[1]刘 博,张成奇,董 青,等.化瘀降浊方治疗痰湿型多囊卵巢综合征机制研究[J].陕西中医,2024,(7):888-892.[doi:DOI:10.3969/j.issn.1000-7369.2024.07.005]
　LIU Bo,ZHANG Chengqi,DONG Qing,et al.Mechanism study on treatment of Tanshi type polycystic ovarian syndrome with Huayu Jiangzhuo formula[J].,2024,(7):888-892.[doi:DOI:10.3969/j.issn.1000-7369.2024.07.005]
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化瘀降浊方治疗痰湿型多囊卵巢综合征机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年7期
页码:
888-892
栏目:
基础研究
出版日期:
2024-07-05

文章信息/Info

Title:
Mechanism study on treatment of Tanshi type polycystic ovarian syndrome with Huayu Jiangzhuo formula
作者:
刘 博; 张成奇; 董 青; 魏 萱; 王 娟; 梁贵廷
(石家庄市中医院内分泌科,河北 石家庄 050051)
Author(s):
LIU Bo; ZHANG Chengqi; DONG Qing; WEI Xuan; WANG Juan; LIANG Guiting
(Department of Endocrinology,Shijiazhuang Traditional Chinese Medicine Hospital,Shijiazhuang 050051,China)
关键词:
多囊卵巢综合征;  调补脾胃法;  痰湿型;  炎症;  Toll样受体4;  核转录因子κB
Keywords:
Polycystic ovary syndrome;  Tiaobu Piwei method;  Tanshi type;  Inflammation;  TLR4;  NF-κB
分类号:
R 711.75
DOI:
DOI:10.3969/j.issn.1000-7369.2024.07.005
文献标志码:
A
摘要:
目的:探讨化瘀降浊方对痰湿型多囊卵巢综合征(PCOS)大鼠的治疗作用及机制。方法:采用高脂饮食联合来曲唑灌胃法构建痰湿型PCOS大鼠模型,将50只大鼠随机分为对照组、模型组、二甲双胍组、低剂量化瘀降浊方组、高剂量化瘀降浊方组。采用ELISA法检测大鼠血清中激素水平; 采用HE染色检测大鼠卵巢组织病理变化; 利用Western blot法检测TLR4/NF-κB p56信号通路蛋白变化。结果:干预后,与模型组比较,二甲双胍组、低剂量化瘀降浊方组、高剂量化瘀降浊方组大鼠的体重及Lee's指数均较低(P<0.05)。与对照组相比,模型组大鼠的睾酮(T)、促黄体生成素(LH)、LH/FSH水平显著升高,雌二醇(E2)、孕酮(P)、促卵泡激素(FSH)水平较低(P<0.05)。与模型组比较,干预后二甲双胍组、低剂量化瘀降浊方组、高剂量化瘀降浊方组大鼠的T、LH、LH/FSH水平降低(P<0.05),而E2、P、FSH水平升高(P<0.05)。与对照组比较,模型组大鼠卵巢组织中TLR4、MyD88、NF-κB p65蛋白相对表达量增高(P<0.05)。与模型组比较,二甲双胍组、低剂量化瘀降浊方组、高剂量化瘀降浊方组大鼠卵巢组织中TLR4、MyD88、NF-κB p65蛋白相对表达量降低(P<0.05)。结论:化瘀降浊方通过抑制TLR4/NF-κB p56信号通路活性调节PCOS大鼠性激素分泌水平,改善卵巢组织病理损伤。
Abstract:
Objective:Exploring therapeutic effect and mechanism of Huayu Jiangzhuo formula on rats with phlegm dampness type polycystic ovary syndrome(PCOS).Methods:Rat model of phlegm dampness type PCOS constructed using high-fat diet and letrozole gavage.Fifty rats were randomly divided into control group,model group,metformin group,low-dose Huayu Jiangzhuo formula group,high-dose Huayu Jiangzhuo formula group.Using ELISA method to detect hormone levels in rat serum.HE staining was used to detect pathological changes in rat ovarian tissue.Western blot to detect changes in TLR4/NF-κB p56 signaling pathway protein expression levels.Results:After intervention,compared with the model group,body weight and Lee's index of rats in the metformin group,low-dose Huayu Jiangzhuo formula group,high-dose Huayu Jiangzhuo formula group were significantly lower(P<0.05).Compared with the control group,the levels of testosterone(T),luteinizing hormone(LH),and LH/FSH in the model group rats were significantly higher,while the levels of estradiol(E2),progesterone(P),and follicle stimulating hormone(FSH)were significantly lower(P<0.05).Compared with the model group,after intervention,the T,LH,LH/FSH levels of rats in the metformin group,low-dose Huayu Jiangzhuo formula group and high-dose Huayu Jiangzhuo formula group decreased(P<0.05),while the E2,P,and FSH levels increased(P<0.05).Compared with the control group,the relative expression levels of TLR4,MyD88,and NF-κB p65 proteins in the ovarian tissue of the model group rats increased(P<0.05).Compared with the model group,the relative expression levels of TLR4,MyD88,and NF-κB p65 proteins in the ovarian tissue of rats in the metformin group,low-dose Huayu Jiangzhuo formula group and high-dose Huayu Jiangzhuo formula group were reduced(P<0.05).Conclusion:Huayu Jiangzhuo formula can regulate secretion level of sex hormones in PCOS rats by inhibiting activity of TLR4/NF-κB p56 signaling pathway and improve ovarian tissue pathological damage.

