[1]张海洋,陈思鼎,施妙璇,等.基于AMPK/mTOR信号通路探讨人参-黄芪对人胃癌细胞自噬的影响[J].陕西中医,2024,(6):723-727.[doi:DOI:10.3969/j.issn.1000-7369.2024.06.001]
　ZHANG Haiyang,CHEN Siding,SHI Miaoxuan,et al.Exploring the effect of ginseng and astragalus on autophagy of human gastric cancer cells based on AMPK/mTOR signaling pathway[J].,2024,(6):723-727.[doi:DOI:10.3969/j.issn.1000-7369.2024.06.001]
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基于AMPK/mTOR信号通路探讨人参-黄芪对人胃癌细胞自噬的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年6期
页码:
723-727
栏目:
基础研究
出版日期:
2024-06-05

文章信息/Info

Title:
Exploring the effect of ginseng and astragalus on autophagy of human gastric cancer cells based on AMPK/mTOR signaling pathway
作者:
张海洋1; 陈思鼎1; 施妙璇1; 郑 薇1; 韩美奕1; 丁治国1; 季双双2; 田 明1
(1.北京中医药大学东直门医院,北京 100700; 2.密歇根大学安娜堡分校胃肠病学与肝病学部,美国 密歇根州 MI 48105)
Author(s):
ZHANG Haiyang; CHEN Siding; SHI Miaoxuan; ZHENG Wei; HAN Meiyi; DING Zhiguo; JI Shuangshuang; TIAN Ming
(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)
关键词:
胃癌;  人参;  黄芪;  腺苷酸激活蛋白激酶;  雷帕霉素靶蛋白;  自噬
Keywords:
Gastric cancer;  Ginseng;  Astragalus;  Adenosine monophosphate-activated protein kinase;  Mammalian target of rapamycin;  Autophagy
分类号:
R 735.2
DOI:
DOI:10.3969/j.issn.1000-7369.2024.06.001
文献标志码:
A
摘要:
目的:探究益气扶正药人参-黄芪对人胃癌细胞自噬的影响,并探讨腺苷酸活化蛋白激酶(AMPK)/哺乳动物雷帕霉素靶蛋白(mTOR)信号通路在其中的作用机制。方法:将人胃癌细胞株(SGC-7901)随机分为对照组(人参-黄芪 0 mg/ml)和人参-黄芪组(1、2、4、8、16、32、64 mg/ml),经药物处理后,CCK-8法观察人胃癌细胞增殖情况; 筛选最佳浓度并将其作为后续实验中的实验组; MDC免疫荧光染色观察细胞自噬情况; Western blot法检测人胃癌细胞自噬相关蛋白(Beclin-1、LC3B)及AMPK/mTOR信号通路相关蛋白(AMPK、p-AMPK、mTOR及p-mTOR)的表达情况。结果:CCK-8法结果显示,人参-黄芪组人胃癌细胞增殖作用降低(P<0.01),且呈浓度依赖性; MDC染色荧光检测显示实验组的荧光强度显著增强(P<0.01),表明人参-黄芪可以诱导人胃癌细胞产生自噬; Western blot结果显示,实验组的Beclin-1、LC3B、p-AMPK/AMPK蛋白表达水平上升(P<0.01),p-mTOR/mTOR蛋白表达水平下降(P<0.01)。结论:人参-黄芪配伍能够抑制人胃癌细胞的增殖、促进人胃癌细胞自噬,其机制可能与激活AMPK/mTOR信号通路相关。
Abstract:
Objective:To investigate the effect of ginseng and astragalus on autophagy of human gastric cancer cells,and to explore the mechanism of adenosine monophosphate-activated protein kinase(AMPK)/mammalian target of rapamycin(mTOR)signaling pathway in this process.Methods:The human gastric cancer cell lines(SGC-7901)were randomly divided into control group(ginseng and astragalus 0 mg/ml)and ginseng and astragalus groups(1,2,4,8,16,32,64 mg/ml).After medicine treatment,the proliferation of human gastric cancer cells was observed by CCK-8 method.The optimal concentration was screened and used as the experimental group in the subsequent experiments.The autophagy of the cells was observed by MDC immunofluorescence staining.The expressions of autophagy-related proteins(Beclin-1,LC3B)and AMPK/mTOR signaling pathway-related proteins(AMPK,p-AMPK,mTOR and p-mTOR)in human gastric cancer cells were detected by Western blot.Results:The results of CCK-8 showed that the proliferation of human gastric cancer cells in ginseng and astragalus group was decreased(P<0.01),and the proliferation was concentration-dependent.MDC fluorescence detection showed that the fluorescence intensity of the experimental group was significantly enhanced(P<0.01),indicating that ginseng and astragalus could induce autophagy in human gastric cancer cells.Western blot results showed that the protein expression levels of Beclin-1,LC3B and p-AMPK/AMPK in the experimental group increased(P<0.01),while the protein expression levels of p-mTOR/mTOR decreased(P<0.01).Conclusion:The combination of ginseng and Astragalus can inhibit the proliferation and promote the autophagy of human gastric cancer cells.The activation of the AMPK/mTOR signaling pathway may underlie this mechanism.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81673972); 北京中医药大学青年教师科研项目(2018-JYBZZ-JS081)
更新日期/Last Update: 2024-06-11