[1]周丹丹,马 晨,何伟坤,等.针刺足三里对小鼠术后肠麻痹的作用机制[J].陕西中医,2024,(7):893-896.[doi:DOI:10.3969/j.issn.1000-7369.2024.07.006]
　ZHOU Dandan,MA Chen,HE Weikun,et al.Mechanism of acupuncture at Zusanli point in treating postoperative ileus[J].,2024,(7):893-896.[doi:DOI:10.3969/j.issn.1000-7369.2024.07.006]
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针刺足三里对小鼠术后肠麻痹的作用机制
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年7期
页码:
893-896
栏目:
基础研究
出版日期:
2024-07-05

文章信息/Info

Title:
Mechanism of acupuncture at Zusanli point in treating postoperative ileus
作者:
周丹丹; 马 晨; 何伟坤; 范 哲
(西北妇女儿童医院麻醉科,陕西 西安 710061)
Author(s):
ZHOU Dandan; MA Chen; HE Weikun; FAN Zhe
(Department of Anesthesiology,Northwest Women's and Children's Hospital,Xi'an 710061,China)
关键词:
术后肠麻痹;  针刺;  电针;  足三里;  Toll样受体4;  围手术期
Keywords:
Postoperative ileus;  Acupuncture;  Electroacupuncture;  Zusanli;  Toll like receptor 4;  Perioperative period
分类号:
R 574
DOI:
DOI:10.3969/j.issn.1000-7369.2024.07.006
文献标志码:
A
摘要:
目的:本研究旨在探索针刺足三里穴位治疗术后肠麻痹的作用及其信号传导机制。方法:使用野生型小鼠和Toll样受体4基因敲除小鼠各10只构建术后肠麻痹模型。比较Toll样受体4基因敲除小鼠、野生型小鼠术后胃肠运动功能、肠黏膜损伤情况。使用20只野生型小鼠构建术后肠麻痹模型,随机分为电针足三里穴位组和足三里穴位对照组,每组10只,电针足三里穴位组在造模后6、24、48、72 h连续足三里穴位电针刺激4次,足三里穴位对照组连接电针仪但不给予电刺激,检测两组实验动物胃肠功能运动情况、小肠组织炎症因子水平和Toll样受体相关通路关键分子TLR4、TRAF6及JNK表达。结果:与野生型小鼠相比,Toll样受体4基因敲除小鼠的胃肠运动功能改善,小肠组织炎症因子表达下调,肠黏膜损伤较轻。给予电针足三里穴位刺激可改善胃肠运动功能,减轻小肠组织炎症因子表达水平,并下调Toll样受体4相关因子TLR4、TRAF6和JNK表达水平。结论:电针足三里穴位可减轻术后肠麻痹,Toll样受体4信号通路在其中可能发挥关键调节作用。
Abstract:
Objective:Study aims to explore effects of acupuncture at Zusanli acupoint on postoperative ileus and its information transmission mechanism.Methods:Ten wild-type mice and ten Toll like receptor 4 gene knockout mice were used to construct postoperative ileus models.The postoperative gastrointestinal motility and intestinal mucosal damage were compared.Twenty wild-type mice were used to construct postoperative ileus model,which were randomly divided into electroacupuncture Zusanli acupoint group and control group,with ten mice in each group.The electroacupuncture Zusanli acupoint group were continuously stimulated with electroacupuncture at Zusanli acupoint 4 times at 6 h,24 h,48 h,and 72 h after modeling.The control group were connected to electroacupuncture instrument but not given electrical stimulation.The gastrointestinal motility function,levels of inflammatory factors in small intestine tissue,and key molecule Toll like receptor 4 related to Toll like receptor pathways in both groups were detected TRAF6 and JNK expression.Results:Compared with wild-type mice,Toll like receptor 4 gene knockout mice showed improved gastrointestinal motility,down-regulated expression of inflammatory factors in small intestine tissue,and milder intestinal mucosal damage.Stimulating Zusanli acupoint with electroacupuncture could improve gastrointestinal motility,reduce expression levels of inflammatory factors in small intestine tissue,and down-regulate the expression levels of Toll like receptor 4 related factorsTLR4,TRAF6 and JNK.Conclusion:Electroacupuncture at Zusanli acupoint could alleviate postoperative intestinal paralysis,and the Toll like receptor 4 signaling pathway might play key regulatory role in the process.