参考文献/References:

[1] SADEGHI H M,ADELI I,CALINA D,et al.Polycystic ovary syndrome:A comprehensive review of pathogenesis management and drug repurposing[J].Int Journal Mol Sci,2022,23(2):583.
[2] TEEDE H J,TAY C T,LAVEN J E,et al.Recommendations from the 2023 international evidence-based guideline for the assessment and management of polycystic ovary syndrome[J].Eur Journal Endocrinol,2023,189(2):43-64.
[3] RASHID R,MIR S A,KAREEM O,et al.Polycystic ovarian syndrome-current pharmacotherapy and clinical implications[J].Taiwan Journal Obstet Gynecol,2022,61(1):40-50.
[4] YANG Y,YANG L,QI C,et al.Cryptotanshinone alleviates polycystic ovary syndrome in rats by regulating the HMGB1/TLR4/NF-κB signaling pathway[J].Mol Med Rep,2020,22(5):3851-3861.
[5] JIANG B,XUE M,XU D,et al.Retracted article:Upregulation of microRNA-204 improves insulin resistance of polycystic ovarian syndrome via inhibition of HMGB1 and the inactivation of the TLR4/NF-κB pathway[J].Cell Cycle,2020,19(6):697-710.
[6] HU M,ZHANG Y,LI X,et al.TLR4-associated IRF-7 and NF-κB signaling act as a molecular link between androgen and metformin activities and cytokine synthesis in the PCOS endometrium[J].Journal Clin Endocrinol Meta,2021,106(4):e1022-e1040.
[7] 滕婧,杨柳.祛湿化痰方联合针刺治疗脾虚痰湿型多囊卵巢综合征疗效研究[J].陕西中医,2023,44(12):1813-1818.
[8] 李延晖,袁戈.自拟调经汤治疗肾虚痰湿型多囊卵巢综合征疗效及对中医证候、内分泌和糖脂代谢的影响[J].现代中西医结合杂志,2019,28(17):1861-1865.
[9] 张红阳,侯丽辉,孙淼.多囊卵巢综合征患者西医亚型与中医证型的相关研究[J].现代中西医结合杂志,2019,28(17):1829-1833.
[10] FORNES R,SIMIN J,NGUYEN M H,et al.Pregnancyperinatal and childhood outcomes in women with and without polycystic ovary syndrome and metformin during pregnancy:A nationwide population-based study[J].Reprod Biol Endocrinol,2022,20(1):1-12.
[11] KAZEMI M,KIM J Y,WAN C,et al.Comparison of dietary and physical activity behaviors in women with and without polycystic ovary syndrome:A systematic review and meta-analysis of 39471 women[J].Hum Reprod Update,2022,28(6):910-955.
[12] HU L,MA L,XIA X,et al.Efficacy of bariatric surgery in the treatment of women with obesity and polycystic ovary syndrome[J].Journal Clin Endocrinol Metab,2022,107(8):e3217-e3229.
[13] LYLE S M,AHMED S,ELLIOTT J E,et al.Transcriptome-wide association analyses identify an association between ARL14EP and polycystic ovary syndrome[J].Journal Hum Genet,2023,68(5):347-353.
