[1]张 颖,靳亦龙,周立新.芪地糖肾颗粒对2型糖尿病大鼠胰岛素抵抗、脂代谢及AdipoR1、AMPK蛋白的影响[J].陕西中医,2024,(2):176-180.[doi:DOI:10.3969/j.issn.1000-7369.2024.02.006]
　ZHANG Ying,JIN Yilong,ZHOU Lixin.Effects of Qidi Tangshen granule on insulin resistance,lipid metabolism and AdipoR1,AMPK protein in type 2 diabetes mellitus rats[J].,2024,(2):176-180.[doi:DOI:10.3969/j.issn.1000-7369.2024.02.006]
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芪地糖肾颗粒对2型糖尿病大鼠胰岛素抵抗、脂代谢及AdipoR1、AMPK蛋白的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年2期
页码:
176-180
栏目:
基础研究
出版日期:
2024-02-05

文章信息/Info

Title:
Effects of Qidi Tangshen granule on insulin resistance,lipid metabolism and AdipoR1,AMPK protein in type 2 diabetes mellitus rats
作者:
张 颖1; 靳亦龙2; 周立新1
(1.衡水市人民医院内分泌科,河北 衡水 053000; 2.衡水市第四医院,河北 衡水 053000)
Author(s):
ZHANG Ying; JIN Yilong; ZHOU Lixin
(Endocrine Department,People's Hospital of Hengshui,Hengshui 053000,China)
关键词:
2型糖尿病;  芪地糖肾颗粒;  胰岛素抵抗;  脂代谢;  脂联素受体1;  腺苷酸激活蛋白激酶
Keywords:
Type 2 diabetes mellitus;  Qidi Tangshen granules;  Insulin resistance;  Lipid metabolism;  Adiponectin receptor1;  AMP-activated protein kinase
分类号:
R 587
DOI:
DOI:10.3969/j.issn.1000-7369.2024.02.006
文献标志码:
A
摘要:
目的:探究芪地糖肾颗粒对2型糖尿病大鼠胰岛素抵抗、脂代谢以及脂联素受体1(AdipoR1)、腺苷酸激活蛋白激酶(AMPK)蛋白等相关指标的影响。方法:取50只健康大鼠,分为健康组,模型组,低、中、高剂量组,除健康组外均建立糖尿病模型。建模后低、中、高剂量组分别灌胃芪地糖肾颗粒1.31、2.62、5.24 g/kg; 健康组、模型组给予等量0.9%氯化钠溶液灌胃,1次/d,均干预30 d。采用ELISA检测血清高密度脂蛋白胆固醇(HDL-C)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG),酶联免疫法测定空腹血糖(FBG),放免法测定空腹胰岛素(FINS),免疫印迹与PCR分别检测脂联素受体1(AdipoR1)、腺苷酸激活蛋白激酶(AMPK)蛋白、肾小管间质p38激活丝裂原活化蛋白激酶(p38MAPK)。结果:与健康组比较,模型组大鼠血清中HDL-C及AdipoR1、AMPK、p38MAPK蛋白/mRNA降低,LDL-C、TC、TG、FBC、FINS均升高(P<0.05); 与模型组比较,低剂量组大鼠血清中HDL-C及AdipoR1、AMPK、p38MAPK蛋白/mRNA升高,LDL-C、TC、TG及FBC、FINS均降低(P<0.05); 与低剂量组比较,中剂量组大鼠血清中HDL-C及AdipoR1、AMPK、p38MAPK蛋白/mRNA升高,LDL-C、TC、TG及FBC、FINS均降低(P<0.05); 与中剂量组比较,高剂量组大鼠血清中HDL-C及AdipoR1、AMPK、p38MAPK蛋白/mRNA升高,LDL-C、TC、TG及FBC、FINS均降低(P<0.05)。结论:芪地糖肾颗粒可改善2型糖尿病的脂质代谢及胰岛素抵抗,可能与提高AdipoR1、AMPK、p38MAPK表达相关。
Abstract:
Objective:To explore the effect of Qidi Tangshen granule on insulin resistance,lipid metabolism,AdipoR1,AMPK protein and other related indicators in type 2 diabetes mellitus rats.Methods:A total of 50 healthy rats were divided into healthy group,model group,low,medium and high dose groups.Except the healthy group,diabetes models were established.After modeling,the low,medium,and high dose groups were respectively gavaged with 1.31,2.62,and 5.24 g/kg of Qidi Tangshen granules.The healthy group and model group were given an equal amount of 0.9% sodium chloride by gavage once a day,and both groups were intervened