[1]梅苹苹,郭修田,冯文哲,等.加味生肌玉红膏对肛瘘模型大鼠术后创面的作用及血清炎症因子水平的影响[J].陕西中医,2024,(2):153-158.[doi:DOI:10.3969/j.issn.1000-7369.2024.02.002]
　MEI Pingping,GUO Xiutian,FENG Wenzhe,et al.Modified Shengji Yuhong ointment on wound model rats after anal fistula surgery and serum inflammatory factors[J].,2024,(2):153-158.[doi:DOI:10.3969/j.issn.1000-7369.2024.02.002]
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加味生肌玉红膏对肛瘘模型大鼠术后创面的作用及血清炎症因子水平的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年2期
页码:
153-158
栏目:
基础研究
出版日期:
2024-02-05

文章信息/Info

Title:
Modified Shengji Yuhong ointment on wound model rats after anal fistula surgery and serum inflammatory factors
作者:
梅苹苹1; 郭修田1; 冯文哲2; 林 璐2
(1.上海中医药大学附属市中医医院,上海 200071; 2.陕西中医药大学附属医院肛肠科,陕西 咸阳 712000)
Author(s):
MEI Pingping; GUO Xiutian; FENG Wenzhe; LIN Lu
(Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China)
关键词:
肛瘘;  加味生肌玉红膏;  炎症因子;  血管内皮生长因子;  创面;  大鼠
Keywords:
Anal fistula;  Modified Shengji Yuhong ointment;  Inflammatory factors;  Vascular endothelial growth factor;  Wound;  Rats
分类号:
R 266
DOI:
DOI:10.3969/j.issn.1000-7369.2024.02.002
文献标志码:
A
摘要:
目的:观察加味生肌玉红膏对肛瘘术后创面愈合、炎症因子及血管内皮生长因子表达水平的影响。方法:60只健康雄性大鼠分为凡士林组10只、康复新液组10只、加味生肌玉红膏组10只; 另外再设三组需在药物干预基础上注射核因子κB(NF-κB)信号通路抑制剂(PDTC),即PDTC+凡士林组、PDTC+康复新液组、PDTC+加味生肌玉红膏组,每组各10只。建立肛瘘术后大鼠创面模型并予不同方式干预。在第3、7、14天时分别观察记录大鼠创面愈合情况,并计算愈合率; 同时每只大鼠抽取尾静脉血液用于ELISA法检测炎症因子白介素1β(IL-1β)、白介素6(IL-6)、肿瘤坏死因子-α(TNF-α)及血管内皮生长因子(VEGF)表达情况。结果:在治疗的各个时间点,加味生肌玉红膏组与PDTC+加味生肌玉红膏组创面愈合率显著高于其余各组,差异有统计学意义(P<0.05); ELISA法检测显示,治疗各时间点,加味生肌玉红膏组与PDTC+加味生肌玉红膏组的IL-1β、TNF-α、IL-6表达水平均显著低于凡士林组与康复新液组(P<0.05),且PDTC+加味生肌玉红膏组IL-1β、TNF-α、IL-6表达水平均低于其余各组(P<0.05)。给药第3天,凡士林组的VEGF含量最低(P<0.05); 给药第7天,各组VEGF含量逐渐升高,PDTC+加味生肌玉红膏VEGF含量高于其余各组(P<0.05); 给药第14天,VEGF含量较第7天稍低,PDTC+加味生肌玉红膏与加味生肌玉红膏组VEGF表达含量比较差异无统计学意义(P>0.05),但高于其余各组(P<0.05)。结论:加味生肌玉红膏可通过NF-κB 信号通路来抑制 IL-1β、TNF-α、IL-6蛋白的表达,且可上调VEGF蛋白的表达,从而促进血管内皮细胞新生,减轻炎性反应,加速创面组织恢复,从而缩短愈合时间。
Abstract:
Objective:The study observed the impact of modified Shengji Yuhong ointment on wound healing,inflammatory factors,and expression of vascular endothelial growth factor after anal fistula surgery.Methods:Sixty healthy male rats were categorized into six groups:the Vaseline group(n=10),the Kangfuxin liquid group(n=10),and the modified Shengji Yuhong ointment group(n=10).Furthermore,the other three groups were injected with nuclear factor κB(NF-κB)signaling pathway inhibitors on the basis of drug intervention; namely the PDTC+ Vaseline group,PDTC + Kangfuxin liquid group,and PDTC+ modified Shengji Yuhong ointment,with 10 rats in each group.The rats underwent an anal fistula operation,following which a wound model was established and diverse interventions were administered.The wound healing of the rats was observed,recorded and the healing rate calculated at 3,7 and 14 days post-operation.Additionally,the expression of inflammatory factors such as interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and vascular endothelial growth factor(VEGF)were determined through ELISA.Results:At every stage of treatment,the rate of wound healing in the modified Shengji Yuhong ointment group and the PDTC + modified Shengji Yuhong ointment group was substantially greater than in each separate group.The disparity was statistically significant(P<0.05).The ELISA method revealed that the expression levels of IL-1β,TNF-α,and IL-6 in both the modified Shengji Yuhong ointment and PDTC + modified Shengji Yuhong ointment groups were significantly lower than those in the Vaseline group and the Kangfuxin liquid group at each time point of treatment(P<0.05).Additionally,the expression levels of IL-1β,TNF-α,and IL-6 in the PDTC + modified Shengji Yuhong ointment group were lower than those in each group(P<0.05).On the third day of administration,the VEGF content was found to be the lowest in the Vaseline group(P<0.05).The VEGF content of each group gradually increased on the seventh day,and PDTC + modified Shengji Yuhong ointment had a higher VEGF content compared to each group(P<0.05).On day 14 following administration,the level of VEGF was marginally reduced compared to day 7 substantial disparity in VEGF expression was evident between the groups receiving PDTC + modified Shengji Yuhong ointment and those administered modified Shengji Yuhong ointment(P>0.05).However,it was greater than each group individually(P<0.05).Conclusion:Modified Shengji Yuhong ointment is capable of suppressing the expression of IL-1β,TNF-α,IL-6 proteins through the NF-κB signalling pathway.Additionally,it can augment the production of VEGF proteins,thereby facilitating the regeneration of vascular endothelial cells,curbing inflammatory responses,hastening wound tissue recovery,and shortening the healing duration.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81904208); 陕西省科技厅课题(2020JM-588)
更新日期/Last Update: 2024-02-07