[1]赵丽蓉,黎 晨,黄正元,等.石榴补血糖浆对东莨菪碱诱导PC12细胞凋亡的保护作用及机制研究[J].陕西中医,2024,(5):588-592.[doi:DOI:10.3969/j.issn.1000-7369.2024.05.003]
　ZHAO Lirong,LI Chen,HUANG Zhengyuan,et al.Protective effect of Pomegranate Blood-enriching syrup on apoptosis of PC12 cells induced by scopolamine and its mechanism[J].,2024,(5):588-592.[doi:DOI:10.3969/j.issn.1000-7369.2024.05.003]
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石榴补血糖浆对东莨菪碱诱导PC12细胞凋亡的保护作用及机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年5期
页码:
588-592
栏目:
基础研究
出版日期:
2024-05-05

文章信息/Info

Title:
Protective effect of Pomegranate Blood-enriching syrup on apoptosis of PC12 cells induced by scopolamine and its mechanism
作者:
赵丽蓉1; 黎 晨1; 黄正元1; 尹海龙2; 成传芳2; 周本宏1
(1.武汉大学人民医院药学部,湖北 武汉 430060; 2.新疆维吾尔药业有限责任公司,新疆 乌鲁木齐 830026)
Author(s):
ZHAO Lirong; LI Chen; HUANG Zhengyuan; YIN Hailong; CHENG Chuanfang; ZHOU Benhong
(Department of Pharmacy,Renmin Hospital of Wuhan University,Wuhan 430060,China)
关键词:
石榴补血糖浆;  神经保护;  Bax/Bcl-2通路;  认知障碍;  细胞凋亡;  PC12细胞
Keywords:
Pomegranate Blood-enriching syrup;  Neuroprotection;  Bax/Bcl-2 signaling pathway;  Cognitive impairment;  Apoptosis;  PC12 cells
分类号:
R 749.1
DOI:
DOI:10.3969/j.issn.1000-7369.2024.05.003
文献标志码:
A
摘要:
目的:探讨石榴补血糖浆对东莨菪碱诱导大鼠肾上腺髓质嗜铬细胞瘤(PC12)细胞凋亡保护作用,探究石榴补血糖浆体外改善认知功能障碍的保护作用机制。方法:将不同浓度的石榴补血糖浆冻干粉细胞培养液作用于PC12细胞,采用CCK-8法检测细胞活性,根据细胞活性结果将后续实验分为空白对照组(NC)、模型组(SC)、石榴补血糖浆低、中、高剂量组(L、M、H:25、50、100 μg/ml)、多奈哌齐对照组(D,10 μmol/L); 利用ELISA试剂盒检测细胞乙酰胆碱(Ach)、乙酰胆碱酯酶(AchE)、超氧化物歧化酶(SOD)和脂质过氧化产物丙二醛(MDA)的水平,流式凋亡术检测活性氧自由基(ROS)及细胞凋亡情况; Western blot法检测凋亡相关蛋白Bcl-2、Bax、Caspase-3、Cleaved-Caspase-3等蛋白的表达情况。结果:石榴补血糖浆可显著增加PC12细胞的存活率,增加Ach、SOD的水平,降低ROS、MDA及AchE的水平,降低Bax/Bcl-2的比值和Cleaved-Caspase-3/Caspase-3蛋白的表达。结论:石榴补血糖浆可通过Bax/Bcl-2信号通路抑制细胞凋亡,减轻氧化应激,影响细胞内神经递质的水平发挥神经保护作用。
Abstract:
Objective:To investigate the protective effect of Pomegranate Blood-enriching syrup on apoptosis of PC12 cells induced by scopolamine and the protective mechanism of Pomegranate Blood-enriching syrup on Alzheimer's disease(AD)in vitro.Methods:PC12 cells were cultured with different concentrations of Pomegranate Blood-enriching syrup freeze dried powders,and the cell activity was detected by CCK-8.According to the results of cell activity,the subsequent experiments were divided into blank control group,model group,low,middle and high dose groups(25,50,100 μg/ml)and Donepezil control group(10 μg/ml).The levels of acetylcholine(Ach),acetylcholinesterase(AchE),superoxide dismutase(SOD)and malondialdehyde(MDA)were detected by ELISA kit,reactive oxygen species(ROS)and apoptosis were detected by flow cytometry,and the expressions of apoptosis-related proteins such as Bcl-2,Bax,Caspase-3 and Cleaved-Caspase-3 were detected by Western blotting.Results:Pomegranate Blood-enriching syrup could significantly increase the survival rate of PC12 cells,increased the levels of Ach and SOD,decrease the levels of ROS,MDA and AchE,decreased the expression of Bax and Cleaved-Caspase-3 protein,and increased the expression of Bcl-2 protein.Conclusion:Pomegranate Blood-enriching syrup can inhibit apoptosis and reduce oxidative stress through Bax/Bcl-2 signal pathway,and can also play a neuroprotective effect by affecting the level of neurotransmitters.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(31770381)
更新日期/Last Update: 2024-05-09