for 30 days.ELISA was used to detect serum high-density lipoprotein cholesterol(HDL-C),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),triglycerides(TG),enzyme-linked immunosorbent assay was used to measure fasting blood glucose(FBG),radioimmunoassay was used to measure fasting insulin(FINS),immunoblotting and PCR were used to detect adiponectin receptor 1(AdipoR1),adenylate activated protein kinase(AMPK)protein,and renal tubulointerstitial p38 activated mitogen activated protein kinase(p38MAPK).Results:Compared with the healthy group,the serum levels of HDL-C,AdipoR1,AMPK,and p38MAPK protein/mRNA in the model group rats decreased,while LDL-C,TC,TG,FBC,and FINS increased(P<0.05).Compared with the model group,the serum levels of HDL-C,AdipoR1,AMPK,and p38MAPK protein/mRNA in the low-dose group of rats increased,while LDL-C,TC,TG,FBC,and FINS decreased(P<0.05).Compared with the low-dose group,the serum levels of HDL-C,AdipoR1,AMPK,and p38MAPK protein/mRNA in the middle dose group increased,while LDL-C,TC,TG,FBC,and FINS decreased(P<0.05).Compared with the medium dose group,the serum levels of HDL-C,AdipoR1,AMPK,and p38MAPK protein/mRNA in the high-dose group of rats increased,while LDL-C,TC,TG,FBC,and FINS decreased(P<0.05).Conclusion:Qidi Tangshen granule can improve lipid metabolism and insulin resistance in type 2 diabetes,and the mechanism may be related to the increase of AdipoR1,AMPK,p38MAPK expression.

参考文献/References:

[1] 徐瑞颜,成金燕,李乐愚,等.复方番石榴合剂治疗超重肥胖型2型糖尿病疗效及对患者肠黏膜屏障功能的影响[J].陕西中医,2022,43(8):1045-1048.
[2] 卢昉,刘晓霞,梁佩玲,等.清热益气降糖方对2型糖尿病患者胰岛α、β细胞功能的影响[J].陕西中医,2021,42(10):1400-1403.
[3] BODIS K,BOMBRICH M,SCHON M,et al.Effects of TM6SF2 rs58542926 polymorphism on hepatocellular lipids and insulin resistance in early type 2 diabetes[J].Nutr Metab Cardiovasc Dis,2023,33(9):1785-1796.
[4] ZHANG N,LIU C,ZHANG Y,et al.Liraglutide regulates lipid metabolism via FGF21-LKB1-AMPK-ACC1 pathway in white adipose tissues and macrophage of type 2 diabetic mice[J].Biochem Biophys Res Commun,2021,548(9):120-126.
[5] KIM J H,LEE J O,MOON J W,et al.Laminarin from salicornia herbacea stimulates glucose uptake through AMPK-p38 MAPK pathways in L6 muscle cells[J].Natural Product Communications,2020,15(3):1934578.
[6] 柳红芳,张向伟.张景岳,等.真阴精气理论在糖尿病肾病治疗中的应用探讨[J].北京中医药大学学报,2016,39(1):5-9.
[7] 韩玉岭,柳红芳,娄锡恩,等.病证结合法治疗气阴两虚型糖尿病肾病Ⅳ期蛋白尿[J].长春中医药大学学报,2014,30(5):903-905.
[8] WEI H,WANG L,AN Z,et al.Qiditangshen granules modulated the gut microbiome composition and improved bile acid proles in a mouse model of diabetic nephropathy[J].Biomed & Pharmacother,2021,133(1):111061.
