU Bo,LI Xiaoying,et al.The anti-tumor effect of acanthopanax senticosus polysaccharides on osteosarcoma mice and its impact on immune function[J].,2023,(10):1372-1376.[doi:DOI:10.3969/j.issn.1000-7369.2023.10.008]
点击复制

刺五加多糖对骨肉瘤小鼠抑瘤作用及免疫功能的影响
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年10期
页码:
1372-1376
栏目:
基础研究
出版日期:
2023-10-05

文章信息/Info

Title:
The anti-tumor effect of acanthopanax senticosus polysaccharides on osteosarcoma mice and its impact on immune function
作者:
李东光; 于 波; 李笑莹; 曹 静; 王 禹
(佳木斯市中医医院,黑龙江 佳木斯 154002)
Author(s):
LI Dongguang; YU Bo; LI Xiaoying; CAO Jing; WANG Yu
(Jiamusi Hospital of Traditional Chinese Medicine,Jiamusi 154002,China)
关键词:
骨肉瘤;  刺五加多糖;  抑瘤作用;  免疫功能;  胸腺指数;  脾脏指数
Keywords:
Osteosarcoma;  Acanthopanax senticosus polysaccharides;  Antitumor effect;  Immune function;  Thymus index;  Spleen index
分类号:
R 738.1
DOI:
DOI:10.3969/j.issn.1000-7369.2023.10.008
文献标志码:
A
摘要:
目的:探讨刺五加多糖(ASPS)对骨肉瘤小鼠的抑瘤作用及对免疫功能的影响。方法:通过前肢后内侧注射MG63细胞悬液建立骨肉瘤小鼠模型; 将造模后的小鼠随机分为模型组、刺五加多糖低、中、高(36.25、72.5、145 mg/kg)剂量组、顺铂组(25 mg/kg顺铂),并以正常小鼠为对照组,每组10只; 干预结束后,ELISA检测血清中白介素-2(IL-2)、干扰素-γ(IFN-γ)以及肿瘤坏死因子-α(TNF-α)水平; 流式细胞仪检测外周血中CD4+、CD8+细胞比例; 分离胸腺及脾脏,计算胸腺指数和脾脏指数; 分离肿瘤,称量瘤体重量并计算抑瘤率; HE检测肿瘤组织病理学变化; Western blot检测肿瘤组织程序性死亡因子1(PD-1)及配体(PD-L1)蛋白表达。结果:与对照组相比,小鼠血清中IL-2、IFN-γ、TNF-α水平、外周血中CD8+、瘤体重量、肿瘤组织中PD-1、PD-L1蛋白表达显著增加,脾脏指数、胸腺指数、CD4+及CD4+/CD8+显著降低(P<0.05); 与模型组相比,刺五加多糖低、中、高剂量组部分细胞核分裂,肿瘤细胞减少,血清中IL-2、IFN-γ、TNF-α水平、外周血中CD8+、瘤体重量、肿瘤组织中PD-1、PD-L1蛋白表达均显著降低,脾脏指数、胸腺指数、CD4+及CD4+/CD8+、抑瘤率均显著增加(P<0.05),但顺铂组与刺五加多糖高剂量组比较差异无统计学意义(P>0.05)。结论:ASPS可抑制骨肉瘤小鼠肿瘤生长,改善小鼠免疫功能。
Abstract:
Objective:To investigate the anti-tumor effect of acanthopanax senticosus polysaccharides(ASPS)on osteosarcoma mice and its impact on immune function.Methods:A mouse model of osteosarcoma was established by injecting MG63 cell suspension into the posterior medial side of the forelimbs; the model mice were randomly divided into model group,Acanthopanax senticosus polysaccharide low(36.25 mg/kg),medium(72.5 mg/kg),high(145 mg/kg)dose groups,and cisplatin group(25 mg/kg cisplatin),with normal mice as the control group,with 10 mice in each group; after the intervention,ELISA was applied to detect serum levels of IL-2,IFN-γ,and TNF-α; flow cytometry was applied to detect the proportions of CD4+ and CD8+ cells in peripheral blood; the thymus and spleen were separated,and the thymus index and spleen index were calculated; the tumor was separated,the tumor mass was weighed,and the tumor inhibition rate was calculated; HE was applied to detect histopathology changes of tumors; Western blot was applied to detect the expression of programmed death factor-1(PD-1)and ligand(PD-L1)proteins in tumor tissue.Results:Compared with the control group,the levels of IL-2,IFN-γ,TNF-α in mouse serum,CD8+ in peripheral blood,tumor mass,and the expression of PD-1 and PD-L1 proteins in tumor tissue were obviously increased,the spleen index,thymus index,CD4+ and CD4+/CD8+ were obviously decreased(P<0.05).Compared with the model group,the Acanthopanax senticosus polysaccharide low-dose group,medium-dose group,high-dose group showed partial nuclear division and reduced tumor cells,the levels of IL-2,IFN-γ,TNF-α in serum,CD8+ in peripheral blood,tumor mass,and the expression of PD-1 and PD-L1 proteins in tumor tissue were obviously decreased,the spleen index,thymus index,CD4+,CD4+/CD8+,and tumor inhibition rate were obviously decreased(P<0.05).However,there was no obvious difference between the cisplatin group and the Acanthopanax senticosus polysaccharide high-dose group(P>0.05).Conclusion:ASPS can inhibit tumor growth and improve immune function in osteosarcoma mice.

