[1]文 静,王理槐,侯 超.基于JAK2/STAT3通路研究周氏固金消瘤汤含药血清对人肺腺癌A549细胞侵袭迁移能力的影响[J].陕西中医,2024,(4):451-456.[doi:DOI:10.3969/j.issn.1000-7369.2024.04.004]
　WEN Jing,WANG Lihuai,HOU Chao.Effect of drug-containing serum of Zhoushi Gujin Xiaoliu decoction on migration and invasion of human lung adenocarcinoma A549 cells based on JAK2/STAT3 pathway[J].,2024,(4):451-456.[doi:DOI:10.3969/j.issn.1000-7369.2024.04.004]
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基于JAK2/STAT3通路研究周氏固金消瘤汤含药血清对人肺腺癌A549细胞侵袭迁移能力的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年4期
页码:
451-456
栏目:
基础研究
出版日期:
2024-04-05

文章信息/Info

Title:
Effect of drug-containing serum of Zhoushi Gujin Xiaoliu decoction on migration and invasion of human lung adenocarcinoma A549 cells based on JAK2/STAT3 pathway
作者:
文 静1; 王理槐1; 侯 超2
(1.湖南中医药大学第一附属医院,湖南 长沙 410007; 2.深圳市中医院,广东 深圳 518033)
Author(s):
WEN Jing; WANG Lihuai; HOU Chao
(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)
关键词:
肺腺癌A549细胞;  周氏固金消瘤汤;  含药血清;  侵袭;  迁移;  JAK2/STAT3
Keywords:
Adenocarcinoma of lung A549 cells;  Zhoushi Gujin Xiaoliu decoction;  Drug-containing serum;  Invasion;  Migration;  JAK2/STAT3
分类号:
R 734.2
DOI:
DOI:10.3969/j.issn.1000-7369.2024.04.004
文献标志码:
A
摘要:
目的:探讨周氏固金消瘤汤(ZSGJXLT)含药血清对人肺腺癌A549细胞侵袭迁移能力的影响及其机制。方法:应用健康SD大鼠制备ZSGJXLT含药血清与空白血清,将0%(空白血清)及5%、10%、20%、30%、40% ZSGJXLT含药血清作用于A549细胞,CCK-8法测算细胞活性抑制率和半数抑制浓度(IC50)。用0% ZSGJXLT(空白对照组)、5% ZSGJXLT(ZSGJXLT低剂量组)、10% ZSGJXLT(ZSGJXLT中剂量组)及20% ZSGJXLT(ZSGJXLT高剂量组)含药血清培养A549细胞,细胞划痕实验及高内涵细胞成像技术观察细胞运动情况; Transwell侵袭及迁移实验观察细胞侵袭及迁移情况; Western blot法检测A549细胞JAK2/STAT3通路相关蛋白表达情况。结果:与空白对照组相比,不同浓度ZSGJXLT含药血清组A549细胞活性受到不同程度的抑制,与时间及浓度呈正相关性,24 h的IC50为25.42%。与空白对照组相比,ZSGJXLT中、高剂量组A549细胞划痕愈合率、细胞移动速度均显著下降(均P<0.05),不同浓度ZSGJXLT含药血清组A549细胞侵袭及迁移数量明显减少(均P<0.05)。与空白对照组比较,ZSGJXLT中、高剂量组A549细胞的p-JAK2、p-STAT3、VEGFA蛋白表达水平显著降低(均P<0.05); 不同浓度ZSGJXLT含药血清组MMP-2、MMP-9、N-cadherin蛋白表达有下调趋势,而E-cadherin蛋白表达有上调趋势,呈剂量依赖性,其中ZSGJXLT高剂量组与空白对照组比较差异有统计学意义(均P<0.05)。结论:周氏固金消瘤汤含药血清能抑制A549细胞侵袭迁移能力,其机制可能与调控JAK2/STAT3通路有关。
Abstract:
Objective:To investigate the effect of Zhoushi Gujin Xiaoliu decoction(ZSGJXLT)medicated serum on the invasion and migration ability of human lung adenocarcinoma A549 cells and its mechanism.Methods:Healthy SD rats were applied to prepared ZSGJXLT containing serum.The serum containing 0%,5%,10%,20%,30% and 40% ZSGJXLT was used to culture A549 cells.The cell activity inhibition rate and IC50was evaluated by CCK-8 method.The drug-containing serum of 0% ZSGJXLT(blank control group),5% ZSGJXLT(low-dose group),10% ZSGJXLT(middle-dose group)and 20%ZSGJXLT(high-dose group)was used to culture A549 cells.The motility ability of cells was observed by wound healing assay and high-content imaging system analysis.The invasion and migration ability of cells was observed by Transwell invasion and migration assays.The expression of JAK2/STAT3 pathway related proteins were detected via Western blot.Results:Compared with the blank control group,the cell activity of A549 cells were inhibited by ZSGJXLT containing serum obviously in a concentration and time-dependent manner,and IC50 of 24 h was 25.42%.The wounding healing rate and movement speed of A549 cells decreased obviously cultured with middle-dose and high-dose ZSGJXLT containing serum(all P<0.05).The invasion and migration ability rate of A549 cells decreased obviously cultured with ZSGJXLT containing serum(all P<0.05).The expression of p-JAK2,p-STAT3,and VEGFA proteins decreased obviously cultured with middle-dose and high-dose ZSGJXLT containing serum(all P<0.05).The expression of MMP-2,MMP-9,and N-cadherin proteins decreased,but the expression of E-cadherin protein of A549 cells increased cultured with ZSGJXLT containing serum in a concentration-dependent manner,in which high-dose ZSGJXLT containing serum group had significant difference(all P<0.05).Conclusion:ZSGJXLT containing serum could inhibit the invasion and migration ability of A549 cells,which might be through inhibiting the activity of JAK2/STAT3 signal pathway.

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备注/Memo

备注/Memo:
基金项目:江苏省自然科学基金资助青年项目(BK20180277); 湖南省中医肿瘤临床研究中心项目(2021SK4023); 湖南省教育厅科学研究项目(21C0247); 广东省深圳市医疗卫生三名工程项目(SZZYSM202211003)
更新日期/Last Update: 2024-04-10