[9] 朱建忠,赵灿,乔跃兵,等.辣木黄酮对糖尿病脑病大鼠认知功能及神经病理指标的影响[J].中华危重病急救医学,2020,32(12):1491-1495.
[10] 赵雪杉,安至超,柳红芳,等.芪地糖肾颗粒对糖尿病肾病大鼠模型炎症因子IL-6、IL-18、TNF-α、CRP的影响[J].中国中西医结合肾病杂志,2020,21(1):8-11,95.
[11] 李秀钧.胰岛素抵抗综合征:胰岛素敏感性评估及应用[M].北京:人民卫生出版社,2001:44.
[12] HANLEY A,WILLIAMS K,GONZALEZ C,et al.Prediction of type 2 diabetes using simple measures of insulin resistance combined results from the san antonio heart study,the mexico city diabetes study,and the insulin resistance atherosclerosis study[J].Diabetes,2003,52(2):463.
[13] MEIJEL L A,VEGT F D,ABBINK E J,et al.High prevalence of impaired awareness of hypoglycemia and severe hypoglycemia among people with insulin-treated type 2 diabetes:The dutch diabetes pearl cohort[J].BMJ Open Diabetes Res Care,2020,8(1):935.
[14] 李亚丽.二甲双胍联合沙格列汀对2型糖尿病患者血糖控制效果及体脂分布的影响[J].河北医药,2022,44(19):2991-2993.
[15] 官杰,冯兴中.基于“虚气流滞”学说论糖尿病微血管病变的防治[J].中国中医基础医学杂志,2020,26(8):1065-1067.
[16] CHREITAH A,HIJAZIA K,DOYA L,et al.Severe dyslipidemia associated with diabetic ketoacidosis in newly diagnosed female of type 1 diabetes mellitus[J].Oxf Med Case Reports,2021,26(10):36.
[17] 郑锦标,马萍萍,马瑞倩,等.糖尿病伴发视网膜微血管异常临床特征及危险因素调查研究[J].陕西医学杂志,2019,48(11):1571-1573.
[18] 张志梅,费雯婕,齐翠娟,等.二甲双胍对2型糖尿病患者糖脂代谢、Socs3及炎性因子的影响[J].河北医药,2022,44(22):3384-3388.
[19] 高雪,柳红芳,安至超,等.芪地糖肾颗粒通过调节ERK信号通路减轻2型糖尿病肾病大鼠足细胞损伤的作用研究[J].中国中西医结合肾病杂志,2019,20(7):568-572,659.
[20] ELQADI M,EWEIDAT K,SABHA M A,et al.Methylenetetrahydrofolate reductase C677T gene polymorphism and the association with dyslipidemia in type 2 diabetic palestinian patients[J].J Clin Lab Anal,2021,35(10):23994.
[21] PETERSEN M,CSHULMAN G I.Mechanisms of insulin action and insulin resistance[J].Physiol Rev,2018,98(4):2133-2223.
[22] 何其英,柳红芳.芪地糖肾颗粒对糖尿病肾病大鼠肾组织TGF-β1-Smad2/3信号通路的影响[J].长春中医药大学学报,2019,35(6):1125-1128.
[23] 王琦,曲晓璐.肾芪1号方对早期糖尿病肾病胰岛素抵抗及尿微量白蛋白的影响[J].上海中医药杂志,2018,52(7):52-54.
[24] 都燕,任娟,罗苗苗,等.肌醇和维生素B、D对妊娠糖尿病小鼠炎症及脂代谢的影响及机制探讨[J].陕西医学杂志,2022,51(2):159-163.
[25] 丛金凤,范颖,李新,等.基于AMPK/SREBP1信号通路探究黄芪葛根汤有效组分对糖尿病大鼠脂代谢及炎症反应的作用机制[J].中华中医药学刊,2021,39(1):151-154.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(8160050038); 河北省医学科学研究项目(20200402)
更新日期/Last Update: 2024-02-07