参考文献/References:

[1] Ren L,Huang S,Beck J,et al.Impact of limb amputation and cisplatin chemotherapy on metastatic progression in mouse models of osteosarcoma[J].Sci Rep,2021,11(1):24435.
[2] Shen Y,Zhao S,Wang S,et al.S1P/S1PR3 axis promotes aerobic glycolysis by YAP/c-MYC/PGAM1 axis in osteosarcoma[J].Ebio Medicine,2019,40:210-223.
[3] 陈 伟,刘海生,魏 萌,等.熊果酸对荷骨肉瘤大鼠免疫功能的影响[J].环球中医药,2017,10(3):279-283.
[4] 徐建立,孙艳华,李 明,等.紫草素通过抑制上皮间质转化缓解结肠癌细胞5-氟尿嘧啶耐药[J].陕西中医,2022,43(3):296-300.
[5] 王 莹,牟琪瑞,刘春英,等.补中益气汤联合顺铂对肺腺癌荷瘤裸鼠移植瘤连环蛋白mRNA及蛋白表达的影响[J].中华中医药杂志,2022,37(4):2191-2194.
[6] 孟庆龙,刘雅婧,崔文玉,等.刺五加多糖抗炎作用的研究[J].时珍国医国药,2019,30(11):2621-2624.
[7] Sun D,Chen J,Hu H,et al.Acanthopanax senticosus polysaccharide suppressing proliferation and metastasis of the human non-small cell lung cancer NCI-H520 cells is associated with Wnt/β-catenin signaling[J].Neoplasma,2019,66(4):555-563.
[8] 杨 健,张立喜,史 博.石见穿多糖通过PTEN通路对骨肉瘤小鼠的抑瘤作用及对免疫功能的影响[J].天津中医药,2022,39(7):940-944.
[9] 张 娜,赵良友,毛 迪,等.刺五加多糖调控炎性因子对小鼠免疫性肝损伤的保护作用[J].中国中药杂志,2019,44(14):2947-2952.
[10] 李 森,王 健,古钦文,等.寄生软化汤诱导骨肉瘤小鼠的免疫效应[J].世界中医药,2018,13(6):1518-1520,1524.
[11] Wu NF,Yamamoto J,Bouvet M,et al.A novel procedure for orthotopic tibia implantation for establishment of a more clinical osteosarcoma PDOX mouse model[J].In Vivo,2021,35(1):105-109.
[12] Corre I,Verrecchia F,Crenn V,et al.The osteosarcoma microenvironment:A complex but targetable ecosystem[J].Cells,2020,9(4):976.
[13] 刘怀毫,梁 振,梁沛杰,等.骨肉瘤化学治疗的研究进展[J].中国临床新医学,2018,11(3):311-314.
[14] Meng Q,Pan J,Liu Y,et al.Anti-tumour effects of polysaccharide extracted from acanthopanax senticosus and cell-mediated immunity[J].Exp Ther Med,2018,15(2):1694-1701.
[15] 陈靖昀,于书剑,肖 冬.刺五加多糖对肿瘤干细胞的作用研究[J].中国实验诊断学,2019,23(7):1233-1238.
[16] 辛欢欢,李春玲,王贵平.白花蛇舌草黄酮注射液对小鼠脾淋巴细胞的免疫增强作用[J].中国兽医科学,2010,40(7):752-757.
[17] 杜娜雯,白日兰,崔久嵬.肿瘤免疫逃逸机制及治疗策略[J].中国肿瘤生物治疗杂志,2019,26(4):454-462.
[18] 陈 玉,肖海娟,王惠玲,等.基于伏邪理论探讨恶性肿瘤发病机制[J].陕西中医,2022,43(12):1760-1762,1767.
[19] 郭家毓,呼 群.小细胞肺癌免疫治疗研究进展[J].陕西医学杂志,2023,52(1):118-121.
[20] Jiang W,Pan S,Chen X,et al.The role of lncRNAs and circRNAs in the PD-1/PD-L1 pathway in cancer immunotherapy[J].Mol Cancer,2021,20(1):116.
[21] Huang X,Zhang W,Zhang Z,et al.Prognostic value of programmed cell death 1 ligand-1(PD-L1)or PD-1 expression in patients with osteosarcoma:A meta-analysis[J].J Cancer,2018,9(14):2525-2531.
[22] 梁乐乐,王 鹏,刘俊霞,等.非小细胞肺癌组织中T细胞免疫球蛋白区黏蛋白-3、程序性死亡因子-1及程序性因子配体1的表达分析[J].陕西医学杂志,2021,50(10):1227-1230.
[23] Shimizu T,Fuchimoto Y,Okita H,et al.A curative treatment strategy using tumor debulking surgery combined with immune checkpoint inhibitors for advanced pediatric solid tumors:An in vivo study using a murine model of osteosarcoma[J].J Pediatr Surg,2018,53(12):2460-2464.
[24] Shimizu T,Fuchimoto Y,Fukuda K,et al.The effect of immune checkpoint inhibitors on lung metastases of osteosarcoma[J].J Pediatr Surg,2017,52(12):2047-2050.
[25] Zheng B,Ren T,Huang Y,et al.PD-1 axis expression in musculoskeletal tumors and antitumor effect of nivolumab in osteosarcoma model of humanized mouse[J].J Hematol Oncol,2018,11(1):16.

相似文献/References:

[1]李 晨,程联智,洪国勇,等.牛蒡子苷元对骨肉瘤细胞增殖和侵袭影响研究*[J].陕西中医,2019,(4):415.
　LI Chen,CHENG Lianzhi,HONG Guoyong,et al.Effect of arctigenin on proliferation and invasion of osteosarcoma cells[J].,2019,(10):415.

备注/Memo

备注/Memo:
基金项目:黑龙江省卫生健康委员会科研课题(20210404070401)
更新日期/Last Update: 2023-10-09