[14] 罗成龙,苏亮,张思政,等.焦拥政基于“酒性辛燥”从气血精论治酒伤阳痿经验[J].中国医药导报,2023,20(22):110-113.
[15] 刘丹,姚海洋,滕秀香.国医大师柴松岩闭经验案举隅[J].中国生育健康杂志,2020,31(1):60-63.
[16] 崔宇胜,纪云西.中西医治疗非酒精性脂肪性肝炎的研究进展[J].中西医结合肝病杂志,2021,31(1):702-706.
[17] 麦静,陈建杰.陈建杰教授分期论治非酒精性脂肪性肝病的经验报道[J].中国中西医结合杂志,2019,39(10):1263-1265.
[18] 郑允玲,朱玲.朱玲基于“病痰饮者,当以温药和之”治疗多囊卵巢综合征的经验[J].广州中医药大学学报,2024,41(1):224-229.
[19] 岳美宏,姜大成,雷钧涛.化痰祛湿方对肥胖型多囊卵巢综合征大鼠的改善作用及对TGF-β/CTGF通路的影响[J].中国中医基础医学杂志,2023,29(8):1279-1283.
[20] 廖朝青,钟海英,夏淑芳,等.祛湿化痰助孕方联合枸橼酸氯米芬对痰湿困阻型多囊卵巢综合征患者的临床疗效[J].中成药,2022,44(12):3858-3861.
[21] LIN C,WANG H,ZHANG M,et al.TLR4 biased small molecule modulators[J].Pharmacol Ther,2021,228:107918.
[22] SHEN H R,XU X,LI X L.Berberine exerts a protective effect on rats with polycystic ovary syndrome by inhibiting the inflammatory response and cell apoptosis[J].Reprod Biol Endocrinol,2021,19(1):1-11.
[23] 杨芳,高素文,刘旋,等.丹参酮ⅡA通过调控p38MAPK/NF-κB信号通路减轻糖尿病雄性大鼠生殖损伤实验研究[J].陕西医学杂志,2024,53(5):610-615.
[24] 李建英,王娟红,鱼军,等.核转录因子NF-κB、RET/PTC在桥本甲状腺炎合并甲状腺乳头状癌中的表达及其意义[J].陕西医学杂志,2017,46(10):1326-1328,1334.
[25] ALA M.Metformin for cardiovascular protectioninflammatory bowel disease osteoporosis periodontitis polycystic ovarian syndrome neurodegeneration cancer inflammation and senescence:What is next?[J].ACS Pharmacol Transl Sci,2021,4(6):1747-1770.
[26] REN Y,YE Y,XUAN F,et al.The effect of sitagliptin combined with rosiglitazone on autophagy and inflammation in polycystic ovary syndrome by regulating PI3K/AKT/mTOR and TLR4/NF-κB pathway[J].Reprod Biol,2023,23(2):100763.
[27] CHANG Z P,DENG G F,SHAO Y Y,et al.Shaoyao Gancao decoction ameliorates the inflammation state in polycystic ovary syndrome rats via remodeling gut microbiota and suppressing the TLR4/NF-κB pathway[J].Front Pharmacol,2021,12:670054.
[28] 吴文婷,李敏,蒲文娟.白藜芦醇对慢性阻塞性肺疾病大鼠p38丝裂原活化蛋白激酶/核因子-κB信号通路的影响[J].陕西医学杂志,2023,52(11):1489-1492,1534.
[29] 董鹂芸,丛萍,白俊,等.苍砂白芥汤结合GnRH-a治疗子宫肌瘤疗效及对其卵巢功能、血清bFGF、NF-κB水平影响[J].现代生物医学进展,2022,22(23):4551-4554,4520.
[30] 兰永利,周知然,韦芳,等.针灸预处理调控TLR4/MyD88/NF-κB信号通路抗大鼠胃黏膜损伤机制研究[J].陕西中医,2023,44(10):1349-1353,1359.

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备注/Memo

备注/Memo:
基金项目:河北省中医药管理局科研计划项目(2019444)
更新日期/Last Update: 2024-07-09