参考文献/References:

[1] WELLS C I,MILNE T G E,SEO S H B,et al.Post-operative ileus:Definitions mechanisms and controversies[J].ANZ Journal Surg,2021,9(22):1103-1107.
[2] ZHOU D,HU B,HE S,et al.Transcutaneous electrical acupoint stimulation accelerates the recovery of gastrointestinal function after cesarean section:A randomized controlled trial[J].Evid Based Complement Alternat Med,2018,13(6):7341920.
[3] FARRO G,GOMEZ P J,DI G M,et al.Smooth muscle and neural dysfunction contribute to different phases of murine postoperative ileus[J].Neurogastroenterol Motil,2016,28(6):934-947.
[4] LIN S S,ZHANG R Q,SHEN L,et al.Alterations in the gut barrier and involvement of Toll-like receptor 4 in murine postoperative ileus[J].Neurogastroenterol Motil,2018,30(6):e13286.
[5] 汪夏云,吴茜,汤爱洁,等.结直肠手术患者延迟性术后肠麻痹危险因素的Meta分析[J].中华现代护理杂志,2022,28(14):7-11.
[6] 黄越秀,陈光伟,张家墉,等.陈光伟教授治疗肿瘤根治术后肠麻痹经验[J].陕西中医,2020,41(11):1654-1656.
[7] 刘嘉芬,黄白沙,潘碧琦,等.大黄牡丹汤保留灌肠对小儿急性阑尾炎腹腔镜术后围手术期加速康复疗效观察[J].陕西中医,2020,41(7):923-925.
[8] GONG W,QI X.Association of frailty with delayed recovery of gastrointestinal function after elective colorectal cancer resection[J].Journal Invest Surg,2020,33(6):544-550.
[9] 杜嘉原,汪富涛.腹腔镜阑尾切除术治疗急性阑尾炎临床疗效及术后并发症研究[J].陕西医学杂志,2019,48(2):179-182.
[10] TENG C Y,MYERS S,KENKRE T S,et al.Targets for intervention preoperative predictors of postoperative ileus after colorectal surgery in an enhanced recovery protocol[J].Journal Gastrointest Surg,2021,25(8):2065-2075.
[11] IYER S,SAUNDERS W B,STEMKOWSKI S.Economic burden of postoperative ileus associated with colectomy in the United States[J].Care Pharm,2009,15(3):485-494.
[12] 李玉才,王群,张维亮,等.针刺改善术后胃肠功能紊乱研究进展[J].辽宁中医药大学学报,2023,25(5):107-112.
[13] 宫彩霞,张勤.中药对胃肠动力紊乱治疗作用的研究进展[J].中医药学报,2022,50(1):6-11.
[14] 代清泉,王宽宇.神阙穴用于治疗术后胃肠功能紊乱的研究进展[J].中国中医药科技,2023,30(5):1038-1040.
[15] 罗芳丽,雷枭,廖伯年,等.术后胃肠功能紊乱的中医治疗进展[J].中医药导报,2022,28(4):197-200.
[16] ZHANG T,XU Y,YAO Y,et al.Perioperative dexamethasone reduces excessive postoperative inflammatory response and ileus after surgery for inflammatory bowel disease[J].Inflamm Bowel Dis,2021,27(11):1756-1765.
[17] WEHNER S,STRAESSER S,VILZ T O,et al.Inhibition of p38 Mitogen-activated protein kinase pathway as prophylaxis of postoperative ileus in mice[J].Gastroenterology,2009,136(2):619-629.
[18] ZHANG R Q,LIN S I.Role of JNK signaling pathway in murine postoperative ileus[J].Journal of Tongji University,2019,1(7):34-39.
[19] ROCHA D M,CALDAS A P,OLIVEIRA L L,et al.Saturated fatty acids trigger TLR4-mediated inflammatory response[J].Atherosclerosis,2016,24(4):211-215.
[20] LATORRE E,LAYUNTA E,GRASA L,et al.Toll-like receptors 2 and 4 modulate intestinal IL-10 differently in the ileum and colon[J].United European Gastroenterology Journal,2018,65(41):446-453.
[21] MA Z,ZHANG Z,BAI F,et al.Electroacupuncture pretreatment alleviates cerebral ischemic injury through α7 nicotinic acetylcholine receptor-mediated phenotypic conversion of microglia[J].Front Cell Neurosci,2019,13(6):537-542.
[22] 黄谦峰,郭素真.从扶正治神角度探讨五行针灸的作用机制[J].中医研究,2023,36(8):8-11.
[23] 庄语,路玫,于冬冬,等.针灸通过调控凋亡因子半胱氨酸蛋白酶家族改善小鼠顺铂致肝损伤的作用机制[J].针刺研究,2022,47(7):6-12.
[24] 郭珊珊,傅心昊,张鑫,等.针灸治疗消化系统适宜病症的现代文献研究[J].中国中医基础医学杂志,2020,26(5):135-141.
[25] 兰永利,周知然,韦芳,等.针灸预处理调控TLR4/MyD88/NF-κB信号通路抗小鼠胃黏膜损伤机制研究[J].陕西中医,2023,44(10):1349-1353,1359.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基金资助青年项目(2021-JQ937)
更新日期/Last Update: 2024-07-09